Restriction map for
pFN21AE3993
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI AlwNI
Aor51HI ApoI AscI AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI HphI
KasI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 448 GGTACC G^GTACC
AccI 782 GTCGAC GT^[AC][GT]AC
AccII 60 CGCG CG^CG
AfaI 449 GTAC GT^AC
Alw44I 686 GTGCAC G^TGCAC
AlwI 214 GATCC GGATC(4/5)
AocI 666 CCTTAGG CC^T[ACGT]AGG
ApaI 278 GGGCCC GGGCC^C
ApaLI 686 GTGCAC G^TGCAC
AseI 328 ATTAAT AT^TAAT
AsnI 328 ATTAAT AT^TAAT
Asp700I 537 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 448 GGTACC G^GTACC
AvaII 510 GGACC G^G[AT]CC
BanI 448 GGTACC G^G[CT][GA]CC
BbsI 634 GAAGAC GAAGAC(2/6)
BbvCI 421 GCTGAGG CCTCAGC(-5/-2)
BclI 563 TGATCA T^GATCA
BcnI 276 CCGGG CC^[GC]GG
BglI 58 GCCGCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 634 GAAGAC GAAGAC(2/6)
BsaJI 59 CCGCGG C^C[ACGT][ACGT]GG
BseRI 403 CTCCTC GAGGAG(10/8)
BsiYI 360 CCTCCATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 360 CCTCCATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 386 GGGAC GGGAC(10/14)
BstUI 60 CGCG CG^CG
Bsu36I 666 CCTTAGG CC^T[ACGT]AGG
DrdI 388 GACTCATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 59 CCGCGG C^C[GA][CT]GG
Eco81I 666 CCTTAGG CC^T[ACGT]AGG
EcoT22I 268 ATGCAT ATGCA^T
FauI 281 CCCGC CCCGC(4/6)
FbaI 563 TGATCA T^GATCA
KpnI 448 GGTACC GGTAC^C
KspI 59 CCGCGG CCGC^GG
MslI 813 CACAAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 59 CCGCGG C[AC]G^C[GT]G
MvnI 60 CGCG CG^CG
NciI 276 CCGGG CC^[GC]GG
NsiI 268 ATGCAT ATGCA^T
PshBI 328 ATTAAT AT^TAAT
PstI 161 CTGCAG CTGCA^G
RsaI 449 GTAC GT^AC
SacI 31 GAGCTC GAGCT^C
SacII 59 CCGCGG CCGC^GG
SalI 782 GTCGAC G^TCGAC
SapI 250 GCTCTTC GCTCTTC(1/4)
Tsp45I 811 GTCAC ^GT[GC]AC
Tsp509I 703 AATT ^AATT
XbaI 198 TCTAGA T^CTAGA
XmnI 537 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AspHI 31 GAGCTC G[AT]GC[AT]^C
686 GTGCAC
BanII 31 GAGCTC G[GA]GC[CT]^C
278 GGGCCC
BfaI 100 CTAG C^TAG
199 CTAG
BpmI 34 CTCCAG CTGGAG(16/14)
409 CTGGAG
BsiHKAI 31 GAGCTC G[AT]GC[AT]^C
686 GTGCAC
BsrDI 285 CATTGC GCAATG(2/0)
341 GCAATG
DpnII 214 GATC ^GATC
564 GATC
DraIII 82 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
434 CACTCAGTG
EaeI 123 TGGCCG [CT]^GGCC[GA]
273 TGGCCG
EarI 251 CTCTTC CTCTTC(1/4)
40 GAAGAG
HapII 276 CCGG C^CGG
399 CCGG
HincII 427 GTTGAC GT[CT]^[GA]AC
782 GTCGAC
HindII 427 GTTGAC GT[CT]^[GA]AC
782 GTCGAC
HpaII 276 CCGG C^CGG
399 CCGG
Ksp632I 251 CTCTTC CTCTTC(1/4)
40 GAAGAG
MaeI 100 CTAG C^TAG
199 CTAG
MaeIII 3 GTTAC ^GT[ACGT]AC
811 GTCAC
MboI 214 GATC ^GATC
564 GATC
MspI 276 CCGG C^CGG
399 CCGG
NdeII 214 GATC ^GATC
564 GATC
Sau3AI 214 GATC ^GATC
564 GATC
SfaNI 96 GATGC GCATC(5/9)
130 GATGC
SfcI 161 CTGCAG C^T[GA][CT]AG
218 CTACAG
TfiI 51 GATTC G^A[AT]TC
304 GAATC
TspRI 86 CAGTG CA[GC]TG[ACGT][ACGT]^
438 CAGTG
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI BbvI BmyI Bsp1286I
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MboII MnlI MseI
MvaI MwoI NlaIII NlaIV PleI
Sau96I ScrFI TaqI Tru9I TseI
TthHB8I