Restriction map for
pFN21AE3993
(Linear) (Six-base) MAPSORT of: KIEE3993.seq Check: 5040 from: 1 to: 822
KIEE3993.orf
With 182 enzymes: SgfI *
March 22, 2012 17:05 ..
AccI GT'mk_AC
Cuts at: 0 783 822
Size: 783 39
Acc65I G'GTAC_C
Cuts at: 0 448 822
Size: 448 374
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 583 822
Size: 445 138 239
Fragments arranged by size:
445 239 138
ApaI G_GGCC'C
Cuts at: 0 282 822
Size: 282 540
ApaLI G'TGCA_C
Cuts at: 0 686 822
Size: 686 136
AseI AT'TA_AT
Cuts at: 0 329 822
Size: 329 493
BanI G'GyrC_C
Cuts at: 0 448 822
Size: 448 374
BanII G_rGCy'C
Cuts at: 0 35 282 822
Size: 35 247 540
Fragments arranged by size:
540 247 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 641 822
Size: 641 181
BbvCI CC'TCA_GC
Cuts at: 0 422 822
Size: 422 400
BclI T'GATC_A
Cuts at: 0 563 822
Size: 563 259
BfrBI ATG'CAT
Cuts at: 0 270 822
Size: 270 552
BglI GCCn_nnn'nGGC
Cuts at: 0 64 822
Size: 64 758
Bme1580I G_kGCm'C
Cuts at: 0 282 690 822
Size: 282 408 132
Fragments arranged by size:
408 282 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 430 822
Size: 19 411 392
Fragments arranged by size:
411 392 19
Bpu10I CC'TnA_GC
Cuts at: 0 29 422 822
Size: 29 393 400
Fragments arranged by size:
400 393 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 394 822
Size: 394 428
BseYI C'CCAG_C
Cuts at: 0 70 822
Size: 70 752
BsiHKAI G_wGCw'C
Cuts at: 0 35 690 822
Size: 35 655 132
Fragments arranged by size:
655 132 35
Bsp1286I G_dGCh'C
Cuts at: 0 35 282 690 822
Size: 35 247 408 132
Fragments arranged by size:
408 247 132 35
BsrDI GCAATG_nn'
Cuts at: 0 284 348 822
Size: 284 64 474
Fragments arranged by size:
474 284 64
Bsu36I CC'TnA_GG
Cuts at: 0 667 822
Size: 667 155
BtgI C'CryG_G
Cuts at: 0 59 822
Size: 59 763
DraIII CAC_nnn'GTG
Cuts at: 0 87 439 822
Size: 87 352 383
Fragments arranged by size:
383 352 87
DrdI GACnn_nn'nnGTC
Cuts at: 0 394 822
Size: 394 428
EaeI y'GGCC_r
Cuts at: 0 123 273 822
Size: 123 150 549
Fragments arranged by size:
549 150 123
EarI CTCTTCn'nnn_
Cuts at: 0 35 257 822
Size: 35 222 565
Fragments arranged by size:
565 222 35
EcoICRI GAG'CTC
Cuts at: 0 33 822
Size: 33 789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 430 445 583 822
Size: 19 411 15 138 239
Fragments arranged by size:
411 239 138 19 15
HincII GTy'rAC
Cuts at: 0 429 784 822
Size: 429 355 38
Fragments arranged by size:
429 355 38
KpnI G_GTAC'C
Cuts at: 0 452 822
Size: 452 370
MslI CAynn'nnrTG
Cuts at: 0 817 822
Size: 817 5
MspA1I CmG'CkG
Cuts at: 0 61 822
Size: 61 761
NsiI A_TGCA'T
Cuts at: 0 272 822
Size: 272 550
PfoI T'CCnGG_A
Cuts at: 0 35 407 506 822
Size: 35 372 99 316
Fragments arranged by size:
372 316 99 35
PspOMI G'GGCC_C
Cuts at: 0 278 822
Size: 278 544
PstI C_TGCA'G
Cuts at: 0 165 822
Size: 165 657
SacI G_AGCT'C
Cuts at: 0 35 822
Size: 35 787
SacII CC_GC'GG
Cuts at: 0 62 822
Size: 62 760
SalI G'TCGA_C
Cuts at: 0 782 822
Size: 782 40
SapI GCTCTTCn'nnn_
Cuts at: 0 257 822
Size: 257 565
SfcI C'TryA_G
Cuts at: 0 161 218 822
Size: 161 57 604
Fragments arranged by size:
604 161 57
XbaI T'CTAG_A
Cuts at: 0 198 822
Size: 198 624
XmnI GAAnn'nnTTC
Cuts at: 0 541 822
Size: 541 281
Enzymes that do cut:
AccI Acc65I AcuI ApaI ApaLI AseI BanI BanII
BbsI BbvCI BclI BfrBI BglI Bme1580I BpmI Bpu10I
BseRI BseYI BsiHKAI Bsp1286I BsrDI Bsu36I BtgI DraIII
DrdI EaeI EarI EcoICRI Eco57MI HincII KpnI MslI
MspA1I NsiI PfoI PspOMI PstI SacI SacII SalI
SapI SfcI XbaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApoI AscI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BciVI BglII BlpI
BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI BtsI
ClaI CspCI DraI EagI EciI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HindIII
HpaI KasI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XcmI XhoI XmaI ZraI