################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvrII BalI BbrPI BbsI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EarI Eco47III EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI Ksp632I MamI MboII MfeI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AhdI 466 GACGCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 790 CCTGAGG CC^T[ACGT]AGG ApaI 57 GGGCCC GGGCC^C AspEI 466 GACGCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 604 GGATCC G^GATCC BbeI 339 GGCGCC GGCGC^C BcgI 651 CGACCCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 574 GTATCC GTATCC(6/5) BfaI 418 CTAG C^TAG BsaI 754 GAGACC GGTCTC(1/5) BsrI 260 CCAGT ACTGG(1/-1) BssSI 307 CACGAG CACGAG(-5/-1) BstAPI 306 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 118 CCAGACTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 604 GGATCC [GA]^GATC[CT] Bsu36I 790 CCTGAGG CC^T[ACGT]AGG Eam1105I 466 GACGCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 790 CCTGAGG CC^T[ACGT]AGG EcoNI 423 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HphI 463 GGTGA GGTGA(8/7) KasI 339 GGCGCC G^GCGCC KspI 344 CCGCGG CCGC^GG MaeI 418 CTAG C^TAG MaeII 737 ACGT A^CGT MaeIII 464 GTGAC ^GT[ACGT]AC MflI 604 GGATCC [GA]^GATC[CT] NaeI 452 GCCGGC GCC^GGC NarI 339 GGCGCC GG^CGCC NgoMIV 452 GCCGGC G^CCGGC NspI 281 GCATGC [GA]CATG^[CT] SacII 344 CCGCGG CCGC^GG SfoI 339 GGCGCC GGC^GCC SgrAI 445 CGCCGGTG C[GA]^CCGG[CT]G SphI 281 GCATGC GCATG^C StuI 420 AGGCCT AGG^CCT TaqI 368 TCGA T^CGA Tsp45I 464 GTGAC ^GT[GC]AC TspRI 261 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 368 TCGA T^CGA XhoII 604 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 339 GGCGCC G[GA]^CG[CT]C 594 GGCGTC AluI 311 AGCT AG^CT 633 AGCT AlwI 605 GATCC GGATC(4/5) 604 GGATC AlwNI 7 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 352 CAGAGCCTG AvaII 186 GGACC G^G[AT]CC 473 GGTCC AviII 278 TGCGCA TGC^GCA 284 TGCGCA BbvCI 423 CCTCAGC CCTCAGC(-5/-2) 538 GCTGAGG BglI 230 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 670 GCCGAGGCGGC BpmI 439 CTCCAG CTGGAG(16/14) 778 CTGGAG BsaHI 339 GGCGCC G[GA]^CG[CT]C 594 GGCGTC BseRI 430 GAGGAG GAGGAG(10/8) 793 GAGGAG BsgI 304 CTGCAC GTGCAG(16/14) 635 CTGCAC BsmBI 108 CGTCTC CGTCTC(1/5) 370 GAGACG BsrFI 446 GCCGGT [GA]^CCGG[CT] 452 GCCGGC BssHII 218 GCGCGC G^CGCGC 220 GCGCGC BstF5I 38 GGATG GGATG(2/0) 761 GGATG Cfr10I 446 GCCGGT [GA]^CCGG[CT] 452 GCCGGC DpnII 366 GATC ^GATC 605 GATC FokI 38 GGATG GGATG(9/13) 761 GGATG FspI 278 TGCGCA TGC^GCA 284 TGCGCA Hin1I 339 GGCGCC G[GA]^CG[CT]C 594 GGCGTC MboI 366 GATC ^GATC 605 GATC NdeII 366 GATC ^GATC 605 GATC NlaIII 125 CATG CATG^ 282 CATG NotI 69 GCGGCCGC GC^GGCCGC 95 GCGGCCGC PpuMI 185 GGGACCC [GA]G^G[AT]CC[CT] 472 GGGTCCC Sau3AI 366 GATC ^GATC 605 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AccII AciI AvaI BanI BanII BbvI BcnI BmyI BsaJI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV PleI Sau96I ScrFI SfaNI TseI