(Circular) (Six-base) MAPSORT of: pFN21AE3677.seq Check: 5801 from: 1 to: 5509 pFN21AE3677 5509 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3319 278 Size: 53 83 186 936 1783 2468 Fragments arranged by size: 2468 1783 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2852 1937 Size: 915 4594 Acc65I G'GTAC_C Cuts at: 2830 2830 Size: 5509 AclI AA'CG_TT Cuts at: 1563 3636 4009 1563 Size: 2073 373 3063 Fragments arranged by size: 3063 2073 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3572 4956 5224 1632 Size: 1940 1384 268 1917 Fragments arranged by size: 1940 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5312 AflIII A'CryG_T Cuts at: 1203 4408 5385 1203 Size: 3205 977 1327 Fragments arranged by size: 3205 1327 977 AgeI A'CCGG_T Cuts at: 4340 4340 Size: 5509 AhdI GACnn_n'nnGTC Cuts at: 2476 4238 2476 Size: 1762 3747 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5509 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5509 AlwNI CAG_nnn'CTG Cuts at: 2017 2362 3252 4824 2017 Size: 345 890 1572 2702 Fragments arranged by size: 2702 1572 890 345 ApaI G_GGCC'C Cuts at: 2066 2066 Size: 5509 ApaLI G'TGCA_C Cuts at: 3566 4722 3566 Size: 1156 4353 ApoI r'AATT_y Cuts at: 1493 2818 3044 3196 5305 1493 Size: 1325 226 152 2109 1697 Fragments arranged by size: 2109 1697 1325 226 152 AseI AT'TA_AT Cuts at: 160 1032 4063 160 Size: 872 3031 1606 Fragments arranged by size: 3031 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 AvaI C'yCGr_G Cuts at: 1870 1943 2484 2634 2645 2834 1870 Size: 73 541 150 11 189 4545 Fragments arranged by size: 4545 541 189 150 73 11 BamHI G'GATC_C Cuts at: 2609 2839 3206 2609 Size: 230 367 4912 Fragments arranged by size: 4912 367 230 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2180 2344 2454 2830 4285 Size: 325 217 627 393 164 110 376 1455 Cuts at: 4285 618 Size: 1842 Fragments arranged by size: 1842 1455 627 393 376 325 217 164 110 BanII G_rGCy'C Cuts at: 1391 2066 2168 2635 2805 2828 1391 Size: 675 102 467 170 23 4072 Fragments arranged by size: 4072 675 467 170 102 23 BbeI G_GCGC'C Cuts at: 2348 2348 Size: 5509 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4538 BbvCI CC'TCA_GC Cuts at: 2429 2544 2429 Size: 115 5394 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2645 2679 3700 3734 1903 Size: 34 708 34 1021 34 3678 Fragments arranged by size: 3678 1021 708 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2590 3401 4611 2590 Size: 811 1210 3488 Fragments arranged by size: 3488 1210 811 BclI T'GATC_A Cuts at: 1548 1548 Size: 5509 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2241 2681 4120 Size: 107 122 71 631 605 569 440 1439 Cuts at: 4120 136 Size: 1525 Fragments arranged by size: 1525 1439 631 605 569 440 122 107 71 BglII A'GATC_T Cuts at: 5504 5504 Size: 5509 BlpI GC'TnA_GC Cuts at: 2926 2926 Size: 5509 Bme1580I G_kGCm'C Cuts at: 1790 2024 2066 2504 2690 3570 4726 1790 Size: 234 42 438 186 880 1156 2573 Fragments arranged by size: 2573 1156 880 438 234 186 42 BmgBI CAC'GTC Cuts at: 1318 2743 1318 Size: 1425 4084 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3240 4198 442 Size: 416 53 83 399 24 1823 958 1753 Fragments arranged by size: 1823 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5509 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2429 2804 4169 1239 Size: 1190 375 1365 2579 Fragments arranged by size: 2579 1365 1190 375 Bpu10I CC'TnA_GC Cuts at: 2429 2544 2429 Size: 115 5394 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3634 4499 4797 3634 Size: 865 298 4346 Fragments arranged by size: 4346 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2753 4172 882 Size: 617 1254 1419 2219 Fragments arranged by size: 2219 1419 1254 617 BsaAI yAC'GTr Cuts at: 493 1204 3300 493 Size: 711 2096 2702 Fragments arranged by size: 2702 2096 711 BsaBI GATnn'nnATC Cuts at: 5503 5503 Size: 5509 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2345 2600 3316 3698 Size: 53 83 186 936 812 255 716 382 Cuts at: 3698 4333 275 Size: 635 1451 Fragments arranged by size: 1451 936 812 716 635 382 255 186 83 53 BsaWI w'CCGG_w Cuts at: 3942 4340 4614 4761 5172 3942 Size: 398 274 147 411 4279 Fragments arranged by size: 4279 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5284 Fragments arranged by size: 5284 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2450 2813 1186 Size: 1264 363 3882 Fragments arranged by size: 3882 1264 363 BseYI C'CCAG_C Cuts at: 2010 2143 2569 4712 5208 2010 Size: 133 426 2143 496 2311 Fragments arranged by size: 2311 2143 496 426 133 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2294 2625 1163 Size: 573 558 331 4047 Fragments arranged by size: 4047 573 558 331 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2078 2104 2418 2959 3720 4748 Size: 275 186 77 26 314 541 761 1028 Cuts at: 4748 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 541 314 275 186 77 26 BsiHKAI G_wGCw'C Cuts at: 3570 3655 4726 3570 Size: 85 1071 4353 Fragments arranged by size: 4353 1071 85 BsmI GAATG_Cn' Cuts at: 3020 3113 3020 Size: 93 5416 BsmBI CGTCTCn'nnnn_ Cuts at: 2119 2369 2119 Size: 250 5259 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2066 2168 2504 2635 2690 2805 Size: 399 234 42 102 336 131 55 115 Cuts at: 2805 2828 3570 3655 4726 1391 Size: 23 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 336 234 131 115 102 85 55 42 23 BspEI T'CCGG_A Cuts at: 5172 5172 Size: 5509 BspHI T'CATG_A Cuts at: 3398 5128 3398 Size: 1730 3779 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2864 844 Size: 2020 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2029 2106 2272 2728 3396 1394 Size: 429 206 77 166 456 668 3507 Fragments arranged by size: 3507 668 456 429 206 166 77 BsrDI GCAATG_nn' Cuts at: 65 1248 4004 4178 65 Size: 1183 2756 174 1396 Fragments arranged by size: 2756 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2451 2457 4153 4340 1700 Size: 110 641 6 1696 187 2869 Fragments arranged by size: 2869 1696 641 187 110 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5509 BssHII G'CGCG_C Cuts at: 1925 2223 2225 1925 Size: 298 2 5209 Fragments arranged by size: 5209 298 2 BssSI C'ACGA_G Cuts at: 2312 3569 4581 2312 Size: 1257 1012 3240 Fragments arranged by size: 3240 1257 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2317 794 Size: 1523 3986 BstBI TT'CG_AA Cuts at: 4313 4313 Size: 5509 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5509 BstXI CCAn_nnnn'nTGG Cuts at: 2130 2130 Size: 5509 BstYI r'GATC_y Cuts at: 1972 2609 2839 3206 3591 3608 5060 5146 5158 Size: 637 230 367 385 17 1452 86 12 Cuts at: 5158 5504 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 637 385 367 346 230 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2796 2796 Size: 5509 BtgI C'CryG_G Cuts at: 513 1065 2004 2157 2340 2349 513 Size: 552 939 153 183 9 3673 Fragments arranged by size: 3673 939 552 183 153 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2717 487 Size: 683 56 1491 3279 Fragments arranged by size: 3279 1491 683 56 BtsI GCAGTG_nn' Cuts at: 3025 3819 3839 3025 Size: 794 20 4695 Fragments arranged by size: 4695 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5509 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5474 DraI TTT'AAA Cuts at: 2813 2813 Size: 5509 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4516 809 Size: 507 3200 1802 Fragments arranged by size: 3200 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2075 2101 2174 Size: 54 1425 50 275 18 245 26 73 Cuts at: 2174 2404 2415 2956 3845 5474 8 Size: 230 11 541 889 1629 43 Fragments arranged by size: 1629 1425 889 541 275 245 230 73 54 50 43 26 18 11 EagI C'GGCC_G Cuts at: 1537 1812 2075 2101 2415 2956 1537 Size: 275 263 26 314 541 4090 Fragments arranged by size: 4090 541 314 275 263 26 EarI CTCTTCn'nnn_ Cuts at: 1353 3439 1353 Size: 2086 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2572 4096 4470 4616 387 Size: 1064 138 983 1524 374 146 1280 Fragments arranged by size: 1524 1280 1064 983 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2429 2804 3572 4169 4956 5224 1239 Size: 393 797 375 768 597 787 268 1524 Fragments arranged by size: 1524 797 787 768 597 393 375 268 EcoNI CCTnn'n_nnAGG Cuts at: 2432 2432 Size: 5509 EcoO109I rG'GnC_Cy Cuts at: 2062 2191 2478 2062 Size: 129 287 5093 Fragments arranged by size: 5093 287 129 EcoRI G'AATT_C Cuts at: 2818 3196 2818 Size: 378 5131 FspI TGC'GCA Cuts at: 2285 2291 4015 2285 Size: 6 1724 3779 Fragments arranged by size: 3779 1724 6 FspAI rTGC'GCAy Cuts at: 2291 2291 Size: 5509 HaeII r_GCGC'y Cuts at: 1678 2265 2348 2452 4656 1678 Size: 587 83 104 2204 2531 Fragments arranged by size: 2531 2204 587 104 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2462 2494 4150 4182 4224 Size: 32 135 32 890 32 1656 32 42 Cuts at: 4224 4256 1373 Size: 32 2626 Fragments arranged by size: 2626 1656 890 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2853 3099 669 Size: 988 281 915 246 3079 Fragments arranged by size: 3079 988 915 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5509 HpaI GTT'AAC Cuts at: 3099 3099 Size: 5509 KasI G'GCGC_C Cuts at: 2344 2344 Size: 5509 KpnI G_GTAC'C Cuts at: 2834 2834 Size: 5509 MfeI C'AATT_G Cuts at: 3108 3108 Size: 5509 MluI A'CGCG_T Cuts at: 5385 5385 Size: 5509 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2112 2736 4623 4807 1305 Size: 807 624 1887 184 2007 Fragments arranged by size: 2007 1887 807 624 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3687 Fragments arranged by size: 3687 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3467 3826 3985 518 Size: 648 2301 359 159 2042 Fragments arranged by size: 2301 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2247 2351 2497 2925 3602 4750 4995 1606 Size: 641 104 146 428 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 641 428 245 146 104 NaeI GCC'GGC Cuts at: 2459 2459 Size: 5509 NarI GG'CG_CC Cuts at: 2345 2345 Size: 5509 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4018 Fragments arranged by size: 4018 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5509 NgoMIV G'CCGG_C Cuts at: 2457 2457 Size: 5509 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5509 NotI GC'GGCC_GC Cuts at: 2075 2101 2956 2075 Size: 26 855 4628 Fragments arranged by size: 4628 855 26 NspI r_CATG'y Cuts at: 1132 1229 2290 2867 4412 1132 Size: 97 1061 577 1545 2229 Fragments arranged by size: 2229 1545 1061 577 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5509 PciI A'CATG_T Cuts at: 4408 4408 Size: 5509 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5509 PfoI T'CCnGG_A Cuts at: 2039 2187 2039 Size: 148 5361 PmeI GTTT'AAAC Cuts at: 2813 2813 Size: 5509 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3578 3610 5091 5123 3578 Size: 32 1481 32 3964 Fragments arranged by size: 3964 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2191 2478 2191 Size: 287 5222 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5509 PsiI TTA'TAA Cuts at: 3079 3079 Size: 5509 PspOMI G'GGCC_C Cuts at: 2062 2062 Size: 5509 PstI C_TGCA'G Cuts at: 830 2861 830 Size: 2031 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5509 SacII CC_GC'GG Cuts at: 2352 2352 Size: 5509 SalI G'TCGA_C Cuts at: 1936 2851 1936 Size: 915 4594 SanDI GG'GwC_CC Cuts at: 2191 2478 2191 Size: 287 5222 SbfI CC_TGCA'GG Cuts at: 2861 2861 Size: 5509 ScaI AGT'ACT Cuts at: 3757 5469 3757 Size: 1712 3797 SfcI C'TryA_G Cuts at: 826 1045 2857 3992 4673 4864 826 Size: 219 1812 1135 681 191 1471 Fragments arranged by size: 1812 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2346 2346 Size: 5509 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 SgrAI Cr'CCGG_yG Cuts at: 1700 2451 1700 Size: 751 4758 SmaI CCC'GGG Cuts at: 1872 2836 1872 Size: 964 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3613 4514 4776 820 Size: 197 926 1670 901 262 1553 Fragments arranged by size: 1670 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3300 493 Size: 2807 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5509 SphI G_CATG'C Cuts at: 1132 2290 2867 1132 Size: 1158 577 3774 Fragments arranged by size: 3774 1158 577 SspI AAT'ATT Cuts at: 5 52 3433 5 Size: 47 3381 2081 Fragments arranged by size: 3381 2081 47 StuI AGG'CCT Cuts at: 1517 2427 1517 Size: 910 4599 StyI C'CwwG_G Cuts at: 513 1065 2004 2805 2948 513 Size: 552 939 801 143 3074 Fragments arranged by size: 3074 939 801 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3536 3553 3706 3891 4336 325 Size: 1255 1956 17 153 185 445 1498 Fragments arranged by size: 1956 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3755 5467 96 Size: 274 80 33 51 1443 1778 1712 138 Fragments arranged by size: 1778 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5469 5501 5469 Size: 32 5477 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5509 XbaI T'CTAG_A Cuts at: 2845 2845 Size: 5509 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5509 XmaI C'CCGG_G Cuts at: 1870 2834 1870 Size: 964 4545 XmnI GAAnn'nnTTC Cuts at: 3638 3638 Size: 5509 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3317 276 Size: 53 83 186 936 1783 2468 Fragments arranged by size: 2468 1783 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI XcmI