Restriction map for
pFN21AE3677
(Linear) (Six-base) MAPSORT of: KIEE3677.seq Check: 5498 from: 1 to: 804
KIEE3677.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 471 804
Size: 471 333
AlwNI CAG_nnn'CTG
Cuts at: 0 12 357 804
Size: 12 345 447
Fragments arranged by size:
447 345 12
ApaI G_GGCC'C
Cuts at: 0 61 804
Size: 61 743
AvaI C'yCGr_G
Cuts at: 0 479 629 640 804
Size: 479 150 11 164
Fragments arranged by size:
479 164 150 11
BamHI G'GATC_C
Cuts at: 0 604 804
Size: 604 200
BanI G'GyrC_C
Cuts at: 0 175 339 449 804
Size: 175 164 110 355
Fragments arranged by size:
355 175 164 110
BanII G_rGCy'C
Cuts at: 0 61 163 630 800 804
Size: 61 102 467 170 4
Fragments arranged by size:
467 170 102 61 4
BbeI G_GCGC'C
Cuts at: 0 343 804
Size: 343 461
BbvCI CC'TCA_GC
Cuts at: 0 424 539 804
Size: 424 115 265
Fragments arranged by size:
424 265 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 640 674 804
Size: 640 34 130
Fragments arranged by size:
640 130 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 585 804
Size: 585 219
BglI GCCn_nnn'nGGC
Cuts at: 0 236 676 804
Size: 236 440 128
Fragments arranged by size:
440 236 128
Bme1580I G_kGCm'C
Cuts at: 0 19 61 499 685 804
Size: 19 42 438 186 119
Fragments arranged by size:
438 186 119 42 19
BmgBI CAC'GTC
Cuts at: 0 738 804
Size: 738 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 799 804
Size: 424 375 5
Fragments arranged by size:
424 375 5
Bpu10I CC'TnA_GC
Cuts at: 0 424 539 804
Size: 424 115 265
Fragments arranged by size:
424 265 115
BsaI GGTCTCn'nnnn_
Cuts at: 0 748 804
Size: 748 56
BsaHI Gr'CG_yC
Cuts at: 0 340 595 804
Size: 340 255 209
Fragments arranged by size:
340 255 209
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 445 804
Size: 445 359
BseYI C'CCAG_C
Cuts at: 0 5 138 564 804
Size: 5 133 426 240
Fragments arranged by size:
426 240 133 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 620 804
Size: 289 331 184
Fragments arranged by size:
331 289 184
BsiEI CG_ry'CG
Cuts at: 0 73 99 413 804
Size: 73 26 314 391
Fragments arranged by size:
391 314 73 26
BsmBI CGTCTCn'nnnn_
Cuts at: 0 114 364 804
Size: 114 250 440
Fragments arranged by size:
440 250 114
Bsp1286I G_dGCh'C
Cuts at: 0 19 61 163 499 630 685 800 804
Size: 19 42 102 336 131 55 115 4
Fragments arranged by size:
336 131 115 102 55 42 19 4
BsrBI CCG'CTC
Cuts at: 0 24 101 267 723 804
Size: 24 77 166 456 81
Fragments arranged by size:
456 166 81 77 24
BsrFI r'CCGG_y
Cuts at: 0 446 452 804
Size: 446 6 352
Fragments arranged by size:
446 352 6
BssHII G'CGCG_C
Cuts at: 0 218 220 804
Size: 218 2 584
Fragments arranged by size:
584 218 2
BssSI C'ACGA_G
Cuts at: 0 307 804
Size: 307 497
BstAPI GCAn_nnn'nTGC
Cuts at: 0 312 804
Size: 312 492
BstXI CCAn_nnnn'nTGG
Cuts at: 0 125 804
Size: 125 679
BstYI r'GATC_y
Cuts at: 0 604 804
Size: 604 200
Bsu36I CC'TnA_GG
Cuts at: 0 791 804
Size: 791 13
BtgI C'CryG_G
Cuts at: 0 152 335 344 804
Size: 152 183 9 460
Fragments arranged by size:
460 183 152 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 712 804
Size: 712 92
EaeI y'GGCC_r
Cuts at: 0 70 96 169 399 410 804
Size: 70 26 73 230 11 394
Fragments arranged by size:
394 230 73 70 26 11
EagI C'GGCC_G
Cuts at: 0 70 96 410 804
Size: 70 26 314 394
Fragments arranged by size:
394 314 70 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 567 804
Size: 567 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 799 804
Size: 424 375 5
Fragments arranged by size:
424 375 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 427 804
Size: 427 377
EcoO109I rG'GnC_Cy
Cuts at: 0 57 186 473 804
Size: 57 129 287 331
Fragments arranged by size:
331 287 129 57
FspI TGC'GCA
Cuts at: 0 280 286 804
Size: 280 6 518
Fragments arranged by size:
518 280 6
FspAI rTGC'GCAy
Cuts at: 0 286 804
Size: 286 518
HaeII r_GCGC'y
Cuts at: 0 260 343 447 804
Size: 260 83 104 357
Fragments arranged by size:
357 260 104 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 457 489 804
Size: 457 32 315
Fragments arranged by size:
457 315 32
KasI G'GCGC_C
Cuts at: 0 339 804
Size: 339 465
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 731 804
Size: 107 624 73
Fragments arranged by size:
624 107 73
MspA1I CmG'CkG
Cuts at: 0 242 346 492 804
Size: 242 104 146 312
Fragments arranged by size:
312 242 146 104
NaeI GCC'GGC
Cuts at: 0 454 804
Size: 454 350
NarI GG'CG_CC
Cuts at: 0 340 804
Size: 340 464
NgoMIV G'CCGG_C
Cuts at: 0 452 804
Size: 452 352
NotI GC'GGCC_GC
Cuts at: 0 70 96 804
Size: 70 26 708
Fragments arranged by size:
708 70 26
NspI r_CATG'y
Cuts at: 0 285 804
Size: 285 519
PfoI T'CCnGG_A
Cuts at: 0 34 182 804
Size: 34 148 622
Fragments arranged by size:
622 148 34
PpuMI rG'GwC_Cy
Cuts at: 0 186 473 804
Size: 186 287 331
Fragments arranged by size:
331 287 186
PspOMI G'GGCC_C
Cuts at: 0 57 804
Size: 57 747
SacII CC_GC'GG
Cuts at: 0 347 804
Size: 347 457
SanDI GG'GwC_CC
Cuts at: 0 186 473 804
Size: 186 287 331
Fragments arranged by size:
331 287 186
SfoI GGC'GCC
Cuts at: 0 341 804
Size: 341 463
SgrAI Cr'CCGG_yG
Cuts at: 0 446 804
Size: 446 358
SphI G_CATG'C
Cuts at: 0 285 804
Size: 285 519
StuI AGG'CCT
Cuts at: 0 422 804
Size: 422 382
Enzymes that do cut:
AhdI AlwNI ApaI AvaI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BglI Bme1580I BmgBI BpmI Bpu10I
BsaI BsaHI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I
BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI EaeI EagI EciI Eco57MI EcoNI EcoO109I
FspI FspAI HaeII Hin4I KasI MmeI MspA1I NaeI
NarI NgoMIV NotI NspI PfoI PpuMI PspOMI SacII
SanDI SfoI SgrAI SphI StuI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbsI BclI BfrBI BglII
BlpI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaWI
BsaXI BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrDI
BsrGI BstBI BstEII BstZ17I BtsI ClaI CspCI DraI
DraIII DrdI EarI EcoICRI EcoRI EcoRV FalI FseI
HincII HindIII HpaI KpnI MfeI MluI MscI MslI
NcoI NdeI NheI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SmaI SmlI SnaBI
SpeI SrfI SspI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI