Restriction map for
pFN21AE2483
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AvrII BanII BbeI BbvCI
BcgI BciVI BclI BfaI BlnI
BlpI BmyI Bpu1102I BsaBI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT14I EcoT22I FbaI
FseI HaeII HindIII HinfI HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TfiI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcsI 502 GAATTC [GA]^AATT[CT]
AcyI 921 GACGCC G[GA]^CG[CT]C
AflII 172 CTTAAG C^TTAAG
AlwNI 859 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 502 GAATTC [GA]^AATT[CT]
Asp700I 465 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 303 GGTCC G^G[AT]CC
AviII 944 TGCGCA TGC^GCA
BalI 446 TGGCCA TGG^CCA
BamHI 380 GGATCC G^GATCC
BanI 433 GGTGCC G^G[CT][GA]CC
BbrPI 165 CACGTG CAC^GTG
BbsI 145 GTCTTC GAAGAC(2/6)
BcnI 306 CCCGG CC^[GC]GG
BfrI 172 CTTAAG C^TTAAG
BglI 170 GCCTTAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 869 AGATCT A^GATCT
BpuAI 145 GTCTTC GAAGAC(2/6)
BsaHI 921 GACGCC G[GA]^CG[CT]C
BsaI 589 GAGACC GGTCTC(1/5)
BspHI 453 TCATGA T^CATGA
BssHII 35 GCGCGC G^CGCGC
BssSI 627 CACGAG CACGAG(-5/-1)
DdeI 605 CTCAG C^T[ACGT]AG
DraII 302 GGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 775 CTCTTC CTCTTC(1/4)
EcoO109I 302 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 502 GAATTC G^AATTC
EcoRV 73 GATATC GAT^ATC
FspI 944 TGCGCA TGC^GCA
HapII 307 CCGG C^CGG
Hin1I 921 GACGCC G[GA]^CG[CT]C
HincII 577 GTCAAC GT[CT]^[GA]AC
HindII 577 GTCAAC GT[CT]^[GA]AC
HpaII 307 CCGG C^CGG
Ksp632I 775 CTCTTC CTCTTC(1/4)
MluNI 446 TGGCCA TGG^CCA
MscI 446 TGGCCA TGG^CCA
MslI 97 CACAACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 780 CAGCTG C[AC]G^C[GT]G
MspI 307 CCGG C^CGG
NciI 306 CCCGG CC^[GC]GG
NspI 573 GCATGT [GA]CATG^[CT]
PmaCI 165 CACGTG CAC^GTG
PmlI 165 CACGTG CAC^GTG
PpuMI 302 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 865 CTGCAG CTGCA^G
PvuII 780 CAGCTG CAG^CTG
RcaI 453 TCATGA T^CATGA
SmlI 172 CTTAAG C^T[CT][GA]AG
StuI 467 AGGCCT AGG^CCT
Tsp509I 503 AATT ^AATT
XmnI 465 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
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AlwI 381 GATCC GGATC(4/5)
380 GGATC
BpmI 744 CTCCAG CTGGAG(16/14)
988 CTGGAG
BsaAI 165 CACGTG [CT]AC^GT[GA]
610 TACGTG
BsgI 162 CTGCAC GTGCAG(16/14)
645 CTGCAC
BsmBI 199 GAGACG CGTCTC(1/5)
919 GAGACG
BsmFI 887 GGGAC GGGAC(10/14)
304 GTCCC
BspMI 160 ACCTGC ACCTGC(4/8)
392 ACCTGC
BsrDI 240 CATTGC GCAATG(2/0)
362 GCAATG
BstYI 380 GGATCC [GA]^GATC[CT]
869 AGATCT
EaeI 20 TGGCCG [CT]^GGCC[GA]
446 TGGCCA
HgaI 426 GACGC GACGC(5/10)
921 GACGC
HphI 387 GGTGA GGTGA(8/7)
423 GGTGA
MaeII 166 ACGT A^CGT
611 ACGT
MflI 380 GGATCC [GA]^GATC[CT]
869 AGATCT
TaqI 212 TCGA T^CGA
506 TCGA
Tsp45I 277 GTGAC ^GT[GC]AC
424 GTGAC
TthHB8I 212 TCGA T^CGA
506 TCGA
XhoII 380 GGATCC [GA]^GATC[CT]
869 AGATCT
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AfaI AluI BbvI
BsaJI BseRI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HhaI HinP1I ItaI MaeIII MboI
MboII MnlI MseI MvaI MvnI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
Tru9I TseI TspRI