(Circular) (Six-base) MAPSORT of: pFN21AE2483.seq Check: 763 from: 1 to: 5710 pFN21AE2483 5710 bp With 182 enzymes: SgfI * September 20, 2013 17:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2174 2174 Size: 5710 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3053 1937 Size: 1116 4594 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2489 2732 2767 3773 5157 5425 1632 Size: 857 243 35 1006 1384 268 1917 Fragments arranged by size: 1917 1384 1006 857 268 243 35 AflII C'TTAA_G Cuts at: 820 1017 2177 820 Size: 197 1160 4353 Fragments arranged by size: 4353 1160 197 AflIII A'CryG_T Cuts at: 1203 4609 5586 1203 Size: 3406 977 1327 Fragments arranged by size: 3406 1327 977 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 4439 4439 Size: 5710 AleI CACnn'nnGTG Cuts at: 1166 2106 1166 Size: 940 4770 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3002 1184 Size: 1818 3892 AlwNI CAG_nnn'CTG Cuts at: 2869 3453 5025 2869 Size: 584 1572 3554 Fragments arranged by size: 3554 1572 584 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 2507 3019 3245 3397 5506 1493 Size: 1014 512 226 152 2109 1697 Fragments arranged by size: 2109 1697 1014 512 226 152 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 3035 1870 Size: 73 1092 4545 Fragments arranged by size: 4545 1092 73 BamHI G'GATC_C Cuts at: 2385 3040 3407 2385 Size: 655 367 4688 Fragments arranged by size: 4688 655 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2438 3031 4486 618 Size: 325 217 627 651 593 1455 1842 Fragments arranged by size: 1842 1455 651 627 593 325 217 BanII G_rGCy'C Cuts at: 1391 3029 1391 Size: 1638 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2143 928 Size: 971 244 4495 Fragments arranged by size: 4495 971 244 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3901 3935 1903 Size: 34 50 34 1880 34 3678 Fragments arranged by size: 3678 1880 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2181 4321 136 Size: 107 122 71 631 605 509 2140 1525 Fragments arranged by size: 2140 1525 631 605 509 122 107 71 BglII A'GATC_T Cuts at: 2874 5705 2874 Size: 2831 2879 BlpI GC'TnA_GC Cuts at: 3127 3127 Size: 5710 Bme1580I G_kGCm'C Cuts at: 1790 3771 4927 1790 Size: 1981 1156 2573 Fragments arranged by size: 2573 1981 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2234 2681 2899 Size: 416 53 83 399 24 817 447 218 Cuts at: 2899 3441 4399 442 Size: 542 958 1753 Fragments arranged by size: 1753 958 817 542 447 416 399 218 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2734 3014 4370 1239 Size: 1495 280 1356 2579 Fragments arranged by size: 2579 1495 1356 280 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3835 4700 4998 3835 Size: 865 298 4547 Fragments arranged by size: 4547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2588 4373 882 Size: 617 1089 1785 2219 Fragments arranged by size: 2219 1785 1089 617 BsaAI yAC'GTr Cuts at: 493 1204 2172 2617 3501 493 Size: 711 968 445 884 2702 Fragments arranged by size: 2702 968 884 711 445 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2927 3517 3899 4534 Size: 53 83 186 936 1394 590 382 635 Cuts at: 4534 275 Size: 1451 Fragments arranged by size: 1451 1394 936 635 590 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4143 4541 4815 4962 5373 4143 Size: 398 274 147 411 4480 Fragments arranged by size: 4480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2597 2627 2839 2869 2885 Size: 30 165 30 1194 30 212 30 16 Cuts at: 2885 2915 1178 Size: 30 3973 Fragments arranged by size: 3973 1194 212 165 30 30 30 30 30 16 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2135 2157 1186 Size: 868 81 22 4739 Fragments arranged by size: 4739 868 81 22 BseYI C'CCAG_C Cuts at: 2057 2360 3001 4913 5409 2057 Size: 303 641 1912 496 2358 Fragments arranged by size: 2358 1912 641 496 303 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2152 2635 1163 Size: 573 416 483 4238 Fragments arranged by size: 4238 573 483 416 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3921 4949 1540 Size: 275 186 1159 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3771 3856 4927 3771 Size: 85 1071 4554 Fragments arranged by size: 4554 1071 85 BsmI GAATG_Cn' Cuts at: 3221 3314 3221 Size: 93 5617 BsmBI CGTCTCn'nnnn_ Cuts at: 2198 2918 2198 Size: 720 4990 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3029 3771 3856 4927 1391 Size: 399 1239 742 85 1071 2174 Fragments arranged by size: 2174 1239 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 2458 3599 5329 2458 Size: 1141 1730 2839 Fragments arranged by size: 2839 1730 1141 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2174 2406 3065 844 Size: 1330 232 659 3489 Fragments arranged by size: 3489 1330 659 232 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2244 2374 4205 4379 65 Size: 1183 996 130 1831 174 1396 Fragments arranged by size: 1831 1396 1183 996 174 130 BsrFI r'CCGG_y Cuts at: 1700 1810 4354 4541 1700 Size: 110 2544 187 2869 Fragments arranged by size: 2869 2544 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 2040 1925 Size: 115 5595 BssSI C'ACGA_G Cuts at: 2632 3770 4782 2632 Size: 1138 1012 3560 Fragments arranged by size: 3560 1138 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5710 BstXI CCAn_nnnn'nTGG Cuts at: 2108 2449 2704 2108 Size: 341 255 5114 Fragments arranged by size: 5114 341 255 BstYI r'GATC_y Cuts at: 1972 2385 2874 3040 3407 3792 3809 5261 5347 Size: 413 489 166 367 385 17 1452 86 Cuts at: 5347 5359 5705 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 489 413 385 367 346 166 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5158 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2965 487 Size: 683 56 1739 3232 Fragments arranged by size: 3232 1739 683 56 BtsI GCAGTG_nn' Cuts at: 3226 4020 4040 3226 Size: 794 20 4896 Fragments arranged by size: 4896 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 3014 3014 Size: 5710 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4717 809 Size: 507 3401 1802 Fragments arranged by size: 3401 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2451 3157 Size: 54 1425 50 275 18 195 426 706 Cuts at: 3157 4046 5675 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 706 426 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 1537 Size: 275 1345 4090 Fragments arranged by size: 4090 1345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2786 3640 1353 Size: 1433 854 3423 Fragments arranged by size: 3423 1433 854 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2489 2732 2734 2767 3014 3773 4370 Size: 393 857 243 2 33 247 759 597 Cuts at: 4370 5157 5425 1239 Size: 787 268 1524 Fragments arranged by size: 1524 857 787 759 597 393 268 247 243 33 2 EcoO109I rG'GnC_Cy Cuts at: 2308 2308 Size: 5710 EcoRI G'AATT_C Cuts at: 2507 3019 3397 2507 Size: 512 378 4820 Fragments arranged by size: 4820 512 378 EcoRV GAT'ATC Cuts at: 2080 2080 Size: 5710 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2932 2932 Size: 5710 FspI TGC'GCA Cuts at: 2951 4216 2951 Size: 1265 4445 FspAI rTGC'GCAy Cuts at: 2951 2951 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 4857 1678 Size: 3179 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2133 2165 2885 2917 4351 Size: 32 135 32 561 32 720 32 1434 Cuts at: 4351 4383 4425 4457 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1434 720 561 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2584 3054 3300 669 Size: 988 281 646 470 246 3079 Fragments arranged by size: 3079 988 646 470 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4824 5008 1305 Size: 3519 184 2007 Fragments arranged by size: 3519 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2453 10 Size: 54 1425 343 621 3267 Fragments arranged by size: 3267 1425 621 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2106 3668 4027 4186 518 Size: 648 940 1562 359 159 2042 Fragments arranged by size: 2042 1562 940 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2787 3126 3803 4951 5196 1606 Size: 1181 339 677 1148 245 2120 Fragments arranged by size: 2120 1181 1148 677 339 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5158 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 2582 3068 4613 1132 Size: 97 1353 486 1545 2229 Fragments arranged by size: 2229 1545 1353 486 97 PasI CC'CwG_GG Cuts at: 1348 2704 1348 Size: 1356 4354 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5710 PfoI T'CCnGG_A Cuts at: 2310 2310 Size: 5710 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PmlI CAC'GTG Cuts at: 2172 2172 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2308 2308 Size: 5710 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5710 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PstI C_TGCA'G Cuts at: 830 2874 3062 830 Size: 2044 188 3478 Fragments arranged by size: 3478 2044 188 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 PvuII CAG'CTG Cuts at: 2787 2787 Size: 5710 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SanDI GG'GwC_CC Cuts at: 2308 2308 Size: 5710 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SfcI C'TryA_G Cuts at: 826 1045 2488 2870 2961 3058 4193 4874 5065 Size: 219 1443 382 91 97 1135 681 191 Cuts at: 5065 826 Size: 1471 Fragments arranged by size: 1471 1443 1135 681 382 219 191 97 91 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 3037 1872 Size: 1165 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2177 3814 4715 4977 820 Size: 197 926 234 1637 901 262 1553 Fragments arranged by size: 1637 1553 926 901 262 234 197 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 3068 1132 Size: 1936 3774 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 2474 1517 Size: 957 4753 StyI C'CwwG_G Cuts at: 513 1065 3149 513 Size: 552 2084 3074 Fragments arranged by size: 3074 2084 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2374 3737 3754 3907 4092 4537 325 Size: 1255 794 1363 17 153 185 445 1498 Fragments arranged by size: 1498 1363 1255 794 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3956 5668 96 Size: 274 80 33 51 1443 1979 1712 138 Fragments arranged by size: 1979 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5670 5702 5670 Size: 32 5678 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 3035 1870 Size: 1165 4545 XmnI GAAnn'nnTTC Cuts at: 2474 3839 2474 Size: 1365 4345 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI