Restriction map for
pFN21AE5264
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI BssHII Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MseI MslI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 114 GTAC GT^AC
AflIII 326 ACACGT A^C[GA][CT]GT
AlwI 228 GATCC GGATC(4/5)
AocI 133 CCTGAGG CC^T[ACGT]AGG
ApaI 52 GGGCCC GGGCC^C
AspHI 478 GAGCTC G[AT]GC[AT]^C
AvaI 161 CCCGGG C^[CT]CG[GA]G
AvaII 366 GGACC G^G[AT]CC
BbvCI 181 CCTCAGC CCTCAGC(-5/-2)
BfaI 223 CTAG C^TAG
BsgI 412 CTGCAC GTGCAG(16/14)
BsiHKAI 478 GAGCTC G[AT]GC[AT]^C
BsmAI 382 GTCTC GTCTC(1/5)
BsoBI 161 CCCGGG C^[CT]CG[GA]G
BspHI 264 TCATGA T^CATGA
BsrBI 306 GAGCGG CCGCTC(-3/-3)
BssSI 88 CTCGTG CACGAG(-5/-1)
BstEII 174 GGTCACC G^GT[ACGT]ACC
BstPI 174 GGTCACC G^GT[ACGT]ACC
BstYI 227 AGATCC [GA]^GATC[CT]
Bsu36I 133 CCTGAGG CC^T[ACGT]AGG
CfoI 405 GCGC GCG^C
DraIII 187 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 133 CCTGAGG CC^T[ACGT]AGG
EcoO65I 174 GGTCACC G^GT[ACGT]ACC
EcoT14I 26 CCATGG C^C[AT][AT]GG
HaeII 404 AGCGCC [GA]GCGC^[CT]
HhaI 405 GCGC GCG^C
HinP1I 405 GCGC G^CGC
HinfI 500 GAATC G^A[ACGT]TC
MaeI 223 CTAG C^TAG
MaeIII 175 GTCAC ^GT[ACGT]AC
MboII 457 GAAGA GAAGA(8/7)
MflI 227 AGATCC [GA]^GATC[CT]
MspA1I 168 CAGCGG C[AC]G^C[GT]G
NcoI 26 CCATGG C^CATGG
RcaI 264 TCATGA T^CATGA
RsaI 114 GTAC GT^AC
SacI 478 GAGCTC GAGCT^C
SmaI 161 CCCGGG CCC^GGG
StyI 26 CCATGG C^C[AT][AT]GG
TfiI 500 GAATC G^A[AT]TC
Tsp45I 175 GTCAC ^GT[GC]AC
XcmI 57 CCACCGGGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 227 AGATCC [GA]^GATC[CT]
XmaCI 161 CCCGGG C^CCGGG
XmaI 161 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 235 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
286 CAGGCACTG
BalI 23 TGGCCA TGG^CCA
215 TGGCCA
BstF5I 126 CATCC GGATG(2/0)
423 CATCC
DpnII 144 GATC ^GATC
228 GATC
DraII 51 GGGGCCC [GA]G^G[ACGT]CC[CT]
52 GGGCCCC
EaeI 23 TGGCCA [CT]^GGCC[GA]
215 TGGCCA
EcoO109I 51 GGGGCCC [GA]G^G[ACGT]CC[CT]
52 GGGCCCC
FokI 126 CATCC GGATG(9/13)
423 CATCC
HphI 146 TCACC GGTGA(8/7)
176 TCACC
MaeII 95 ACGT A^CGT
328 ACGT
MboI 144 GATC ^GATC
228 GATC
MluNI 23 TGGCCA TGG^CCA
215 TGGCCA
MscI 23 TGGCCA TGG^CCA
215 TGGCCA
NdeII 144 GATC ^GATC
228 GATC
PstI 232 CTGCAG CTGCA^G
372 CTGCAG
Sau3AI 144 GATC ^GATC
228 GATC
SfaNI 460 GATGC GCATC(5/9)
422 GCATC
SfcI 232 CTGCAG C^T[GA][CT]AG
372 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI Cac8I Cfr13I DdeI
DsaI EcoRII FauI Fnu4HI HaeIII
HapII HpaII ItaI MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
Sau96I ScrFI TseI TspRI