(Circular) (Six-base) MAPSORT of: pFN21AE5264.seq Check: 5029 from: 1 to: 5248 pFN21AE5264 5248 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3058 278 Size: 53 83 186 936 1522 2468 Fragments arranged by size: 2468 1522 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2591 1937 Size: 654 4594 Acc65I G'GTAC_C Cuts at: 2569 2569 Size: 5248 AclI AA'CG_TT Cuts at: 1563 3375 3748 1563 Size: 1812 373 3063 Fragments arranged by size: 3063 1812 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2297 3311 4695 4963 1632 Size: 665 1014 1384 268 1917 Fragments arranged by size: 1917 1384 1014 665 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5051 AflIII A'CryG_T Cuts at: 1203 2331 4147 5124 1203 Size: 1128 1816 977 1327 Fragments arranged by size: 1816 1327 1128 977 AgeI A'CCGG_T Cuts at: 4079 4079 Size: 5248 AhdI GACnn_n'nnGTC Cuts at: 3977 3977 Size: 5248 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5248 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5248 AlwNI CAG_nnn'CTG Cuts at: 2245 2296 2991 4563 2245 Size: 51 695 1572 2930 Fragments arranged by size: 2930 1572 695 51 ApaI G_GGCC'C Cuts at: 2061 2061 Size: 5248 ApaLI G'TGCA_C Cuts at: 3305 4461 3305 Size: 1156 4092 ApoI r'AATT_y Cuts at: 1493 2557 2783 2935 5044 1493 Size: 1064 226 152 2109 1697 Fragments arranged by size: 2109 1697 1064 226 152 AseI AT'TA_AT Cuts at: 160 1032 3802 160 Size: 872 2770 1606 Fragments arranged by size: 2770 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5248 AvaI C'yCGr_G Cuts at: 1870 1943 2166 2573 1870 Size: 73 223 407 4545 Fragments arranged by size: 4545 407 223 73 BamHI G'GATC_C Cuts at: 2578 2945 2578 Size: 367 4881 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2569 4024 618 Size: 325 217 627 782 1455 1842 Fragments arranged by size: 1842 1455 782 627 325 217 BanII G_rGCy'C Cuts at: 1391 2061 2487 2515 2567 1391 Size: 670 426 28 52 4072 Fragments arranged by size: 4072 670 426 52 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4277 BbvCI CC'TCA_GC Cuts at: 2187 2187 Size: 5248 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3439 3473 1903 Size: 34 1502 34 3678 Fragments arranged by size: 3678 1502 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3140 4350 3140 Size: 1210 4038 BclI T'GATC_A Cuts at: 1548 1548 Size: 5248 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3859 136 Size: 107 122 71 631 605 2187 1525 Fragments arranged by size: 2187 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5243 5243 Size: 5248 BlpI GC'TnA_GC Cuts at: 2665 2665 Size: 5248 Bme1580I G_kGCm'C Cuts at: 1790 2061 3309 4465 1790 Size: 271 1248 1156 2573 Fragments arranged by size: 2573 1248 1156 271 BmgBI CAC'GTC Cuts at: 1318 2334 1318 Size: 1016 4232 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2979 3937 442 Size: 416 53 83 399 24 1562 958 1753 Fragments arranged by size: 1753 1562 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5248 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3908 1239 Size: 2669 2579 Bpu10I CC'TnA_GC Cuts at: 2187 2187 Size: 5248 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3373 4238 4536 3373 Size: 865 298 4085 Fragments arranged by size: 4085 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3911 882 Size: 617 2412 2219 Fragments arranged by size: 2412 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3039 493 Size: 711 1835 2702 Fragments arranged by size: 2702 1835 711 BsaBI GATnn'nnATC Cuts at: 5242 5242 Size: 5248 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3055 3437 4072 275 Size: 53 83 186 936 1522 382 635 1451 Fragments arranged by size: 1522 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3681 4079 4353 4500 4911 3681 Size: 398 274 147 411 4018 Fragments arranged by size: 4018 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5023 Fragments arranged by size: 5023 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5248 BseYI C'CCAG_C Cuts at: 2009 2053 4451 4947 2009 Size: 44 2398 496 2310 Fragments arranged by size: 2398 2310 496 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2402 1163 Size: 573 666 4009 Fragments arranged by size: 4009 666 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2698 3459 4487 1540 Size: 275 186 697 761 1028 2301 Fragments arranged by size: 2301 1028 761 697 275 186 BsiHKAI G_wGCw'C Cuts at: 2487 3309 3394 4465 2487 Size: 822 85 1071 3270 Fragments arranged by size: 3270 1071 822 85 BsmI GAATG_Cn' Cuts at: 2759 2852 2759 Size: 93 5155 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2487 2515 2567 3309 3394 4465 Size: 399 271 426 28 52 742 85 1071 Cuts at: 4465 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 426 399 271 85 52 28 BspEI T'CCGG_A Cuts at: 4911 4911 Size: 5248 BspHI T'CATG_A Cuts at: 2269 3137 4867 2269 Size: 868 1730 2650 Fragments arranged by size: 2650 1730 868 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2603 844 Size: 1759 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2313 3135 1394 Size: 429 490 822 3507 Fragments arranged by size: 3507 822 490 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3743 3917 65 Size: 1183 2495 174 1396 Fragments arranged by size: 2495 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3892 4079 1700 Size: 110 2082 187 2869 Fragments arranged by size: 2869 2082 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5248 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5248 BssSI C'ACGA_G Cuts at: 2093 3308 4320 2093 Size: 1215 1012 3021 Fragments arranged by size: 3021 1215 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5248 BstBI TT'CG_AA Cuts at: 4052 4052 Size: 5248 BstEII G'GTnAC_C Cuts at: 1184 2179 1184 Size: 995 4253 BstYI r'GATC_y Cuts at: 1972 2232 2578 2945 3330 3347 4799 4885 4897 Size: 260 346 367 385 17 1452 86 12 Cuts at: 4897 5243 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 346 260 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2139 2139 Size: 5248 BtgI C'CryG_G Cuts at: 513 1065 2004 2031 2104 2217 513 Size: 552 939 27 73 113 3544 Fragments arranged by size: 3544 939 552 113 73 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4509 Fragments arranged by size: 4509 683 56 BtsI GCAGTG_nn' Cuts at: 2374 2764 3558 3578 2374 Size: 390 794 20 4044 Fragments arranged by size: 4044 794 390 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5248 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2194 2229 529 Size: 35 1630 35 3548 Fragments arranged by size: 3548 1630 35 35 DraI TTT'AAA Cuts at: 2552 2552 Size: 5248 DraIII CAC_nnn'GTG Cuts at: 2197 2197 Size: 5248 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4255 809 Size: 507 2939 1802 Fragments arranged by size: 2939 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2220 2695 Size: 54 1425 50 275 18 198 192 475 Cuts at: 2695 3584 5213 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 475 275 198 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2695 1537 Size: 275 883 4090 Fragments arranged by size: 4090 883 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3178 1353 Size: 1825 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3835 4209 4355 387 Size: 1064 138 2246 374 146 1280 Fragments arranged by size: 2246 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2485 2485 Size: 5248 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2297 3311 3908 4695 4963 1239 Size: 393 665 1014 597 787 268 1524 Fragments arranged by size: 1524 1014 787 665 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2057 2058 2057 Size: 1 5247 EcoRI G'AATT_C Cuts at: 2557 2935 2557 Size: 378 4870 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2302 2302 Size: 5248 FspI TGC'GCA Cuts at: 3754 3754 Size: 5248 HaeII r_GCGC'y Cuts at: 1678 2413 4395 1678 Size: 735 1982 2531 Fragments arranged by size: 2531 1982 735 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3889 3921 3963 3995 1373 Size: 32 135 32 2317 32 42 32 2626 Fragments arranged by size: 2626 2317 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2592 2838 669 Size: 988 281 654 246 3079 Fragments arranged by size: 3079 988 654 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5248 HpaI GTT'AAC Cuts at: 2838 2838 Size: 5248 KpnI G_GTAC'C Cuts at: 2573 2573 Size: 5248 MfeI C'AATT_G Cuts at: 2847 2847 Size: 5248 MluI A'CGCG_T Cuts at: 5124 5124 Size: 5248 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4362 4546 1305 Size: 3057 184 2007 Fragments arranged by size: 3057 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2030 2222 10 Size: 54 1425 343 198 192 3036 Fragments arranged by size: 3036 1425 343 198 192 54 MslI CAynn'nnrTG Cuts at: 518 1166 3206 3565 3724 518 Size: 648 2040 359 159 2042 Fragments arranged by size: 2042 2040 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2175 2664 3341 4489 4734 1606 Size: 569 489 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 569 489 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2031 513 Size: 552 939 27 3730 Fragments arranged by size: 3730 939 552 27 NdeI CA'TA_TG Cuts at: 387 387 Size: 5248 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5248 NotI GC'GGCC_GC Cuts at: 2695 2695 Size: 5248 NspI r_CATG'y Cuts at: 1132 1229 2606 4151 1132 Size: 97 1377 1545 2229 Fragments arranged by size: 2229 1545 1377 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5248 PciI A'CATG_T Cuts at: 4147 4147 Size: 5248 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5248 PmeI GTTT'AAAC Cuts at: 2552 2552 Size: 5248 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3317 3349 4830 4862 3317 Size: 32 1481 32 3703 Fragments arranged by size: 3703 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5248 PsiI TTA'TAA Cuts at: 2818 2818 Size: 5248 PspOMI G'GGCC_C Cuts at: 2057 2057 Size: 5248 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2111 2143 2111 Size: 32 5216 PstI C_TGCA'G Cuts at: 830 2241 2381 2600 830 Size: 1411 140 219 3478 Fragments arranged by size: 3478 1411 219 140 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5248 SacI G_AGCT'C Cuts at: 2487 2487 Size: 5248 SalI G'TCGA_C Cuts at: 1936 2590 1936 Size: 654 4594 SbfI CC_TGCA'GG Cuts at: 2600 2600 Size: 5248 ScaI AGT'ACT Cuts at: 3496 5208 3496 Size: 1712 3536 SfcI C'TryA_G Cuts at: 826 1045 2237 2377 2596 3731 4412 4603 826 Size: 219 1192 140 219 1135 681 191 1471 Fragments arranged by size: 1471 1192 1135 681 219 219 191 140 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5248 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5248 SmaI CCC'GGG Cuts at: 1872 2168 2575 1872 Size: 296 407 4545 Fragments arranged by size: 4545 407 296 SmlI C'TyrA_G Cuts at: 820 1017 1943 3352 4253 4515 820 Size: 197 926 1409 901 262 1553 Fragments arranged by size: 1553 1409 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3039 493 Size: 2546 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5248 SphI G_CATG'C Cuts at: 1132 2606 1132 Size: 1474 3774 SspI AAT'ATT Cuts at: 5 52 3172 5 Size: 47 3120 2081 Fragments arranged by size: 3120 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5248 StyI C'CwwG_G Cuts at: 513 1065 2004 2031 2687 513 Size: 552 939 27 656 3074 Fragments arranged by size: 3074 939 656 552 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3275 3292 3445 3630 4075 325 Size: 1255 1695 17 153 185 445 1498 Fragments arranged by size: 1695 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3494 5206 96 Size: 274 80 33 51 1443 1517 1712 138 Fragments arranged by size: 1712 1517 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5208 5240 5208 Size: 32 5216 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5248 XbaI T'CTAG_A Cuts at: 2584 2584 Size: 5248 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2069 2069 Size: 5248 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5248 XmaI C'CCGG_G Cuts at: 1870 2166 2573 1870 Size: 296 407 4545 Fragments arranged by size: 4545 407 296 XmnI GAAnn'nnTTC Cuts at: 3377 3377 Size: 5248 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3056 276 Size: 53 83 186 936 1522 2468 Fragments arranged by size: 2468 1522 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI