Restriction map for
pFN21AE5264
(Linear) (Six-base) MAPSORT of: KIEE5264.seq Check: 8394 from: 1 to: 543
KIEE5264.orf
With 182 enzymes: SgfI *
January 29, 2014 11:53 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 543
Size: 292 251
AflIII A'CryG_T
Cuts at: 0 326 543
Size: 326 217
AlwNI CAG_nnn'CTG
Cuts at: 0 240 291 543
Size: 240 51 252
Fragments arranged by size:
252 240 51
ApaI G_GGCC'C
Cuts at: 0 56 543
Size: 56 487
AvaI C'yCGr_G
Cuts at: 0 161 543
Size: 161 382
BanII G_rGCy'C
Cuts at: 0 56 482 510 543
Size: 56 426 28 33
Fragments arranged by size:
426 56 33 28
BbvCI CC'TCA_GC
Cuts at: 0 182 543
Size: 182 361
Bme1580I G_kGCm'C
Cuts at: 0 56 543
Size: 56 487
BmgBI CAC'GTC
Cuts at: 0 329 543
Size: 329 214
Bpu10I CC'TnA_GC
Cuts at: 0 182 543
Size: 182 361
BseYI C'CCAG_C
Cuts at: 0 4 48 543
Size: 4 44 495
Fragments arranged by size:
495 44 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 543
Size: 397 146
BsiHKAI G_wGCw'C
Cuts at: 0 482 543
Size: 482 61
Bsp1286I G_dGCh'C
Cuts at: 0 56 482 510 543
Size: 56 426 28 33
Fragments arranged by size:
426 56 33 28
BspHI T'CATG_A
Cuts at: 0 264 543
Size: 264 279
BsrBI CCG'CTC
Cuts at: 0 308 543
Size: 308 235
BssSI C'ACGA_G
Cuts at: 0 88 543
Size: 88 455
BstEII G'GTnAC_C
Cuts at: 0 174 543
Size: 174 369
BstYI r'GATC_y
Cuts at: 0 227 543
Size: 227 316
Bsu36I CC'TnA_GG
Cuts at: 0 134 543
Size: 134 409
BtgI C'CryG_G
Cuts at: 0 26 99 212 543
Size: 26 73 113 331
Fragments arranged by size:
331 113 73 26
BtsI GCAGTG_nn'
Cuts at: 0 369 543
Size: 369 174
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 189 224 543
Size: 189 35 319
Fragments arranged by size:
319 189 35
DraIII CAC_nnn'GTG
Cuts at: 0 192 543
Size: 192 351
EaeI y'GGCC_r
Cuts at: 0 23 215 543
Size: 23 192 328
Fragments arranged by size:
328 192 23
EcoICRI GAG'CTC
Cuts at: 0 480 543
Size: 480 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 543
Size: 292 251
EcoO109I rG'GnC_Cy
Cuts at: 0 52 53 543
Size: 52 1 490
Fragments arranged by size:
490 52 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 297 543
Size: 297 246
HaeII r_GCGC'y
Cuts at: 0 408 543
Size: 408 135
MscI TGG'CCA
Cuts at: 0 25 217 543
Size: 25 192 326
Fragments arranged by size:
326 192 25
MspA1I CmG'CkG
Cuts at: 0 170 543
Size: 170 373
NcoI C'CATG_G
Cuts at: 0 26 543
Size: 26 517
PspOMI G'GGCC_C
Cuts at: 0 52 543
Size: 52 491
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 106 138 543
Size: 106 32 405
Fragments arranged by size:
405 106 32
PstI C_TGCA'G
Cuts at: 0 236 376 543
Size: 236 140 167
Fragments arranged by size:
236 167 140
SacI G_AGCT'C
Cuts at: 0 482 543
Size: 482 61
SfcI C'TryA_G
Cuts at: 0 232 372 543
Size: 232 140 171
Fragments arranged by size:
232 171 140
SmaI CCC'GGG
Cuts at: 0 163 543
Size: 163 380
StyI C'CwwG_G
Cuts at: 0 26 543
Size: 26 517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 64 543
Size: 64 479
XmaI C'CCGG_G
Cuts at: 0 161 543
Size: 161 382
Enzymes that do cut:
AcuI AflIII AlwNI ApaI AvaI BanII BbvCI Bme1580I
BmgBI Bpu10I BseYI BsgI BsiHKAI Bsp1286I BspHI BsrBI
BssSI BstEII BstYI Bsu36I BtgI BtsI CspCI DraIII
EaeI EcoICRI Eco57MI EcoO109I FalI HaeII MscI MspA1I
NcoI PspOMI PsrI PstI SacI SfcI SmaI StyI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspMI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstXI BstZ17I
BtgZI ClaI DraI DrdI EagI EarI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmnI ZraI