################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HindIII KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 314 AAATTC [GA]^AATT[CT] AfaI 274 GTAC GT^AC Alw44I 224 GTGCAC G^TGCAC AlwI 99 GATCC GGATC(4/5) ApaLI 224 GTGCAC G^TGCAC ApoI 314 AAATTC [GA]^AATT[CT] AvaI 18 CCCGAG C^[CT]CG[GA]G BanI 54 GGTGCC G^G[CT][GA]CC BbsI 376 GAAGAC GAAGAC(2/6) BlpI 36 GCTGAGC GC^T[ACGT]AGC BpmI 103 CTGGAG CTGGAG(16/14) Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC BpuAI 376 GAAGAC GAAGAC(2/6) BsaI 264 GAGACC GGTCTC(1/5) BsaWI 429 TCCGGT [AT]^CCGG[AT] BsiEI 231 CGGCCG CG[GA][CT]^CG BsmAI 264 GAGAC GTCTC(1/5) BsmFI 213 GGGAC GGGAC(10/14) BsoBI 18 CCCGAG C^[CT]CG[GA]G BsrFI 251 GCCGGC [GA]^CCGG[CT] BsrI 443 CCAGT ACTGG(1/-1) CelII 36 GCTGAGC GC^T[ACGT]AGC CfoI 255 GCGC GCG^C Cfr10I 251 GCCGGC [GA]^CCGG[CT] DpnII 99 GATC ^GATC DraIII 218 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 231 CGGCCG [CT]^GGCC[GA] EagI 231 CGGCCG C^GGCCG EclXI 231 CGGCCG C^GGCCG Eco52I 231 CGGCCG C^GGCCG HaeII 254 GGCGCT [GA]GCGC^[CT] HhaI 255 GCGC GCG^C HinP1I 255 GCGC G^CGC HincII 294 GTTAAC GT[CT]^[GA]AC HindII 294 GTTAAC GT[CT]^[GA]AC HpaI 294 GTTAAC GTT^AAC MboI 99 GATC ^GATC MseI 295 TTAA T^TAA MslI 90 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 251 GCCGGC GCC^GGC NdeII 99 GATC ^GATC NgoMIV 251 GCCGGC G^CCGGC RsaI 274 GTAC GT^AC Sau3AI 99 GATC ^GATC SfaNI 336 GCATC GCATC(5/9) SmlI 59 CTCAAG C^T[CT][GA]AG Tru9I 295 TTAA T^TAA Tsp509I 315 AATT ^AATT XcmI 92 CCACCATGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AvaII 48 GGTCC G^G[AT]CC 214 GGACC BcgI 241 CGAGAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 330 GCAAGAGCATCG BstXI 95 CCATGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 171 CCAGAGTGCTGG EcoT14I 44 CCTTGG C^C[AT][AT]GG 281 CCAAGG HapII 252 CCGG C^CGG 430 CCGG HinfI 112 GACTC G^A[ACGT]TC 154 GAGTC HpaII 252 CCGG C^CGG 430 CCGG MboII 67 GAAGA GAAGA(8/7) 376 GAAGA MspA1I 34 CCGCTG C[AC]G^C[GT]G 362 CAGCGG MspI 252 CCGG C^CGG 430 CCGG NlaIII 96 CATG CATG^ 300 CATG PleI 112 GACTC GAGTC(4/5) 154 GAGTC PstI 8 CTGCAG CTGCA^G 136 CTGCAG SacI 39 GAGCTC GAGCT^C 244 GAGCTC StyI 44 CCTTGG C^C[AT][AT]GG 281 CCAAGG TspRI 351 CAGTG CA[GC]TG[ACGT][ACGT]^ 402 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AluI AspHI BanII BbvI BmyI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BssKI BstEII BstF5I BstNI BstPI Cac8I Cfr13I DdeI EcoO65I EcoRII Fnu4HI FokI HaeIII HphI ItaI MaeIII MnlI MvaI MwoI NlaIV Sau96I ScrFI SfcI TseI Tsp45I