(Linear) (Six-base) MAPSORT of: KIEE5130.seq Check: 4387 from: 1 to: 450 KIEE5130.orf With 182 enzymes: SgfI * January 29, 2014 11:47 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 379 450 Size: 379 71 ApaLI G'TGCA_C Cuts at: 0 224 450 Size: 224 226 ApoI r'AATT_y Cuts at: 0 314 450 Size: 314 136 AvaI C'yCGr_G Cuts at: 0 18 450 Size: 18 432 BanI G'GyrC_C Cuts at: 0 54 450 Size: 54 396 BanII G_rGCy'C Cuts at: 0 43 164 248 450 Size: 43 121 84 202 Fragments arranged by size: 202 121 84 43 BbsI GAAGACnn'nnnn_ Cuts at: 0 383 450 Size: 383 67 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 230 264 319 353 450 Size: 230 34 55 34 97 Fragments arranged by size: 230 97 55 34 34 BlpI GC'TnA_GC Cuts at: 0 37 450 Size: 37 413 Bme1580I G_kGCm'C Cuts at: 0 228 450 Size: 228 222 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 129 450 Size: 129 321 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 450 Size: 124 326 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 44 450 Size: 44 406 BsaI GGTCTCn'nnnn_ Cuts at: 0 258 450 Size: 258 192 BsaWI w'CCGG_w Cuts at: 0 429 450 Size: 429 21 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 277 307 450 Size: 277 30 143 Fragments arranged by size: 277 143 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 94 97 214 370 450 Size: 94 3 117 156 80 Fragments arranged by size: 156 117 94 80 3 BsiEI CG_ry'CG Cuts at: 0 234 450 Size: 234 216 BsiHKAI G_wGCw'C Cuts at: 0 43 228 248 450 Size: 43 185 20 202 Fragments arranged by size: 202 185 43 20 Bsp1286I G_dGCh'C Cuts at: 0 43 164 228 248 450 Size: 43 121 64 20 202 Fragments arranged by size: 202 121 64 43 20 BsrFI r'CCGG_y Cuts at: 0 251 450 Size: 251 199 BstEII G'GTnAC_C Cuts at: 0 87 204 438 450 Size: 87 117 234 12 Fragments arranged by size: 234 117 87 12 BstXI CCAn_nnnn'nTGG Cuts at: 0 102 178 450 Size: 102 76 272 Fragments arranged by size: 272 102 76 BtsI GCAGTG_nn' Cuts at: 0 357 450 Size: 357 93 DraIII CAC_nnn'GTG Cuts at: 0 223 450 Size: 223 227 EaeI y'GGCC_r Cuts at: 0 231 450 Size: 231 219 EagI C'GGCC_G Cuts at: 0 231 450 Size: 231 219 EcoICRI GAG'CTC Cuts at: 0 41 246 450 Size: 41 205 204 Fragments arranged by size: 205 204 41 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 379 450 Size: 124 255 71 Fragments arranged by size: 255 124 71 HaeII r_GCGC'y Cuts at: 0 258 450 Size: 258 192 HincII GTy'rAC Cuts at: 0 296 450 Size: 296 154 HpaI GTT'AAC Cuts at: 0 296 450 Size: 296 154 MslI CAynn'nnrTG Cuts at: 0 94 450 Size: 94 356 MspA1I CmG'CkG Cuts at: 0 36 364 450 Size: 36 328 86 Fragments arranged by size: 328 86 36 NaeI GCC'GGC Cuts at: 0 253 450 Size: 253 197 NgoMIV G'CCGG_C Cuts at: 0 251 450 Size: 251 199 PfoI T'CCnGG_A Cuts at: 0 101 450 Size: 101 349 PstI C_TGCA'G Cuts at: 0 12 140 450 Size: 12 128 310 Fragments arranged by size: 310 128 12 SacI G_AGCT'C Cuts at: 0 43 248 450 Size: 43 205 202 Fragments arranged by size: 205 202 43 SfcI C'TryA_G Cuts at: 0 8 136 407 450 Size: 8 128 271 43 Fragments arranged by size: 271 128 43 8 SmlI C'TyrA_G Cuts at: 0 59 450 Size: 59 391 StyI C'CwwG_G Cuts at: 0 44 281 450 Size: 44 237 169 Fragments arranged by size: 237 169 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 108 450 Size: 108 342 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 99 450 Size: 99 351 Enzymes that do cut: AcuI ApaLI ApoI AvaI BanI BanII BbsI BcgI BlpI Bme1580I BplI BpmI BpuEI BsaI BsaWI BsaXI BseRI BsiEI BsiHKAI Bsp1286I BsrFI BstEII BstXI BtsI DraIII EaeI EagI EcoICRI Eco57MI HaeII HincII HpaI MslI MspA1I NaeI NgoMIV PfoI PstI SacI SfcI SmlI StyI TaqII XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI Bpu10I BsaAI BsaBI BsaHI BseYI BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EarI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII KasI KpnI MfeI MluI MmeI MscI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI