################################## # NO CUT ENZYMES ( 204 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI AluI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MluI MluNI MroI MscI MseI MslI MspA1I MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI Sau96I SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AlwI 612 GATCC GGATC(4/5) Asp700I 106 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BsmAI 411 GTCTC GTCTC(1/5) BsmFI 468 GGGAC GGGAC(10/14) BsmI 484 GAATGC GAATGC(1/-1) BspMI 150 ACCTGC ACCTGC(4/8) BsrFI 488 GCCGGT [GA]^CCGG[CT] BstYI 611 AGATCC [GA]^GATC[CT] Cac8I 360 GCAGGC GC[ACGT]^[ACGT]GC Cfr10I 488 GCCGGT [GA]^CCGG[CT] DpnII 612 GATC ^GATC EarI 397 GAAGAG CTCTTC(1/4) HapII 489 CCGG C^CGG HpaII 489 CCGG C^CGG HphI 604 GGTGA GGTGA(8/7) Ksp632I 397 GAAGAG CTCTTC(1/4) MboI 612 GATC ^GATC MflI 611 AGATCC [GA]^GATC[CT] MspI 489 CCGG C^CGG NdeII 612 GATC ^GATC NspI 83 GCATGT [GA]CATG^[CT] PleI 235 GACTC GAGTC(4/5) SapI 397 GAAGAGC GCTCTTC(1/4) Sau3AI 612 GATC ^GATC SfcI 296 CTGTAG C^T[GA][CT]AG SmlI 5 CTCAAG C^T[CT][GA]AG Tsp45I 279 GTCAC ^GT[GC]AC XhoII 611 AGATCC [GA]^GATC[CT] XmnI 106 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AlwNI 290 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG 338 CAGTACCTG BsgI 358 GTGCAG GTGCAG(16/14) 453 GTGCAG Bsp1407I 513 TGTACA T^GTACA 531 TGTACA BsrGI 513 TGTACA T^GTACA 531 TGTACA DdeI 525 CTCAG C^T[ACGT]AG 569 CTGAG MaeIII 279 GTCAC ^GT[ACGT]AC 521 GTTAC MfeI 310 CAATTG C^AATTG 537 CAATTG MnlI 70 CCTC CCTC(7/6) 158 CCTC MunI 310 CAATTG C^AATTG 537 CAATTG NlaIII 84 CATG CATG^ 450 CATG SfaNI 562 GATGC GCATC(5/9) 301 GCATC SspBI 513 TGTACA T^GTACA 531 TGTACA TspRI 45 CACTG CA[GC]TG[ACGT][ACGT]^ 207 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AfaI BbvI BsrI Fnu4HI HinfI ItaI MboII MwoI RsaI TfiI TseI Tsp509I