################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BlnI BmyI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AciI 686 GCGG CCGC(-3/-1) AclI 709 AACGTT AA^CGTT AlwI 822 GGATC GGATC(4/5) Asp700I 851 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 362 TGGCCA TGG^CCA BbvI 235 GCTGC GCAGC(8/12) BclI 813 TGATCA T^GATCA BcnI 597 CCCGG CC^[GC]GG BglI 664 GCCAATTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 644 AGATCT A^GATCT BlpI 681 GCTGAGC GC^T[ACGT]AGC BpmI 178 CTGGAG CTGGAG(16/14) Bpu1102I 681 GCTGAGC GC^T[ACGT]AGC BsmAI 97 GAGAC GTCTC(1/5) BsmI 715 GCATTC GAATGC(1/-1) Bsp1407I 654 TGTACA T^GTACA BsrBI 684 GAGCGG CCGCTC(-3/-3) BsrDI 231 CATTGC GCAATG(2/0) BsrGI 654 TGTACA T^GTACA BssSI 435 CACGAG CACGAG(-5/-1) BstYI 644 AGATCT [GA]^GATC[CT] CelII 681 GCTGAGC GC^T[ACGT]AGC DraII 312 AGGACCT [GA]G^G[ACGT]CC[CT] EarI 22 CTCTTC CTCTTC(1/4) EcoNI 126 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 312 AGGACCT [GA]G^G[ACGT]CC[CT] FbaI 813 TGATCA T^GATCA HapII 598 CCGG C^CGG HpaII 598 CCGG C^CGG Ksp632I 22 CTCTTC CTCTTC(1/4) MaeII 710 ACGT A^CGT MboII 23 TCTTC GAAGA(8/7) MflI 644 AGATCT [GA]^GATC[CT] MluNI 362 TGGCCA TGG^CCA MscI 362 TGGCCA TGG^CCA MspA1I 190 CAGCTG C[AC]G^C[GT]G MspI 598 CCGG C^CGG NciI 597 CCCGG CC^[GC]GG NspI 660 ACATGC [GA]CATG^[CT] PflMI 354 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 312 AGGACCT [GA]G^G[AT]CC[CT] PshAI 99 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 709 AACGTT AA^CGTT PvuII 190 CAGCTG CAG^CTG SexAI 125 ACCTGGT A^CC[AT]GGT SfcI 166 CTACAG C^T[GA][CT]AG SspBI 654 TGTACA T^GTACA TseI 235 GCTGC G^C[AT]GC Van91I 354 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 644 AGATCT [GA]^GATC[CT] XmnI 851 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 445 AAATTC [GA]^AATT[CT] 890 AAATTC AfaI 12 GTAC GT^AC 655 GTAC AlwNI 311 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 355 CAGTATCTG ApoI 445 AAATTC [GA]^AATT[CT] 890 AAATTC AvaII 162 GGACC G^G[AT]CC 313 GGACC BfaI 118 CTAG C^TAG 487 CTAG Cfr13I 162 GGACC G^G[ACGT]CC 313 GGACC EaeI 362 TGGCCA [CT]^GGCC[GA] 687 CGGCCA Fnu4HI 235 GCTGC GC^[ACGT]GC 686 GCGGC HphI 129 GGTGA GGTGA(8/7) 816 TCACC ItaI 235 GCTGC GC^[ACGT]GC 686 GCGGC MaeI 118 CTAG C^TAG 487 CTAG MaeIII 106 GTCAC ^GT[ACGT]AC 616 GTCAC MslI 43 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 895 CACATCTATG NlaIV 112 GGCTCC GG[ACGT]^[ACGT]CC 496 GGAGCC PleI 601 GACTC GAGTC(4/5) 174 GAGTC RsaI 12 GTAC GT^AC 655 GTAC Sau96I 162 GGACC G^G[ACGT]CC 313 GGACC SspI 422 AATATT AAT^ATT 517 AATATT TfiI 349 GATTC G^A[AT]TC 509 GAATC Tsp45I 106 GTCAC ^GT[GC]AC 616 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AluI BsaJI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII FokI HaeIII HinfI MboI MnlI MvaI MwoI NdeII NlaIII Sau3AI ScrFI SfaNI Tsp509I TspRI