Restriction map for
pFN21AB7612
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AocI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AviII BalI BamHI
BbvCI BciVI BfrI BglII BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DraIII EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MamI MfeI
MluI MluNI MroI MscI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I XbaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AhdI 511 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 99 GATCC GGATC(4/5)
Aor51HI 725 AGCGCT AGC^GCT
AspEI 511 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 238 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 987 CCTAGG C^CTAGG
BanII 690 GAGCCC G[GA]GC[CT]^C
BbeI 487 GGCGCC GGCGC^C
BclI 45 TGATCA T^GATCA
BfaI 988 CTAG C^TAG
BglI 275 GCCAGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 987 CCTAGG C^CTAGG
BlpI 909 GCTCAGC GC^T[ACGT]AGC
BpmI 925 CTCCAG CTGGAG(16/14)
Bpu1102I 909 GCTCAGC GC^T[ACGT]AGC
BsaI 963 GGTCTC GGTCTC(1/5)
BseRI 996 CTCCTC GAGGAG(10/8)
BsiEI 452 CGGCCG CG[GA][CT]^CG
BspMI 622 ACCTGC ACCTGC(4/8)
BssHII 444 GCGCGC G^CGCGC
BssSI 403 CACGAG CACGAG(-5/-1)
BstYI 98 AGATCC [GA]^GATC[CT]
CelII 909 GCTCAGC GC^T[ACGT]AGC
DrdI 1014 GACCTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 452 CGGCCG C^GGCCG
Eam1105I 511 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 452 CGGCCG C^GGCCG
Eco47III 725 AGCGCT AGC^GCT
Eco52I 452 CGGCCG C^GGCCG
EcoT14I 987 CCTAGG C^C[AT][AT]GG
FbaI 45 TGATCA T^GATCA
KasI 487 GGCGCC G^GCGCC
MaeI 988 CTAG C^TAG
MaeIII 1012 GTGAC ^GT[ACGT]AC
MflI 98 AGATCC [GA]^GATC[CT]
NarI 487 GGCGCC GG^CGCC
PflFI 238 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 766 CTGCAG CTGCA^G
SexAI 299 ACCTGGT A^CC[AT]GGT
SfcI 766 CTGCAG C^T[GA][CT]AG
SfoI 487 GGCGCC GGC^GCC
SmaI 64 CCCGGG CCC^GGG
StyI 987 CCTAGG C^C[AT][AT]GG
Tsp45I 1012 GTGAC ^GT[GC]AC
TspRI 872 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 238 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 98 AGATCC [GA]^GATC[CT]
XmaCI 64 CCCGGG C^CCGGG
XmaI 64 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 487 GGCGCC G[GA]^CG[CT]C
816 GACGCC
Alw44I 195 GTGCAC G^TGCAC
550 GTGCAC
ApaLI 195 GTGCAC G^TGCAC
550 GTGCAC
AvaII 620 GGACC G^G[AT]CC
939 GGACC
BanI 487 GGCGCC G^G[CT][GA]CC
1056 GGTGCC
BbrPI 198 CACGTG CAC^GTG
547 CACGTG
BbsI 219 GAAGAC GAAGAC(2/6)
567 GAAGAC
BcgI 572 CGACCCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
197 GCACGTGCTTCG
BpuAI 219 GAAGAC GAAGAC(2/6)
567 GAAGAC
BsaAI 198 CACGTG [CT]AC^GT[GA]
547 CACGTG
BsaHI 487 GGCGCC G[GA]^CG[CT]C
816 GACGCC
BspHI 656 TCATGA T^CATGA
755 TCATGA
BstF5I 702 CATCC GGATG(2/0)
840 CATCC
BstXI 74 CCATGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1046 CCAACAGTATGG
DdeI 688 CTGAG C^T[ACGT]AG
910 CTCAG
DpnII 46 GATC ^GATC
99 GATC
EaeI 71 CGGCCA [CT]^GGCC[GA]
452 CGGCCG
FokI 702 CATCC GGATG(9/13)
840 CATCC
Hin1I 487 GGCGCC G[GA]^CG[CT]C
816 GACGCC
HphI 43 GGTGA GGTGA(8/7)
303 GGTGA
MboI 46 GATC ^GATC
99 GATC
MslI 75 CATGTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
750 CATTCTCATG
NaeI 454 GCCGGC GCC^GGC
524 GCCGGC
NdeII 46 GATC ^GATC
99 GATC
NgoMIV 454 GCCGGC G^CCGGC
524 GCCGGC
PaeR7I 136 CTCGAG C^TCGAG
439 CTCGAG
PflMI 177 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
954 CCAACAGCTGG
PmaCI 198 CACGTG CAC^GTG
547 CACGTG
PmlI 198 CACGTG CAC^GTG
547 CACGTG
RcaI 656 TCATGA T^CATGA
755 TCATGA
Sau3AI 46 GATC ^GATC
99 GATC
SfaNI 257 GCATC GCATC(5/9)
501 GCATC
Van91I 177 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
954 CCAACAGCTGG
XcmI 269 CCAAGTGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
366 CCAGCTGCTGCCTGG
XhoI 136 CTCGAG C^TCGAG
439 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI BbvI BcnI BmyI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstAPI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DsaI EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI KspI MaeII
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PleI PvuII RsaI SacII Sau96I
ScrFI SmlI TaqI TseI TthHB8I