(Circular) (Six-base) MAPSORT of: pFN21AB7612.seq Check: 3328 from: 1 to: 5782 pFN21AB7612 5782 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3125 1937 Size: 1188 4594 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2275 2789 3845 5229 5497 1632 Size: 643 514 1056 1384 268 1917 Fragments arranged by size: 1917 1384 1056 643 514 268 AfeI AGC'GCT Cuts at: 2732 2732 Size: 5782 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 4681 5658 1203 Size: 3478 977 1327 Fragments arranged by size: 3478 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 2521 4511 2521 Size: 1990 3792 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5782 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AlwNI CAG_nnn'CTG Cuts at: 2188 2377 2812 3525 5097 2188 Size: 189 435 713 1572 2873 Fragments arranged by size: 2873 1572 713 435 189 ApaLI G'TGCA_C Cuts at: 2200 2555 3839 4995 2200 Size: 355 1284 1156 2987 Fragments arranged by size: 2987 1284 1156 355 ApoI r'AATT_y Cuts at: 1493 3091 3317 3469 5578 1493 Size: 1598 226 152 2109 1697 Fragments arranged by size: 2109 1697 1598 226 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2069 2141 2444 3107 1870 Size: 73 126 72 303 663 4545 Fragments arranged by size: 4545 663 303 126 73 72 AvrII C'CTAG_G Cuts at: 2992 2992 Size: 5782 BamHI G'GATC_C Cuts at: 3112 3479 3112 Size: 367 5415 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2492 3061 3103 4558 618 Size: 325 217 627 705 569 42 1455 1842 Fragments arranged by size: 1842 1455 705 627 569 325 217 42 BanII G_rGCy'C Cuts at: 1391 2699 3101 1391 Size: 1308 402 4072 Fragments arranged by size: 4072 1308 402 BbeI G_GCGC'C Cuts at: 2496 2496 Size: 5782 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2231 2579 928 Size: 971 332 348 4131 Fragments arranged by size: 4131 971 348 332 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2191 2225 2566 2600 3973 4007 1903 Size: 34 254 34 341 34 1373 34 3678 Fragments arranged by size: 3678 1373 341 254 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 2050 1548 Size: 502 5280 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2286 4393 136 Size: 107 122 71 631 605 614 2107 1525 Fragments arranged by size: 2107 1525 631 614 605 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 2915 3199 2915 Size: 284 5498 Bme1580I G_kGCm'C Cuts at: 1790 2204 2559 3843 4999 1790 Size: 414 355 1284 1156 2573 Fragments arranged by size: 2573 1284 1156 414 355 BmgBI CAC'GTC Cuts at: 1318 2199 3016 1318 Size: 881 817 4084 Fragments arranged by size: 4084 881 817 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2826 3513 4471 Size: 416 53 83 399 24 1409 687 958 Cuts at: 4471 442 Size: 1753 Fragments arranged by size: 1753 1409 958 687 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3004 3004 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2915 4442 1239 Size: 1676 1527 2579 Fragments arranged by size: 2579 1676 1527 Bpu10I CC'TnA_GC Cuts at: 2693 2693 Size: 5782 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2147 3907 4772 5070 2147 Size: 1760 865 298 2859 Fragments arranged by size: 2859 1760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2974 4445 882 Size: 617 1475 1471 2219 Fragments arranged by size: 2219 1475 1471 617 BsaAI yAC'GTr Cuts at: 493 1204 2205 2554 3573 493 Size: 711 1001 349 1019 2702 Fragments arranged by size: 2702 1019 1001 711 349 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2493 2822 3589 3971 Size: 53 83 186 936 960 329 767 382 Cuts at: 3971 4606 275 Size: 635 1451 Fragments arranged by size: 1451 960 936 767 635 382 329 186 83 53 BsaWI w'CCGG_w Cuts at: 4215 4613 4887 5034 5445 4215 Size: 398 274 147 411 4552 Fragments arranged by size: 4552 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3005 3035 1178 Size: 30 165 30 1602 30 3925 Fragments arranged by size: 3925 1602 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2992 1186 Size: 1806 3976 BseYI C'CCAG_C Cuts at: 2519 4985 5481 2519 Size: 2466 496 2820 Fragments arranged by size: 2820 2466 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2197 2383 2597 2885 2906 1163 Size: 573 461 186 214 288 21 4039 Fragments arranged by size: 4039 573 461 288 214 186 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2460 3232 3993 5021 1540 Size: 275 186 459 772 761 1028 2301 Fragments arranged by size: 2301 1028 772 761 459 275 186 BsiHKAI G_wGCw'C Cuts at: 2204 2409 2559 3843 3928 4999 2204 Size: 205 150 1284 85 1071 2987 Fragments arranged by size: 2987 1284 1071 205 150 85 BsmI GAATG_Cn' Cuts at: 3293 3386 3293 Size: 93 5689 BsmBI CGTCTCn'nnnn_ Cuts at: 2189 2211 3071 2189 Size: 22 860 4900 Fragments arranged by size: 4900 860 22 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2204 2409 2559 2699 3101 3843 3928 Size: 399 414 205 150 140 402 742 85 Cuts at: 3928 4999 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 414 402 399 205 150 140 85 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 2661 2760 3671 5401 2661 Size: 99 911 1730 3042 Fragments arranged by size: 3042 1730 911 99 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2636 3137 844 Size: 1792 501 3489 Fragments arranged by size: 3489 1792 501 BsrBI CCG'CTC Cuts at: 1394 1823 3669 1394 Size: 429 1846 3507 Fragments arranged by size: 3507 1846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2459 2529 2687 4426 4613 1700 Size: 110 649 70 158 1739 187 2869 Fragments arranged by size: 2869 1739 649 187 158 110 70 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 2449 1925 Size: 524 5258 BssSI C'ACGA_G Cuts at: 2408 3842 4854 2408 Size: 1434 1012 3336 Fragments arranged by size: 3336 1434 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2268 2413 2620 794 Size: 1474 145 207 3956 Fragments arranged by size: 3956 1474 207 145 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstXI CCAn_nnnn'nTGG Cuts at: 2086 3058 2086 Size: 972 4810 BstYI r'GATC_y Cuts at: 1972 2103 3112 3479 3864 3881 5333 5419 5431 Size: 131 1009 367 385 17 1452 86 12 Cuts at: 5431 5777 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1009 385 367 346 131 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2431 2454 2497 2645 2699 2903 Size: 552 939 427 23 43 148 54 204 Cuts at: 2903 3072 513 Size: 169 3223 Fragments arranged by size: 3223 939 552 427 204 169 148 54 43 23 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5043 Fragments arranged by size: 5043 683 56 BtsI GCAGTG_nn' Cuts at: 2883 3298 4092 4112 2883 Size: 415 794 20 4553 Fragments arranged by size: 4553 794 415 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3025 4789 809 Size: 507 1709 1764 1802 Fragments arranged by size: 1802 1764 1709 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2457 3229 Size: 54 1425 50 275 18 246 381 772 Cuts at: 3229 4118 5747 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 772 381 275 246 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2457 3229 1537 Size: 275 645 772 4090 Fragments arranged by size: 4090 772 645 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3712 1353 Size: 2359 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2837 4369 4743 4889 387 Size: 1064 138 1248 1532 374 146 1280 Fragments arranged by size: 1532 1280 1248 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2275 2789 2915 3845 4442 5229 5497 Size: 393 643 514 126 930 597 787 268 Cuts at: 5497 1239 Size: 1524 Fragments arranged by size: 1524 930 787 643 597 514 393 268 126 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 2496 2734 2915 4929 1678 Size: 818 238 181 2014 2531 Fragments arranged by size: 2531 2014 818 238 181 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2378 2410 2507 2539 3005 Size: 32 135 32 806 32 97 32 466 Cuts at: 3005 3037 4423 4455 4497 4529 1373 Size: 32 1386 32 42 32 2626 Fragments arranged by size: 2626 1386 806 466 135 97 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3126 3372 669 Size: 988 281 1188 246 3079 Fragments arranged by size: 3079 1188 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5782 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KasI G'GCGC_C Cuts at: 2492 2492 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2983 4896 5080 1305 Size: 1678 1913 184 2007 Fragments arranged by size: 2007 1913 1678 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3960 Fragments arranged by size: 3960 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2084 2759 3740 4099 4258 518 Size: 648 918 675 981 359 159 2042 Fragments arranged by size: 2042 981 918 675 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2352 2374 2456 2499 2611 2647 2674 2701 Size: 746 22 82 43 112 36 27 27 Cuts at: 2701 2770 2965 3198 3875 5023 5268 1606 Size: 69 195 233 677 1148 245 2120 Fragments arranged by size: 2120 1148 746 677 245 233 195 112 82 69 43 36 27 27 22 NaeI GCC'GGC Cuts at: 2461 2531 2461 Size: 70 5712 NarI GG'CG_CC Cuts at: 2493 2493 Size: 5782 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4291 Fragments arranged by size: 4291 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NgoMIV G'CCGG_C Cuts at: 2459 2529 2459 Size: 70 5712 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 3140 4685 1132 Size: 97 1911 1545 2229 Fragments arranged by size: 2229 1911 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5782 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 2188 2965 1108 Size: 1080 777 3925 Fragments arranged by size: 3925 1080 777 PfoI T'CCnGG_A Cuts at: 3039 3039 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PmlI CAC'GTG Cuts at: 2205 2554 2205 Size: 349 5433 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PspXI vC'TCGA_Gb Cuts at: 2141 2444 2141 Size: 303 5479 PstI C_TGCA'G Cuts at: 830 2775 3134 830 Size: 1945 359 3478 Fragments arranged by size: 3478 1945 359 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2374 2611 2674 2770 2965 2374 Size: 237 63 96 195 5191 Fragments arranged by size: 5191 237 195 96 63 SacII CC_GC'GG Cuts at: 2457 2500 2648 2702 2457 Size: 43 148 54 5537 Fragments arranged by size: 5537 148 54 43 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SexAI A'CCwGG_T Cuts at: 2304 2304 Size: 5782 SfcI C'TryA_G Cuts at: 826 1045 2771 3130 4265 4946 5137 826 Size: 219 1726 359 1135 681 191 1471 Fragments arranged by size: 1726 1471 1135 681 359 219 191 SfoI GGC'GCC Cuts at: 2494 2494 Size: 5782 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 2071 3109 1872 Size: 199 1038 4545 Fragments arranged by size: 4545 1038 199 SmlI C'TyrA_G Cuts at: 820 1017 1943 2141 2162 2444 3886 4787 5049 Size: 197 926 198 21 282 1442 901 262 Cuts at: 5049 820 Size: 1553 Fragments arranged by size: 1553 1442 926 901 282 262 198 197 21 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5782 StyI C'CwwG_G Cuts at: 513 1065 2004 2992 3221 513 Size: 552 939 988 229 3074 Fragments arranged by size: 3074 988 939 552 229 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2027 2036 3809 3826 3979 4164 4609 Size: 1255 447 9 1773 17 153 185 445 Cuts at: 4609 325 Size: 1498 Fragments arranged by size: 1773 1498 1255 447 445 185 153 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4028 5740 96 Size: 274 80 33 51 1443 2051 1712 138 Fragments arranged by size: 2051 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2183 2215 5742 5774 2183 Size: 32 3527 32 2191 Fragments arranged by size: 3527 2191 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2246 1316 Size: 930 4852 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2281 2378 2281 Size: 97 5685 XhoI C'TCGA_G Cuts at: 1943 2141 2444 1943 Size: 198 303 5281 Fragments arranged by size: 5281 303 198 XmaI C'CCGG_G Cuts at: 1870 2069 3107 1870 Size: 199 1038 4545 Fragments arranged by size: 4545 1038 199 XmnI GAAnn'nnTTC Cuts at: 3911 3911 Size: 5782 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspOMI PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI