Restriction map for
pFN21AB7612
(Linear) (Six-base) MAPSORT of: KIBB7612.seq Check: 2370 from: 1 to: 1077
KIBB7612.orf
With 182 enzymes: SgfI *
January 29, 2014 11:43 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 784 1077
Size: 270 514 293
Fragments arranged by size:
514 293 270
AfeI AGC'GCT
Cuts at: 0 727 1077
Size: 727 350
AhdI GACnn_n'nnGTC
Cuts at: 0 516 1077
Size: 516 561
AlwNI CAG_nnn'CTG
Cuts at: 0 183 372 807 1077
Size: 183 189 435 270
Fragments arranged by size:
435 270 189 183
ApaLI G'TGCA_C
Cuts at: 0 195 550 1077
Size: 195 355 527
Fragments arranged by size:
527 355 195
AvaI C'yCGr_G
Cuts at: 0 64 136 439 1077
Size: 64 72 303 638
Fragments arranged by size:
638 303 72 64
AvrII C'CTAG_G
Cuts at: 0 987 1077
Size: 987 90
BanI G'GyrC_C
Cuts at: 0 487 1056 1077
Size: 487 569 21
Fragments arranged by size:
569 487 21
BanII G_rGCy'C
Cuts at: 0 694 1077
Size: 694 383
BbeI G_GCGC'C
Cuts at: 0 491 1077
Size: 491 586
BbsI GAAGACnn'nnnn_
Cuts at: 0 226 574 1077
Size: 226 348 503
Fragments arranged by size:
503 348 226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 186 220 561 595 1077
Size: 186 34 341 34 482
Fragments arranged by size:
482 341 186 34 34
BclI T'GATC_A
Cuts at: 0 45 1077
Size: 45 1032
BglI GCCn_nnn'nGGC
Cuts at: 0 281 1077
Size: 281 796
BlpI GC'TnA_GC
Cuts at: 0 910 1077
Size: 910 167
Bme1580I G_kGCm'C
Cuts at: 0 199 554 1077
Size: 199 355 523
Fragments arranged by size:
523 355 199
BmgBI CAC'GTC
Cuts at: 0 194 1011 1077
Size: 194 817 66
Fragments arranged by size:
817 194 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 821 1077
Size: 821 256
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 999 1077
Size: 999 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1077
Size: 910 167
Bpu10I CC'TnA_GC
Cuts at: 0 688 1077
Size: 688 389
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1077
Size: 142 935
BsaI GGTCTCn'nnnn_
Cuts at: 0 969 1077
Size: 969 108
BsaAI yAC'GTr
Cuts at: 0 200 549 1077
Size: 200 349 528
Fragments arranged by size:
528 349 200
BsaHI Gr'CG_yC
Cuts at: 0 488 817 1077
Size: 488 329 260
Fragments arranged by size:
488 329 260
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1000 1030 1077
Size: 1000 30 47
Fragments arranged by size:
1000 47 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 987 1077
Size: 987 90
BseYI C'CCAG_C
Cuts at: 0 514 1077
Size: 514 563
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 378 592 880 901 1077
Size: 192 186 214 288 21 176
Fragments arranged by size:
288 214 192 186 176 21
BsiEI CG_ry'CG
Cuts at: 0 455 1077
Size: 455 622
BsiHKAI G_wGCw'C
Cuts at: 0 199 404 554 1077
Size: 199 205 150 523
Fragments arranged by size:
523 205 199 150
BsmBI CGTCTCn'nnnn_
Cuts at: 0 184 206 1066 1077
Size: 184 22 860 11
Fragments arranged by size:
860 184 22 11
Bsp1286I G_dGCh'C
Cuts at: 0 199 404 554 694 1077
Size: 199 205 150 140 383
Fragments arranged by size:
383 205 199 150 140
BspHI T'CATG_A
Cuts at: 0 656 755 1077
Size: 656 99 322
Fragments arranged by size:
656 322 99
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 631 1077
Size: 631 446
BsrFI r'CCGG_y
Cuts at: 0 454 524 682 1077
Size: 454 70 158 395
Fragments arranged by size:
454 395 158 70
BssHII G'CGCG_C
Cuts at: 0 444 1077
Size: 444 633
BssSI C'ACGA_G
Cuts at: 0 403 1077
Size: 403 674
BstAPI GCAn_nnn'nTGC
Cuts at: 0 263 408 615 1077
Size: 263 145 207 462
Fragments arranged by size:
462 263 207 145
BstXI CCAn_nnnn'nTGG
Cuts at: 0 81 1053 1077
Size: 81 972 24
Fragments arranged by size:
972 81 24
BstYI r'GATC_y
Cuts at: 0 98 1077
Size: 98 979
BtgI C'CryG_G
Cuts at: 0 426 449 492 640 694 898 1067 1077
Size: 426 23 43 148 54 204 169 10
Fragments arranged by size:
426 204 169 148 54 43 23 10
BtsI GCAGTG_nn'
Cuts at: 0 878 1077
Size: 878 199
DrdI GACnn_nn'nnGTC
Cuts at: 0 1020 1077
Size: 1020 57
EaeI y'GGCC_r
Cuts at: 0 71 452 1077
Size: 71 381 625
Fragments arranged by size:
625 381 71
EagI C'GGCC_G
Cuts at: 0 452 1077
Size: 452 625
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 832 1077
Size: 832 245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 784 910 1077
Size: 270 514 126 167
Fragments arranged by size:
514 270 167 126
HaeII r_GCGC'y
Cuts at: 0 491 729 910 1077
Size: 491 238 181 167
Fragments arranged by size:
491 238 181 167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 502 534 1000 1032 1077
Size: 373 32 97 32 466 32 45
Fragments arranged by size:
466 373 97 45 32 32 32
KasI G'GCGC_C
Cuts at: 0 487 1077
Size: 487 590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 978 1077
Size: 978 99
MslI CAynn'nnrTG
Cuts at: 0 79 754 1077
Size: 79 675 323
Fragments arranged by size:
675 323 79
MspA1I CmG'CkG
Cuts at: 0 347 369 451 494 606 642 669 696
Size: 347 22 82 43 112 36 27 27
Cuts at: 696 765 960 1077
Size: 69 195 117
Fragments arranged by size:
347 195 117 112 82 69 43 36
27 27 22
NaeI GCC'GGC
Cuts at: 0 456 526 1077
Size: 456 70 551
Fragments arranged by size:
551 456 70
NarI GG'CG_CC
Cuts at: 0 488 1077
Size: 488 589
NgoMIV G'CCGG_C
Cuts at: 0 454 524 1077
Size: 454 70 553
Fragments arranged by size:
553 454 70
PflMI CCAn_nnn'nTGG
Cuts at: 0 183 960 1077
Size: 183 777 117
Fragments arranged by size:
777 183 117
PfoI T'CCnGG_A
Cuts at: 0 1034 1077
Size: 1034 43
PmlI CAC'GTG
Cuts at: 0 200 549 1077
Size: 200 349 528
Fragments arranged by size:
528 349 200
PspXI vC'TCGA_Gb
Cuts at: 0 136 439 1077
Size: 136 303 638
Fragments arranged by size:
638 303 136
PstI C_TGCA'G
Cuts at: 0 770 1077
Size: 770 307
PvuII CAG'CTG
Cuts at: 0 369 606 669 765 960 1077
Size: 369 237 63 96 195 117
Fragments arranged by size:
369 237 195 117 96 63
SacII CC_GC'GG
Cuts at: 0 452 495 643 697 1077
Size: 452 43 148 54 380
Fragments arranged by size:
452 380 148 54 43
SexAI A'CCwGG_T
Cuts at: 0 299 1077
Size: 299 778
SfcI C'TryA_G
Cuts at: 0 766 1077
Size: 766 311
SfoI GGC'GCC
Cuts at: 0 489 1077
Size: 489 588
SmaI CCC'GGG
Cuts at: 0 66 1077
Size: 66 1011
SmlI C'TyrA_G
Cuts at: 0 136 157 439 1077
Size: 136 21 282 638
Fragments arranged by size:
638 282 136 21
StyI C'CwwG_G
Cuts at: 0 987 1077
Size: 987 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 22 31 1077
Size: 22 9 1046
Fragments arranged by size:
1046 22 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 178 210 1077
Size: 178 32 867
Fragments arranged by size:
867 178 32
Tth111I GACn'n_nGTC
Cuts at: 0 241 1077
Size: 241 836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 276 373 1077
Size: 276 97 704
Fragments arranged by size:
704 276 97
XhoI C'TCGA_G
Cuts at: 0 136 439 1077
Size: 136 303 638
Fragments arranged by size:
638 303 136
XmaI C'CCGG_G
Cuts at: 0 64 1077
Size: 64 1013
Enzymes that do cut:
AcuI AfeI AhdI AlwNI ApaLI AvaI AvrII BanI
BanII BbeI BbsI BcgI BclI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrFI BssHII BssSI BstAPI BstXI
BstYI BtgI BtsI DrdI EaeI EagI EciI Eco57MI
HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI
NgoMIV PflMI PfoI PmlI PspXI PstI PvuII SacII
SexAI SfcI SfoI SmaI SmlI StyI TaqII TstI
Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
BaeI BamHI BbvCI BciVI BfrBI BglII BmtI BsaBI
BsaWI BsiWI BsmI BspEI BsrBI BsrDI BsrGI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EarI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MscI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI XbaI XmnI ZraI