Restriction map for
pFN21AE3171
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
Acc65I AccIII AclI AfaI AflII
AflIII AgeI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AvrII BanI BanII
BbeI BbrPI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraII DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRI EcoRV FbaI
HgaI HincII HindII HindIII HpaI
KasI KpnI MfeI MluI MroI
MunI NarI NciI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 883 GACGTC GACGT^C
AccI 43 GTAGAC GT^[AC][GT]AC
AcsI 591 GAATTT [GA]^AATT[CT]
AcyI 883 GACGTC G[GA]^CG[CT]C
AhdI 766 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 493 CCTCAGG CC^T[ACGT]AGG
ApoI 591 GAATTT [GA]^AATT[CT]
Asp700I 408 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 766 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 816 CTCGGG C^[CT]CG[GA]G
AviII 167 TGCGCA TGC^GCA
BalI 354 TGGCCA TGG^CCA
BamHI 582 GGATCC G^GATCC
BbsI 897 GAAGAC GAAGAC(2/6)
BbvCI 904 GCTGAGG CCTCAGC(-5/-2)
BpuAI 897 GAAGAC GAAGAC(2/6)
BsaBI 1147 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 883 GACGTC G[GA]^CG[CT]C
BsoBI 816 CTCGGG C^[CT]CG[GA]G
BsrDI 244 CATTGC GCAATG(2/0)
BstF5I 1090 GGATG GGATG(2/0)
BstXI 429 CCAAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 493 CCTCAGG CC^T[ACGT]AGG
Eam1105I 766 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 343 GAAGAG CTCTTC(1/4)
Eco81I 493 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1028 ATGCAT ATGCA^T
FokI 1090 GGATG GGATG(9/13)
FseI 225 GGCCGGCC GGCCGG^CC
FspI 167 TGCGCA TGC^GCA
HaeII 920 AGCGCC [GA]GCGC^[CT]
Hin1I 883 GACGTC G[GA]^CG[CT]C
HphI 101 TCACC GGTGA(8/7)
Ksp632I 343 GAAGAG CTCTTC(1/4)
MaeII 884 ACGT A^CGT
MamI 1147 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 354 TGGCCA TGG^CCA
MscI 354 TGGCCA TGG^CCA
MslI 57 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 226 GCCGGC GCC^GGC
NgoMIV 226 GCCGGC G^CCGGC
NsiI 1028 ATGCAT ATGCA^T
NspI 973 GCATGC [GA]CATG^[CT]
PleI 465 GACTC GAGTC(4/5)
PstI 706 CTGCAG CTGCA^G
SfcI 706 CTGCAG C^T[GA][CT]AG
SpeI 379 ACTAGT A^CTAGT
SphI 973 GCATGC GCATG^C
StuI 213 AGGCCT AGG^CCT
TaqI 489 TCGA T^CGA
TthHB8I 489 TCGA T^CGA
XmnI 408 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AvaII 182 GGACC G^G[AT]CC
657 GGTCC
BfaI 380 CTAG C^TAG
1049 CTAG
BpmI 235 CTGGAG CTGGAG(16/14)
752 CTGGAG
BsrFI 6 GCCGGT [GA]^CCGG[CT]
226 GCCGGC
BsrI 1108 ACTGG ACTGG(1/-1)
695 CCAGT
BstYI 203 GGATCT [GA]^GATC[CT]
582 GGATCC
CfoI 168 GCGC GCG^C
921 GCGC
Cfr10I 6 GCCGGT [GA]^CCGG[CT]
226 GCCGGC
Cfr13I 182 GGACC G^G[ACGT]CC
657 GGTCC
DdeI 494 CTCAG C^T[ACGT]AG
905 CTGAG
DsaI 30 CCGCGG C^C[GA][CT]GG
49 CCGCGG
HapII 7 CCGG C^CGG
227 CCGG
HhaI 168 GCGC GCG^C
921 GCGC
HinP1I 168 GCGC G^CGC
921 GCGC
HpaII 7 CCGG C^CGG
227 CCGG
KspI 30 CCGCGG CCGC^GG
49 CCGCGG
MaeI 380 CTAG C^TAG
1049 CTAG
MboII 343 GAAGA GAAGA(8/7)
897 GAAGA
MflI 203 GGATCT [GA]^GATC[CT]
582 GGATCC
MseI 578 TTAA T^TAA
595 TTAA
MspA1I 30 CCGCGG C[AC]G^C[GT]G
49 CCGCGG
MspI 7 CCGG C^CGG
227 CCGG
SacII 30 CCGCGG CCGC^GG
49 CCGCGG
Sau96I 182 GGACC G^G[ACGT]CC
657 GGTCC
SmlI 274 CTTGAG C^T[CT][GA]AG
403 CTTGAG
Tru9I 578 TTAA T^TAA
595 TTAA
XhoII 203 GGATCT [GA]^GATC[CT]
582 GGATCC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
BbvI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstNI
BstUI Cac8I DpnII EaeI EcoNI
EcoRII EcoT14I FauI Fnu4HI HaeIII
HinfI ItaI MaeIII MboI MnlI
MvaI MvnI MwoI NdeII NlaIII
NlaIV Sau3AI ScrFI SfaNI StyI
TfiI TseI Tsp45I Tsp509I TspRI