(Circular) (Six-base) MAPSORT of: pFN21AE3171.seq Check: 904 from: 1 to: 5914 pFN21AE3171 5914 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2892 3724 278 Size: 53 83 186 936 1356 832 2468 Fragments arranged by size: 2468 1356 936 832 186 83 53 AccI GT'mk_AC Cuts at: 1937 2049 3257 1937 Size: 112 1208 4594 Fragments arranged by size: 4594 1208 112 Acc65I G'GTAC_C Cuts at: 3235 3235 Size: 5914 AclI AA'CG_TT Cuts at: 1563 4041 4414 1563 Size: 2478 373 3063 Fragments arranged by size: 3063 2478 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2646 3977 5361 5629 1632 Size: 1014 1331 1384 268 1917 Fragments arranged by size: 1917 1384 1331 1014 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5717 AflIII A'CryG_T Cuts at: 1203 4813 5790 1203 Size: 3610 977 1327 Fragments arranged by size: 3610 1327 977 AgeI A'CCGG_T Cuts at: 4745 4745 Size: 5914 AhdI GACnn_n'nnGTC Cuts at: 2776 4643 2776 Size: 1867 4047 AleI CACnn'nnGTG Cuts at: 1166 2066 1166 Size: 900 5014 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2198 1184 Size: 1014 4900 AlwNI CAG_nnn'CTG Cuts at: 3657 5229 3657 Size: 1572 4342 ApaLI G'TGCA_C Cuts at: 3971 5127 3971 Size: 1156 4758 ApoI r'AATT_y Cuts at: 1493 2596 3223 3449 3601 5710 1493 Size: 1103 627 226 152 2109 1697 Fragments arranged by size: 2109 1697 1103 627 226 152 AseI AT'TA_AT Cuts at: 160 1032 4468 160 Size: 872 3436 1606 Fragments arranged by size: 3436 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 AvaI C'yCGr_G Cuts at: 1870 1943 2821 3239 1870 Size: 73 878 418 4545 Fragments arranged by size: 4545 878 418 73 BamHI G'GATC_C Cuts at: 2587 3244 3611 2587 Size: 657 367 4890 Fragments arranged by size: 4890 657 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3235 4690 618 Size: 325 217 627 1448 1455 1842 Fragments arranged by size: 1842 1455 1448 627 325 217 BanII G_rGCy'C Cuts at: 1391 3233 1391 Size: 1842 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2909 928 Size: 971 1010 3933 Fragments arranged by size: 3933 1010 971 BbvCI CC'TCA_GC Cuts at: 2910 2910 Size: 5914 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4105 4139 1903 Size: 34 2168 34 3678 Fragments arranged by size: 3678 2168 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3806 5016 3806 Size: 1210 4704 BclI T'GATC_A Cuts at: 1548 1548 Size: 5914 BfrBI ATG'CAT Cuts at: 3035 3035 Size: 5914 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4525 136 Size: 107 122 71 631 605 2853 1525 Fragments arranged by size: 2853 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5909 5909 Size: 5914 BlpI GC'TnA_GC Cuts at: 3331 3331 Size: 5914 Bme1580I G_kGCm'C Cuts at: 1790 3975 5131 1790 Size: 2185 1156 2573 Fragments arranged by size: 2573 2185 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5914 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3123 3645 4603 Size: 416 53 83 399 24 1706 522 958 Cuts at: 4603 442 Size: 1753 Fragments arranged by size: 1753 1706 958 522 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5914 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2261 2778 4574 1239 Size: 1022 517 1796 2579 Fragments arranged by size: 2579 1796 1022 517 Bpu10I CC'TnA_GC Cuts at: 2910 2910 Size: 5914 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2300 2429 4039 4904 5202 2300 Size: 129 1610 865 298 3012 Fragments arranged by size: 3012 1610 865 298 129 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4577 882 Size: 617 3078 2219 Fragments arranged by size: 3078 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3705 493 Size: 711 2501 2702 Fragments arranged by size: 2702 2501 711 BsaBI GATnn'nnATC Cuts at: 3156 5908 3156 Size: 2752 3162 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2889 3721 4103 4738 Size: 53 83 186 936 1356 832 382 635 Cuts at: 4738 275 Size: 1451 Fragments arranged by size: 1451 1356 936 832 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4347 4745 5019 5166 5577 4347 Size: 398 274 147 411 4684 Fragments arranged by size: 4684 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2152 2182 2751 2781 1178 Size: 30 165 30 749 30 569 30 4311 Fragments arranged by size: 4311 749 569 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2032 2288 2867 1186 Size: 846 256 579 4233 Fragments arranged by size: 4233 846 579 256 BseYI C'CCAG_C Cuts at: 5117 5613 5117 Size: 496 5418 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5341 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3364 4125 5153 1540 Size: 275 186 1363 761 1028 2301 Fragments arranged by size: 2301 1363 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2063 2106 2877 3975 4060 5131 2063 Size: 43 771 1098 85 1071 2846 Fragments arranged by size: 2846 1098 1071 771 85 43 BsmI GAATG_Cn' Cuts at: 3425 3518 3425 Size: 93 5821 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2106 2877 3233 3975 4060 5131 Size: 399 273 43 771 356 742 85 1071 Cuts at: 5131 1391 Size: 2174 Fragments arranged by size: 2174 1071 771 742 399 356 273 85 43 BspEI T'CCGG_A Cuts at: 5577 5577 Size: 5914 BspHI T'CATG_A Cuts at: 3803 5533 3803 Size: 1730 4184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3269 844 Size: 2425 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3801 1394 Size: 429 1978 3507 Fragments arranged by size: 3507 1978 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2248 4409 4583 65 Size: 1183 1000 2161 174 1396 Fragments arranged by size: 2161 1396 1183 1000 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2011 2231 4558 4745 1700 Size: 110 201 220 2327 187 2869 Fragments arranged by size: 2869 2327 220 201 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5914 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5914 BssSI C'ACGA_G Cuts at: 3974 4986 3974 Size: 1012 4902 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5914 BstBI TT'CG_AA Cuts at: 4718 4718 Size: 5914 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5914 BstXI CCAn_nnnn'nTGG Cuts at: 2441 2441 Size: 5914 BstYI r'GATC_y Cuts at: 1972 2208 2587 3244 3611 3996 4013 5465 5551 Size: 236 379 657 367 385 17 1452 86 Cuts at: 5551 5563 5909 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 657 385 379 367 346 236 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2499 2499 Size: 5914 BtgI C'CryG_G Cuts at: 513 1065 2004 2035 2054 513 Size: 552 939 31 19 4373 Fragments arranged by size: 4373 939 552 31 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2933 487 Size: 683 56 1707 3468 Fragments arranged by size: 3468 1707 683 56 BtsI GCAGTG_nn' Cuts at: 3430 4224 4244 3430 Size: 794 20 5100 Fragments arranged by size: 5100 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5914 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5879 DraI TTT'AAA Cuts at: 3218 3218 Size: 5914 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4921 809 Size: 507 3605 1802 Fragments arranged by size: 3605 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2233 2359 2937 Size: 54 1425 50 275 18 403 126 578 Cuts at: 2937 3361 4250 5879 8 Size: 424 889 1629 43 Fragments arranged by size: 1629 1425 889 578 424 403 275 126 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3361 1537 Size: 275 1549 4090 Fragments arranged by size: 4090 1549 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2343 3844 1353 Size: 990 1501 3423 Fragments arranged by size: 3423 1501 990 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2099 2240 4501 4875 5021 387 Size: 1064 138 510 141 2261 374 146 1280 Fragments arranged by size: 2261 1280 1064 510 374 146 141 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 2646 2778 3977 4574 5361 5629 Size: 393 629 385 132 1199 597 787 268 Cuts at: 5629 1239 Size: 1524 Fragments arranged by size: 1524 1199 787 629 597 393 385 268 132 EcoNI CCTnn'n_nnAGG Cuts at: 2084 2225 2882 2084 Size: 141 657 5116 Fragments arranged by size: 5116 657 141 EcoRI G'AATT_C Cuts at: 3223 3601 3223 Size: 378 5536 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2651 2651 Size: 5914 FseI GG_CCGG'CC Cuts at: 2235 2235 Size: 5914 FspI TGC'GCA Cuts at: 2174 4420 2174 Size: 2246 3668 HaeII r_GCGC'y Cuts at: 1678 2929 5061 1678 Size: 1251 2132 2531 Fragments arranged by size: 2531 2132 1251 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2604 2636 2762 2794 2841 Size: 32 135 32 1032 32 126 32 47 Cuts at: 2841 2873 4555 4587 4629 4661 1373 Size: 32 1682 32 42 32 2626 Fragments arranged by size: 2626 1682 1032 135 126 47 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3258 3504 669 Size: 988 281 1320 246 3079 Fragments arranged by size: 3079 1320 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5914 HpaI GTT'AAC Cuts at: 3504 3504 Size: 5914 KpnI G_GTAC'C Cuts at: 3239 3239 Size: 5914 MfeI C'AATT_G Cuts at: 3513 3513 Size: 5914 MluI A'CGCG_T Cuts at: 5790 5790 Size: 5914 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2812 5028 5212 1305 Size: 1507 2216 184 2007 Fragments arranged by size: 2216 2007 1507 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2361 10 Size: 54 1425 343 529 3563 Fragments arranged by size: 3563 1425 529 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2066 3872 4231 4390 518 Size: 648 900 1806 359 159 2042 Fragments arranged by size: 2042 1806 900 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2037 2056 3330 4007 5155 5400 1606 Size: 431 19 1274 677 1148 245 2120 Fragments arranged by size: 2120 1274 1148 677 431 245 19 NaeI GCC'GGC Cuts at: 2233 2233 Size: 5914 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4423 Fragments arranged by size: 4423 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5914 NgoMIV G'CCGG_C Cuts at: 2231 2231 Size: 5914 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5914 NotI GC'GGCC_GC Cuts at: 3361 3361 Size: 5914 NsiI A_TGCA'T Cuts at: 3037 3037 Size: 5914 NspI r_CATG'y Cuts at: 1132 1229 2982 3272 4817 1132 Size: 97 1753 290 1545 2229 Fragments arranged by size: 2229 1753 1545 290 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5914 PciI A'CATG_T Cuts at: 4813 4813 Size: 5914 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5914 PmeI GTTT'AAAC Cuts at: 3218 3218 Size: 5914 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3983 4015 5496 5528 3983 Size: 32 1481 32 4369 Fragments arranged by size: 4369 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5914 PsiI TTA'TAA Cuts at: 2679 3484 2679 Size: 805 5109 PstI C_TGCA'G Cuts at: 830 2715 3266 830 Size: 1885 551 3478 Fragments arranged by size: 3478 1885 551 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5914 SacII CC_GC'GG Cuts at: 2038 2057 2038 Size: 19 5895 SalI G'TCGA_C Cuts at: 1936 3256 1936 Size: 1320 4594 SbfI CC_TGCA'GG Cuts at: 3266 3266 Size: 5914 ScaI AGT'ACT Cuts at: 4162 5874 4162 Size: 1712 4202 SfcI C'TryA_G Cuts at: 826 1045 2711 3262 4397 5078 5269 826 Size: 219 1666 551 1135 681 191 1471 Fragments arranged by size: 1666 1471 1135 681 551 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5914 SmaI CCC'GGG Cuts at: 1872 3241 1872 Size: 1369 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2279 2408 4018 4919 5181 820 Size: 197 926 336 129 1610 901 262 1553 Fragments arranged by size: 1610 1553 926 901 336 262 197 129 SnaBI TAC'GTA Cuts at: 493 3705 493 Size: 3212 2702 SpeI A'CTAG_T Cuts at: 152 2384 152 Size: 2232 3682 SphI G_CATG'C Cuts at: 1132 2982 3272 1132 Size: 1850 290 3774 Fragments arranged by size: 3774 1850 290 SspI AAT'ATT Cuts at: 5 52 3838 5 Size: 47 3786 2081 Fragments arranged by size: 3786 2081 47 StuI AGG'CCT Cuts at: 1517 2220 1517 Size: 703 5211 StyI C'CwwG_G Cuts at: 513 1065 2004 2121 2440 3012 3353 513 Size: 552 939 117 319 572 341 3074 Fragments arranged by size: 3074 939 572 552 341 319 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3941 3958 4111 4296 4741 325 Size: 1255 2361 17 153 185 445 1498 Fragments arranged by size: 2361 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4160 5872 96 Size: 274 80 33 51 1443 2183 1712 138 Fragments arranged by size: 2183 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2152 2184 2451 2483 2751 2783 5874 5906 2152 Size: 32 267 32 268 32 3091 32 2160 Fragments arranged by size: 3091 2160 268 267 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5914 XbaI T'CTAG_A Cuts at: 3250 3250 Size: 5914 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5914 XmaI C'CCGG_G Cuts at: 1870 3239 1870 Size: 1369 4545 XmnI GAAnn'nnTTC Cuts at: 2417 4043 2417 Size: 1626 4288 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2890 3722 276 Size: 53 83 186 936 1356 832 2468 Fragments arranged by size: 2468 1356 936 832 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI