(Linear) (Six-base) MAPSORT of: KIEE3171.seq Check: 3643 from: 1 to: 1209 KIEE3171.orf With 182 enzymes: SgfI * December 27, 2011 16:34 .. AatII G_ACGT'C Cuts at: 0 887 1209 Size: 887 322 AccI GT'mk_AC Cuts at: 0 44 1209 Size: 44 1165 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 641 1209 Size: 641 568 AhdI GACnn_n'nnGTC Cuts at: 0 771 1209 Size: 771 438 AleI CACnn'nnGTG Cuts at: 0 61 1209 Size: 61 1148 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 193 1209 Size: 193 1016 ApoI r'AATT_y Cuts at: 0 591 1209 Size: 591 618 AvaI C'yCGr_G Cuts at: 0 816 1209 Size: 816 393 BamHI G'GATC_C Cuts at: 0 582 1209 Size: 582 627 BbsI GAAGACnn'nnnn_ Cuts at: 0 904 1209 Size: 904 305 BbvCI CC'TCA_GC Cuts at: 0 905 1209 Size: 905 304 BfrBI ATG'CAT Cuts at: 0 1030 1209 Size: 1030 179 BmrI ACTGGGnnnn_n' Cuts at: 0 1118 1209 Size: 1118 91 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 773 1209 Size: 256 517 436 Fragments arranged by size: 517 436 256 Bpu10I CC'TnA_GC Cuts at: 0 905 1209 Size: 905 304 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 424 1209 Size: 295 129 785 Fragments arranged by size: 785 295 129 BsaBI GATnn'nnATC Cuts at: 0 1151 1209 Size: 1151 58 BsaHI Gr'CG_yC Cuts at: 0 884 1209 Size: 884 325 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 147 177 746 776 1209 Size: 147 30 569 30 433 Fragments arranged by size: 569 433 147 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 27 283 862 1209 Size: 27 256 579 347 Fragments arranged by size: 579 347 256 27 BsiHKAI G_wGCw'C Cuts at: 0 58 101 872 1209 Size: 58 43 771 337 Fragments arranged by size: 771 337 58 43 Bsp1286I G_dGCh'C Cuts at: 0 58 101 872 1209 Size: 58 43 771 337 Fragments arranged by size: 771 337 58 43 BsrDI GCAATG_nn' Cuts at: 0 243 1209 Size: 243 966 BsrFI r'CCGG_y Cuts at: 0 6 226 1209 Size: 6 220 983 Fragments arranged by size: 983 220 6 BstXI CCAn_nnnn'nTGG Cuts at: 0 436 1209 Size: 436 773 BstYI r'GATC_y Cuts at: 0 203 582 1209 Size: 203 379 627 Fragments arranged by size: 627 379 203 Bsu36I CC'TnA_GG Cuts at: 0 494 1209 Size: 494 715 BtgI C'CryG_G Cuts at: 0 30 49 1209 Size: 30 19 1160 Fragments arranged by size: 1160 30 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 928 1209 Size: 928 281 EaeI y'GGCC_r Cuts at: 0 228 354 932 1209 Size: 228 126 578 277 Fragments arranged by size: 578 277 228 126 EarI CTCTTCn'nnn_ Cuts at: 0 338 1209 Size: 338 871 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 94 235 1209 Size: 94 141 974 Fragments arranged by size: 974 141 94 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 641 773 1209 Size: 256 385 132 436 Fragments arranged by size: 436 385 256 132 EcoNI CCTnn'n_nnAGG Cuts at: 0 79 220 877 1209 Size: 79 141 657 332 Fragments arranged by size: 657 332 141 79 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 646 1209 Size: 646 563 FseI GG_CCGG'CC Cuts at: 0 230 1209 Size: 230 979 FspI TGC'GCA Cuts at: 0 169 1209 Size: 169 1040 HaeII r_GCGC'y Cuts at: 0 924 1209 Size: 924 285 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 599 631 757 789 836 868 1209 Size: 599 32 126 32 47 32 341 Fragments arranged by size: 599 341 126 47 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 807 1209 Size: 807 402 MscI TGG'CCA Cuts at: 0 356 1209 Size: 356 853 MslI CAynn'nnrTG Cuts at: 0 61 1209 Size: 61 1148 MspA1I CmG'CkG Cuts at: 0 32 51 1209 Size: 32 19 1158 Fragments arranged by size: 1158 32 19 NaeI GCC'GGC Cuts at: 0 228 1209 Size: 228 981 NgoMIV G'CCGG_C Cuts at: 0 226 1209 Size: 226 983 NsiI A_TGCA'T Cuts at: 0 1032 1209 Size: 1032 177 NspI r_CATG'y Cuts at: 0 977 1209 Size: 977 232 PsiI TTA'TAA Cuts at: 0 674 1209 Size: 674 535 PstI C_TGCA'G Cuts at: 0 710 1209 Size: 710 499 SacII CC_GC'GG Cuts at: 0 33 52 1209 Size: 33 19 1157 Fragments arranged by size: 1157 33 19 SfcI C'TryA_G Cuts at: 0 706 1209 Size: 706 503 SmlI C'TyrA_G Cuts at: 0 274 403 1209 Size: 274 129 806 Fragments arranged by size: 806 274 129 SpeI A'CTAG_T Cuts at: 0 379 1209 Size: 379 830 SphI G_CATG'C Cuts at: 0 977 1209 Size: 977 232 StuI AGG'CCT Cuts at: 0 215 1209 Size: 215 994 StyI C'CwwG_G Cuts at: 0 116 435 1007 1209 Size: 116 319 572 202 Fragments arranged by size: 572 319 202 116 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 147 179 446 478 746 778 1209 Size: 147 32 267 32 268 32 431 Fragments arranged by size: 431 268 267 147 32 32 32 XmnI GAAnn'nnTTC Cuts at: 0 412 1209 Size: 412 797 ZraI GAC'GTC Cuts at: 0 885 1209 Size: 885 324 Enzymes that do cut: AatII AccI AcuI AhdI AleI AlfI ApoI AvaI BamHI BbsI BbvCI BfrBI BmrI BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI BsiHKAI Bsp1286I BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtgZI EaeI EarI EciI Eco57MI EcoNI FalI FseI FspI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NgoMIV NsiI NspI PsiI PstI SacII SfcI SmlI SpeI SphI StuI StyI TstI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AflIII AgeI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaWI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoO109I EcoRI EcoRV FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI