################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HhaI HinP1I HpaI HpaII KasI KpnI KspI MamI MluI MluNI MroI MscI MspA1I MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI StyI Tth111I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 534 AAATTT [GA]^AATT[CT] AcyI 39 GGCGTC G[GA]^CG[CT]C AlwI 841 GATCC GGATC(4/5) ApoI 534 AAATTT [GA]^AATT[CT] AspHI 634 GAGCTC G[AT]GC[AT]^C BanI 117 GGTGCC G^G[CT][GA]CC BglII 770 AGATCT A^GATCT BpmI 936 CTGGAG CTGGAG(16/14) BsaHI 39 GGCGTC G[GA]^CG[CT]C BsaJI 377 CCACGG C^C[ACGT][ACGT]GG BseRI 899 GAGGAG GAGGAG(10/8) BsiHKAI 634 GAGCTC G[AT]GC[AT]^C Bsp1407I 1074 TGTACA T^GTACA BsrGI 1074 TGTACA T^GTACA BssSI 61 CACGAG CACGAG(-5/-1) BstAPI 138 GCACTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 579 CCATTTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 770 AGATCT [GA]^GATC[CT] DraI 582 TTTAAA TTT^AAA DsaI 377 CCACGG C^C[GA][CT]GG FauI 135 CCCGC CCCGC(4/6) HaeIII 21 GGCC GG^CC HgaI 40 GCGTC GACGC(5/10) Hin1I 39 GGCGTC G[GA]^CG[CT]C HincII 736 GTTGAC GT[CT]^[GA]AC HindII 736 GTTGAC GT[CT]^[GA]AC HindIII 611 AAGCTT A^AGCTT HphI 199 TCACC GGTGA(8/7) MaeII 252 ACGT A^CGT MfeI 292 CAATTG C^AATTG MflI 770 AGATCT [GA]^GATC[CT] MunI 292 CAATTG C^AATTG NlaIV 117 GGTGCC GG[ACGT]^[ACGT]CC NspI 717 GCATGT [GA]CATG^[CT] PflMI 108 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 564 GACTC GAGTC(4/5) SacI 634 GAGCTC GAGCT^C SapI 484 GAAGAGC GCTCTTC(1/4) SmlI 16 CTCAAG C^T[CT][GA]AG SspBI 1074 TGTACA T^GTACA SspI 543 AATATT AAT^ATT SwaI 581 ATTTAAAT ATTT^AAAT Tsp45I 406 GTGAC ^GT[GC]AC Van91I 108 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 255 TCTAGA T^CTAGA XhoII 770 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AfaI 1075 GTAC GT^AC 1101 GTAC AlwNI 109 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG 593 CAGTGTCTG BsmFI 391 GGGAC GGGAC(10/14) 641 GGGAC BsrI 713 ACTGG ACTGG(1/-1) 108 CCAGT BstF5I 348 GGATG GGATG(2/0) 513 GGATG Cac8I 134 GCCCGC GC[ACGT]^[ACGT]GC 609 GCAAGC FokI 348 GGATG GGATG(9/13) 513 GGATG MaeIII 406 GTGAC ^GT[ACGT]AC 864 GTTAC PstI 318 CTGCAG CTGCA^G 727 CTGCAG RsaI 1075 GTAC GT^AC 1101 GTAC SfaNI 357 GATGC GCATC(5/9) 74 GCATC SfcI 318 CTGCAG C^T[GA][CT]AG 727 CTGCAG TaqI 50 TCGA T^CGA 855 TCGA TthHB8I 50 TCGA T^CGA 855 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AluI BanII BbsI BbvI BfaI BmyI BpuAI BsgI BsiYI BslI Bsp1286I BssKI BstNI DdeI DpnII EarI EcoRII Fnu4HI HinfI ItaI Ksp632I MaeI MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII Sau3AI ScrFI TfiI Tru9I TseI Tsp509I TspRI