(Circular) (Six-base) MAPSORT of: pFN21AE3156.seq Check: 5538 from: 1 to: 5830 pFN21AE3156 5830 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3173 1937 Size: 1236 4594 Acc65I G'GTAC_C Cuts at: 3151 3151 Size: 5830 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 2247 2400 2469 2604 2756 3893 5277 Size: 422 193 153 69 135 152 1137 1384 Cuts at: 5277 5545 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1137 422 268 193 153 152 135 69 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 4729 5706 1203 Size: 3526 977 1327 Fragments arranged by size: 3526 1327 977 AgeI A'CCGG_T Cuts at: 4661 4661 Size: 5830 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5830 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3000 1184 Size: 1816 4014 AlwNI CAG_nnn'CTG Cuts at: 2119 2603 3573 5145 2119 Size: 484 970 1572 2804 Fragments arranged by size: 2804 1572 970 484 ApaLI G'TGCA_C Cuts at: 3887 5043 3887 Size: 1156 4674 ApoI r'AATT_y Cuts at: 1493 2539 3139 3365 3517 5626 1493 Size: 1046 600 226 152 2109 1697 Fragments arranged by size: 2109 1697 1046 600 226 152 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 3155 1870 Size: 73 1212 4545 Fragments arranged by size: 4545 1212 73 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 3151 4606 618 Size: 325 217 627 335 1029 1455 1842 Fragments arranged by size: 1842 1455 1029 627 335 325 217 BanII G_rGCy'C Cuts at: 1391 2022 2643 3058 3149 1391 Size: 631 621 415 91 4072 Fragments arranged by size: 4072 631 621 415 91 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2235 2466 2573 928 Size: 971 336 231 107 4185 Fragments arranged by size: 4185 971 336 231 107 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4021 4055 1903 Size: 34 2084 34 3678 Fragments arranged by size: 3678 2084 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3722 4932 3722 Size: 1210 4620 BclI T'GATC_A Cuts at: 1548 1548 Size: 5830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4441 136 Size: 107 122 71 631 605 2769 1525 Fragments arranged by size: 2769 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2775 5825 2775 Size: 3050 2780 BlpI GC'TnA_GC Cuts at: 3247 3247 Size: 5830 Bme1580I G_kGCm'C Cuts at: 1790 3891 5047 1790 Size: 2101 1156 2573 Fragments arranged by size: 2573 2101 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5830 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3561 4519 442 Size: 416 53 83 399 24 2144 958 1753 Fragments arranged by size: 2144 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2962 4490 1239 Size: 1723 1528 2579 Fragments arranged by size: 2579 1723 1528 Bpu10I CC'TnA_GC Cuts at: 2343 2642 2343 Size: 299 5531 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2006 3955 4820 5118 2006 Size: 1949 865 298 2718 Fragments arranged by size: 2718 1949 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4493 882 Size: 617 2994 2219 Fragments arranged by size: 2994 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3621 493 Size: 711 2417 2702 Fragments arranged by size: 2702 2417 711 BsaBI GATnn'nnATC Cuts at: 5824 5824 Size: 5830 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2045 3637 4019 4654 Size: 53 83 186 936 512 1592 382 635 Cuts at: 4654 275 Size: 1451 Fragments arranged by size: 1592 1451 936 635 512 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4263 4661 4935 5082 5493 4263 Size: 398 274 147 411 4600 Fragments arranged by size: 4600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2151 2181 2877 2907 1178 Size: 30 165 30 748 30 696 30 4101 Fragments arranged by size: 4101 748 696 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2919 1186 Size: 1733 4097 BseYI C'CCAG_C Cuts at: 5033 5529 5033 Size: 496 5334 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2090 2616 3035 1163 Size: 573 354 526 419 3958 Fragments arranged by size: 3958 573 526 419 354 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3280 4041 5069 1540 Size: 275 186 1279 761 1028 2301 Fragments arranged by size: 2301 1279 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2643 3891 3976 5047 2643 Size: 1248 85 1071 3426 Fragments arranged by size: 3426 1248 1071 85 BsmI GAATG_Cn' Cuts at: 3341 3434 3341 Size: 93 5737 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2643 3058 3149 3891 3976 5047 Size: 399 232 621 415 91 742 85 1071 Cuts at: 5047 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 621 415 399 232 91 85 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 3719 5449 3719 Size: 1730 4100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3185 844 Size: 2341 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3717 1394 Size: 429 1894 3507 Fragments arranged by size: 3507 1894 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4325 4499 65 Size: 1183 3077 174 1396 Fragments arranged by size: 3077 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4474 4661 1700 Size: 110 2664 187 2869 Fragments arranged by size: 2869 2664 187 110 BsrGI T'GTAC_A Cuts at: 96 3079 96 Size: 2983 2847 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 2066 3890 4902 2066 Size: 1824 1012 2994 Fragments arranged by size: 2994 1824 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2149 794 Size: 1355 4475 BstBI TT'CG_AA Cuts at: 4634 4634 Size: 5830 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5830 BstXI CCAn_nnnn'nTGG Cuts at: 2591 2591 Size: 5830 BstYI r'GATC_y Cuts at: 1972 2775 3160 3527 3912 3929 5381 5467 5479 Size: 803 385 367 385 17 1452 86 12 Cuts at: 5479 5825 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 803 385 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2382 513 Size: 552 1317 3961 Fragments arranged by size: 3961 1317 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5091 Fragments arranged by size: 5091 683 56 BtsI GCAGTG_nn' Cuts at: 2107 2170 2604 2953 3346 4140 4160 2107 Size: 63 434 349 393 794 20 3777 Fragments arranged by size: 3777 794 434 393 349 63 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2285 2320 529 Size: 35 1721 35 4039 Fragments arranged by size: 4039 1721 35 35 DraI TTT'AAA Cuts at: 2589 3134 2589 Size: 545 5285 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4837 809 Size: 507 3521 1802 Fragments arranged by size: 3521 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3277 4166 5795 Size: 54 1425 50 275 18 1447 889 1629 Cuts at: 5795 8 Size: 43 Fragments arranged by size: 1629 1447 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3277 1537 Size: 275 1465 4090 Fragments arranged by size: 4090 1465 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2418 2433 2484 3760 1353 Size: 1065 15 51 1276 3423 Fragments arranged by size: 3423 1276 1065 51 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4417 4791 4937 387 Size: 1064 138 2828 374 146 1280 Fragments arranged by size: 2828 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2641 2641 Size: 5830 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2247 2400 2469 2604 2756 2962 Size: 393 422 193 153 69 135 152 206 Cuts at: 2962 3893 4490 5277 5545 1239 Size: 931 597 787 268 1524 Fragments arranged by size: 1524 931 787 597 422 393 268 206 193 153 152 135 69 EcoRI G'AATT_C Cuts at: 3139 3517 3139 Size: 378 5452 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2851 2851 Size: 5830 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 4977 1678 Size: 3299 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2064 2096 2353 2385 4471 Size: 32 135 32 492 32 257 32 2086 Cuts at: 4471 4503 4545 4577 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2086 492 257 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2743 3174 3420 669 Size: 988 281 805 431 246 3079 Fragments arranged by size: 3079 988 805 431 281 246 HindIII A'AGCT_T Cuts at: 748 2616 748 Size: 1868 3962 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KpnI G_GTAC'C Cuts at: 3155 3155 Size: 5830 MfeI C'AATT_G Cuts at: 2297 3429 2297 Size: 1132 4698 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2069 4944 5128 1305 Size: 764 2875 184 2007 Fragments arranged by size: 2875 2007 764 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4008 Fragments arranged by size: 4008 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2209 2352 2589 3788 4147 4306 Size: 648 843 200 143 237 1199 359 159 Cuts at: 4306 518 Size: 2042 Fragments arranged by size: 2042 1199 843 648 359 237 200 159 143 MspA1I CmG'CkG Cuts at: 1606 3246 3923 5071 5316 1606 Size: 1640 677 1148 245 2120 Fragments arranged by size: 2120 1640 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5278 NdeI CA'TA_TG Cuts at: 387 387 Size: 5830 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5830 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NspI r_CATG'y Cuts at: 1132 1229 2726 3188 4733 1132 Size: 97 1497 462 1545 2229 Fragments arranged by size: 2229 1545 1497 462 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5830 PciI A'CATG_T Cuts at: 4729 4729 Size: 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 2119 1108 Size: 1011 4819 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2021 2053 3899 3931 5412 5444 2021 Size: 32 1846 32 1481 32 2407 Fragments arranged by size: 2407 1846 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5830 PsiI TTA'TAA Cuts at: 2670 3400 2670 Size: 730 5100 PstI C_TGCA'G Cuts at: 830 2327 2736 3182 830 Size: 1497 409 446 3478 Fragments arranged by size: 3478 1497 446 409 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 SacI G_AGCT'C Cuts at: 2643 2643 Size: 5830 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SapI GCTCTTCn'nnn_ Cuts at: 2484 2484 Size: 5830 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 4078 5790 4078 Size: 1712 4118 SfcI C'TryA_G Cuts at: 826 1045 2323 2732 3178 4313 4994 5185 826 Size: 219 1278 409 446 1135 681 191 1471 Fragments arranged by size: 1471 1278 1135 681 446 409 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 3157 1872 Size: 1285 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2021 3934 4835 5097 820 Size: 197 926 78 1913 901 262 1553 Fragments arranged by size: 1913 1553 926 901 262 197 78 SnaBI TAC'GTA Cuts at: 493 3621 493 Size: 3128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 3188 1132 Size: 2056 3774 SspI AAT'ATT Cuts at: 5 52 2550 3754 5 Size: 47 2498 1204 2081 Fragments arranged by size: 2498 2081 1204 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5830 StyI C'CwwG_G Cuts at: 513 1065 3269 513 Size: 552 2204 3074 Fragments arranged by size: 3074 2204 552 SwaI ATTT'AAAT Cuts at: 2589 2589 Size: 5830 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2888 2956 3857 3874 4027 4212 4657 Size: 1255 1308 68 901 17 153 185 445 Cuts at: 4657 325 Size: 1498 Fragments arranged by size: 1498 1308 1255 901 445 185 153 68 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3079 4076 5788 Size: 274 80 33 51 1443 1102 997 1712 Cuts at: 5788 96 Size: 138 Fragments arranged by size: 1712 1443 1102 997 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2151 2183 5790 5822 2151 Size: 32 3607 32 2159 Fragments arranged by size: 3607 2159 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5830 XbaI T'CTAG_A Cuts at: 2260 3166 2260 Size: 906 4924 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5830 XmaI C'CCGG_G Cuts at: 1870 3155 1870 Size: 1285 4545 XmnI GAAnn'nnTTC Cuts at: 3959 3959 Size: 5830 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI