################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AscI Asp700I AspEI AspI AvaII AviII AvrII BalI BamHI BbrPI BbvCI BcgI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiWI BspDI BspEI BspHI BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI Eam1105I EarI Eco81I EcoO65I EcoRI EcoT22I FseI FspI HindIII HpaI Ksp632I KspI MaeII MamI MluI MluNI MroI MscI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 1238 GGTACC G^GTACC AccI 1601 GTCGAC GT^[AC][GT]AC AflIII 1346 ACATGT A^C[GA][CT]GT Aor51HI 214 AGCGCT AGC^GCT ApaI 223 GGGCCC GGGCC^C AseI 490 ATTAAT AT^TAAT AsnI 490 ATTAAT AT^TAAT Asp718I 1238 GGTACC G^GTACC BciVI 347 GTATCC GTATCC(6/5) BclI 743 TGATCA T^GATCA BsiEI 314 CGGCCG CG[GA][CT]^CG BsmBI 193 GAGACG CGTCTC(1/5) BsmI 1497 GAATGC GAATGC(1/-1) Bsp1407I 1418 TGTACA T^GTACA BspLU11I 1346 ACATGT A^CATGT BsrBI 169 GAGCGG CCGCTC(-3/-3) BsrGI 1418 TGTACA T^GTACA BstXI 653 CCAAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 222 GGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 334 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1132 CCATGG C^C[GA][CT]GG EaeI 314 CGGCCG [CT]^GGCC[GA] EagI 314 CGGCCG C^GGCCG EclXI 314 CGGCCG C^GGCCG Eco47III 214 AGCGCT AGC^GCT Eco52I 314 CGGCCG C^GGCCG EcoNI 1371 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 222 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoRV 709 GATATC GAT^ATC EcoT14I 1132 CCATGG C^C[AT][AT]GG FbaI 743 TGATCA T^GATCA HgaI 158 GCGTC GACGC(5/10) HphI 1311 GGTGA GGTGA(8/7) KpnI 1238 GGTACC GGTAC^C MspA1I 63 CAGCGG C[AC]G^C[GT]G NcoI 1132 CCATGG C^CATGG PshBI 490 ATTAAT AT^TAAT SalI 1601 GTCGAC G^TCGAC SmaI 148 CCCGGG CCC^GGG SspBI 1418 TGTACA T^GTACA SspI 641 AATATT AAT^ATT StyI 1132 CCATGG C^C[AT][AT]GG Tsp45I 1045 GTCAC ^GT[GC]AC XbaI 1491 TCTAGA T^CTAGA XmaCI 148 CCCGGG C^CCGGG XmaI 148 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AvaI 148 CCCGGG C^[CT]CG[GA]G 1515 CTCGGG BanII 145 GAGCCC G[GA]GC[CT]^C 223 GGGCCC BbeI 67 GGCGCC GGCGC^C 899 GGCGCC BbsI 88 GAAGAC GAAGAC(2/6) 1429 GTCTTC BfaI 1220 CTAG C^TAG 1492 CTAG BpmI 460 CTGGAG CTGGAG(16/14) 633 CTGGAG BpuAI 88 GAAGAC GAAGAC(2/6) 1429 GTCTTC BsgI 48 GTGCAG GTGCAG(16/14) 1363 GTGCAG BsmAI 193 GAGAC GTCTC(1/5) 507 GTCTC BsmFI 173 GGGAC GGGAC(10/14) 1031 GTCCC BsoBI 148 CCCGGG C^[CT]CG[GA]G 1515 CTCGGG BstAPI 1198 GCAAGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1275 GCAGAGCCTGC Cac8I 266 GCGGGC GC[ACGT]^[ACGT]GC 1280 GCCTGC HincII 358 GTCAAC GT[CT]^[GA]AC 1601 GTCGAC HindII 358 GTCAAC GT[CT]^[GA]AC 1601 GTCGAC KasI 67 GGCGCC G^GCGCC 899 GGCGCC MaeI 1220 CTAG C^TAG 1492 CTAG NarI 67 GGCGCC GG^CGCC 899 GGCGCC PleI 4 GACTC GAGTC(4/5) 1599 GAGTC PstI 1231 CTGCAG CTGCA^G 1552 CTGCAG SfcI 1231 CTGCAG C^T[GA][CT]AG 1552 CTGCAG SfoI 67 GGCGCC GGC^GCC 899 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AspHI BanI BbvI BcnI BglII BmyI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstYI CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspI MunI MvaI MwoI NciI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII