(Circular) (Six-base) MAPSORT of: pFN21AE4140.seq Check: 3377 from: 1 to: 6376 pFN21AE4140 6376 bp With 182 enzymes: SgfI * March 28, 2012 13:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4186 278 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3607 3719 1937 Size: 1670 112 4594 Fragments arranged by size: 4594 1670 112 Acc65I G'GTAC_C Cuts at: 3243 3697 3243 Size: 454 5922 AclI AA'CG_TT Cuts at: 1563 4503 4876 1563 Size: 2940 373 3063 Fragments arranged by size: 3063 2940 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3398 4439 5823 6091 1632 Size: 1766 1041 1384 268 1917 Fragments arranged by size: 1917 1766 1384 1041 268 AfeI AGC'GCT Cuts at: 2221 2221 Size: 6376 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6179 AflIII A'CryG_T Cuts at: 1203 3351 5275 6252 1203 Size: 2148 1924 977 1327 Fragments arranged by size: 2148 1924 1327 977 AgeI A'CCGG_T Cuts at: 5207 5207 Size: 6376 AhdI GACnn_n'nnGTC Cuts at: 5105 5105 Size: 6376 AleI CACnn'nnGTG Cuts at: 1166 3512 1166 Size: 2346 4030 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3133 1184 Size: 1949 4427 AlwNI CAG_nnn'CTG Cuts at: 3286 3376 3597 4119 5691 3286 Size: 90 221 522 1572 3971 Fragments arranged by size: 3971 1572 522 221 90 ApaI G_GGCC'C Cuts at: 2232 2232 Size: 6376 ApaLI G'TGCA_C Cuts at: 4433 5589 4433 Size: 1156 5220 ApoI r'AATT_y Cuts at: 1493 3685 3911 4063 6172 1493 Size: 2192 226 152 2109 1697 Fragments arranged by size: 2192 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2496 4930 160 Size: 872 1464 2434 1606 Fragments arranged by size: 2434 1606 1464 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 AvaI C'yCGr_G Cuts at: 1870 1943 2153 3520 3701 1870 Size: 73 210 1367 181 4545 Fragments arranged by size: 4545 1367 210 181 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3154 3187 3154 Size: 33 6343 BamHI G'GATC_C Cuts at: 3706 4073 3706 Size: 367 6009 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 2904 3243 3697 5152 Size: 325 217 627 285 832 339 454 1455 Cuts at: 5152 618 Size: 1842 Fragments arranged by size: 1842 1455 832 627 454 339 325 285 217 BanII G_rGCy'C Cuts at: 1391 2154 2232 3695 1391 Size: 763 78 1463 4072 Fragments arranged by size: 4072 1463 763 78 BbeI G_GCGC'C Cuts at: 2076 2908 2076 Size: 832 5544 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2100 3427 928 Size: 971 201 1327 3877 Fragments arranged by size: 3877 1327 971 201 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4567 4601 1903 Size: 34 2630 34 3678 Fragments arranged by size: 3678 2630 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2363 4268 5478 2363 Size: 1905 1210 3261 Fragments arranged by size: 3261 1905 1210 BclI T'GATC_A Cuts at: 1548 2748 1548 Size: 1200 5176 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4987 136 Size: 107 122 71 631 605 3315 1525 Fragments arranged by size: 3315 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2565 3493 3652 6371 2565 Size: 928 159 2719 2570 Fragments arranged by size: 2719 2570 928 159 BlpI GC'TnA_GC Cuts at: 3793 3793 Size: 6376 Bme1580I G_kGCm'C Cuts at: 1790 2232 4437 5593 1790 Size: 442 2205 1156 2573 Fragments arranged by size: 2573 2205 1156 442 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6376 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4107 5065 442 Size: 416 53 83 399 24 2690 958 1753 Fragments arranged by size: 2690 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6376 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3535 3535 Size: 6376 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2486 2659 5036 1239 Size: 1247 173 2377 2579 Fragments arranged by size: 2579 2377 1247 173 Bpu10I CC'TnA_GC Cuts at: 2408 3312 2408 Size: 904 5472 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4501 5366 5664 4501 Size: 865 298 5213 Fragments arranged by size: 5213 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5039 882 Size: 617 3540 2219 Fragments arranged by size: 3540 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4167 493 Size: 711 2963 2702 Fragments arranged by size: 2963 2702 711 BsaBI GATnn'nnATC Cuts at: 6370 6370 Size: 6376 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 2163 2905 4183 Size: 53 83 186 936 540 90 742 1278 Cuts at: 4183 4565 5200 275 Size: 382 635 1451 Fragments arranged by size: 1451 1278 936 742 635 540 382 186 90 83 53 BsaWI w'CCGG_w Cuts at: 4809 5207 5481 5628 6039 4809 Size: 398 274 147 411 5146 Fragments arranged by size: 5146 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6151 Fragments arranged by size: 6151 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2075 2093 2096 2099 2306 3347 1186 Size: 877 12 18 3 3 207 1041 4215 Fragments arranged by size: 4215 1041 877 207 18 12 3 3 BseYI C'CCAG_C Cuts at: 2158 3120 5579 6075 2158 Size: 962 2459 496 2459 Fragments arranged by size: 2459 2459 962 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2074 3389 1163 Size: 573 338 1315 4150 Fragments arranged by size: 4150 1315 573 338 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2322 3826 4587 5615 1540 Size: 275 186 321 1504 761 1028 2301 Fragments arranged by size: 2301 1504 1028 761 321 275 186 BsiHKAI G_wGCw'C Cuts at: 2316 3300 3519 4437 4522 5593 2316 Size: 984 219 918 85 1071 3099 Fragments arranged by size: 3099 1071 984 918 219 85 BsmI GAATG_Cn' Cuts at: 3508 3887 3980 3508 Size: 379 93 5904 Fragments arranged by size: 5904 379 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2192 2192 Size: 6376 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2232 2316 3300 3519 3695 4437 Size: 399 364 78 84 984 219 176 742 Cuts at: 4437 4522 5593 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 984 742 399 364 219 176 85 84 78 BspEI T'CCGG_A Cuts at: 6039 6039 Size: 6376 BspHI T'CATG_A Cuts at: 4265 5995 4265 Size: 1730 4646 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3731 844 Size: 2887 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2176 4263 1394 Size: 429 353 2087 3507 Fragments arranged by size: 3507 2087 429 353 BsrDI GCAATG_nn' Cuts at: 65 1248 4871 5045 65 Size: 1183 3623 174 1396 Fragments arranged by size: 3623 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5020 5207 1700 Size: 110 3210 187 2869 Fragments arranged by size: 3210 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3423 96 Size: 3327 3049 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6376 BssSI C'ACGA_G Cuts at: 4436 5448 4436 Size: 1012 5364 BstAPI GCAn_nnn'nTGC Cuts at: 794 3209 3286 794 Size: 2415 77 3884 Fragments arranged by size: 3884 2415 77 BstBI TT'CG_AA Cuts at: 5180 5180 Size: 6376 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6376 BstXI CCAn_nnnn'nTGG Cuts at: 2665 2665 Size: 6376 BstYI r'GATC_y Cuts at: 1972 2125 2565 3152 3493 3652 3706 4073 4458 Size: 153 440 587 341 159 54 367 385 Cuts at: 4458 4475 5927 6013 6025 6371 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 587 440 385 367 346 341 159 153 86 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3137 513 Size: 552 939 1133 3752 Fragments arranged by size: 3752 1133 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5637 Fragments arranged by size: 5637 683 56 BtsI GCAGTG_nn' Cuts at: 2062 2639 3892 4686 4706 2062 Size: 577 1253 794 20 3732 Fragments arranged by size: 3732 1253 794 577 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6376 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2402 2437 529 Size: 35 1838 35 4468 Fragments arranged by size: 4468 1838 35 35 DraI TTT'AAA Cuts at: 3680 3680 Size: 6376 DraIII CAC_nnn'GTG Cuts at: 2344 2344 Size: 6376 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5383 809 Size: 507 4067 1802 Fragments arranged by size: 4067 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2319 3823 4712 Size: 54 1425 50 275 18 489 1504 889 Cuts at: 4712 6341 8 Size: 1629 43 Fragments arranged by size: 1629 1504 1425 889 489 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2319 3823 1537 Size: 275 507 1504 4090 Fragments arranged by size: 4090 1504 507 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4306 1353 Size: 2953 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4963 5337 5483 387 Size: 1064 138 3374 374 146 1280 Fragments arranged by size: 3374 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2486 2659 3398 4439 5036 5823 6091 Size: 393 854 173 739 1041 597 787 268 Cuts at: 6091 1239 Size: 1524 Fragments arranged by size: 1524 1041 854 787 739 597 393 268 173 EcoNI CCTnn'n_nnAGG Cuts at: 3380 3380 Size: 6376 EcoO109I rG'GnC_Cy Cuts at: 2228 2228 Size: 6376 EcoRI G'AATT_C Cuts at: 3685 4063 3685 Size: 378 5998 EcoRV GAT'ATC Cuts at: 2716 2716 Size: 6376 FspI TGC'GCA Cuts at: 4882 4882 Size: 6376 HaeII r_GCGC'y Cuts at: 1678 2076 2223 2908 5523 1678 Size: 398 147 685 2615 2531 Fragments arranged by size: 2615 2531 685 398 147 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5017 5049 5091 5123 1373 Size: 32 135 32 3445 32 42 32 2626 Fragments arranged by size: 3445 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2365 3608 3720 3966 669 Size: 988 281 427 1243 112 246 3079 Fragments arranged by size: 3079 1243 988 427 281 246 112 HindIII A'AGCT_T Cuts at: 748 748 Size: 6376 HpaI GTT'AAC Cuts at: 3966 3966 Size: 6376 KasI G'GCGC_C Cuts at: 2072 2904 2072 Size: 832 5544 KpnI G_GTAC'C Cuts at: 3247 3701 3247 Size: 454 5922 MfeI C'AATT_G Cuts at: 2419 3007 3054 3975 2419 Size: 588 47 921 4820 Fragments arranged by size: 4820 921 588 47 MluI A'CGCG_T Cuts at: 6252 6252 Size: 6376 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5490 5674 1305 Size: 4185 184 2007 Fragments arranged by size: 4185 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4554 Fragments arranged by size: 4554 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2709 2738 3419 3512 3633 4334 4693 Size: 648 1543 29 681 93 121 701 359 Cuts at: 4693 4852 518 Size: 159 2042 Fragments arranged by size: 2042 1543 701 681 648 359 159 121 93 29 MspA1I CmG'CkG Cuts at: 1606 2070 3792 4469 5617 5862 1606 Size: 464 1722 677 1148 245 2120 Fragments arranged by size: 2120 1722 1148 677 464 245 NarI GG'CG_CC Cuts at: 2073 2905 2073 Size: 832 5544 NcoI C'CATG_G Cuts at: 513 1065 2004 3137 513 Size: 552 939 1133 3752 Fragments arranged by size: 3752 1133 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6376 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6376 NotI GC'GGCC_GC Cuts at: 3823 3823 Size: 6376 NspI r_CATG'y Cuts at: 1132 1229 3280 3355 3632 3734 5279 1132 Size: 97 2051 75 277 102 1545 2229 Fragments arranged by size: 2229 2051 1545 277 102 97 75 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6376 PciI A'CATG_T Cuts at: 3351 5275 3351 Size: 1924 4452 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6376 PfoI T'CCnGG_A Cuts at: 2355 3356 2355 Size: 1001 5375 PmeI GTTT'AAAC Cuts at: 3680 3680 Size: 6376 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3356 3388 4445 4477 5958 5990 3356 Size: 32 1057 32 1481 32 3742 Fragments arranged by size: 3742 1481 1057 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6376 PsiI TTA'TAA Cuts at: 3946 3946 Size: 6376 PspOMI G'GGCC_C Cuts at: 2228 2228 Size: 6376 PstI C_TGCA'G Cuts at: 830 3240 3561 3728 830 Size: 2410 321 167 3478 Fragments arranged by size: 3478 2410 321 167 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6376 SalI G'TCGA_C Cuts at: 1936 3606 3718 1936 Size: 1670 112 4594 Fragments arranged by size: 4594 1670 112 SbfI CC_TGCA'GG Cuts at: 3728 3728 Size: 6376 ScaI AGT'ACT Cuts at: 4624 6336 4624 Size: 1712 4664 SfcI C'TryA_G Cuts at: 826 1045 3236 3557 3724 4859 5540 5731 826 Size: 219 2191 321 167 1135 681 191 1471 Fragments arranged by size: 2191 1471 1135 681 321 219 191 167 SfoI GGC'GCC Cuts at: 2074 2906 2074 Size: 832 5544 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6376 SmaI CCC'GGG Cuts at: 1872 2155 3703 1872 Size: 283 1548 4545 Fragments arranged by size: 4545 1548 283 SmlI C'TyrA_G Cuts at: 820 1017 1943 4480 5381 5643 820 Size: 197 926 2537 901 262 1553 Fragments arranged by size: 2537 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4167 493 Size: 3674 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6376 SphI G_CATG'C Cuts at: 1132 3734 1132 Size: 2602 3774 SrfI GCCC'GGGC Cuts at: 2155 2155 Size: 6376 SspI AAT'ATT Cuts at: 5 52 2648 4300 5 Size: 47 2596 1652 2081 Fragments arranged by size: 2596 2081 1652 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6376 StyI C'CwwG_G Cuts at: 513 1065 2004 3137 3815 513 Size: 552 939 1133 678 3074 Fragments arranged by size: 3074 1133 939 678 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3112 4403 4420 4573 4758 5203 325 Size: 1255 1532 1291 17 153 185 445 1498 Fragments arranged by size: 1532 1498 1291 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3423 3577 3588 Size: 274 80 33 51 1443 1446 154 11 Cuts at: 3588 3663 4622 6334 96 Size: 75 959 1712 138 Fragments arranged by size: 1712 1446 1443 959 274 154 138 80 75 51 33 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2036 2068 3338 3370 6336 6368 2036 Size: 32 1270 32 2966 32 2044 Fragments arranged by size: 2966 2044 1270 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6376 XbaI T'CTAG_A Cuts at: 3496 3712 3496 Size: 216 6160 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6376 XmaI C'CCGG_G Cuts at: 1870 2153 3701 1870 Size: 283 1548 4545 Fragments arranged by size: 4545 1548 283 XmnI GAAnn'nnTTC Cuts at: 4505 4505 Size: 6376 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4184 276 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SwaI XcmI