(Linear) (Six-base) MAPSORT of: KIEE4140.seq Check: 525 from: 1 to: 1671 KIEE4140.orf With 182 enzymes: SgfI * March 22, 2012 17:27 .. AccI GT'mk_AC Cuts at: 0 1602 1671 Size: 1602 69 Acc65I G'GTAC_C Cuts at: 0 1238 1671 Size: 1238 433 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1393 1671 Size: 1393 278 AfeI AGC'GCT Cuts at: 0 216 1671 Size: 216 1455 AflIII A'CryG_T Cuts at: 0 1346 1671 Size: 1346 325 AleI CACnn'nnGTG Cuts at: 0 1507 1671 Size: 1507 164 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1128 1671 Size: 1128 543 AlwNI CAG_nnn'CTG Cuts at: 0 1281 1371 1592 1671 Size: 1281 90 221 79 Fragments arranged by size: 1281 221 90 79 ApaI G_GGCC'C Cuts at: 0 227 1671 Size: 227 1444 AseI AT'TA_AT Cuts at: 0 491 1671 Size: 491 1180 AvaI C'yCGr_G Cuts at: 0 148 1515 1671 Size: 148 1367 156 Fragments arranged by size: 1367 156 148 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1149 1182 1671 Size: 1149 33 489 Fragments arranged by size: 1149 489 33 BanI G'GyrC_C Cuts at: 0 67 899 1238 1671 Size: 67 832 339 433 Fragments arranged by size: 832 433 339 67 BanII G_rGCy'C Cuts at: 0 149 227 1671 Size: 149 78 1444 Fragments arranged by size: 1444 149 78 BbeI G_GCGC'C Cuts at: 0 71 903 1671 Size: 71 832 768 Fragments arranged by size: 832 768 71 BbsI GAAGACnn'nnnn_ Cuts at: 0 95 1422 1671 Size: 95 1327 249 Fragments arranged by size: 1327 249 95 BciVI GTATCCnnnnn_n' Cuts at: 0 358 1671 Size: 358 1313 BclI T'GATC_A Cuts at: 0 743 1671 Size: 743 928 BglII A'GATC_T Cuts at: 0 560 1488 1647 1671 Size: 560 928 159 24 Fragments arranged by size: 928 560 159 24 Bme1580I G_kGCm'C Cuts at: 0 227 1671 Size: 227 1444 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1530 1671 Size: 1530 141 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 481 654 1671 Size: 481 173 1017 Fragments arranged by size: 1017 481 173 Bpu10I CC'TnA_GC Cuts at: 0 403 1307 1671 Size: 403 904 364 Fragments arranged by size: 904 403 364 BsaHI Gr'CG_yC Cuts at: 0 68 158 900 1671 Size: 68 90 742 771 Fragments arranged by size: 771 742 90 68 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 58 70 88 91 94 301 1342 1671 Size: 58 12 18 3 3 207 1041 329 Fragments arranged by size: 1041 329 207 58 18 12 3 3 BseYI C'CCAG_C Cuts at: 0 153 1115 1671 Size: 153 962 556 Fragments arranged by size: 962 556 153 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 69 1384 1671 Size: 69 1315 287 Fragments arranged by size: 1315 287 69 BsiEI CG_ry'CG Cuts at: 0 317 1671 Size: 317 1354 BsiHKAI G_wGCw'C Cuts at: 0 311 1295 1514 1671 Size: 311 984 219 157 Fragments arranged by size: 984 311 219 157 BsmI GAATG_Cn' Cuts at: 0 1503 1671 Size: 1503 168 BsmBI CGTCTCn'nnnn_ Cuts at: 0 187 1671 Size: 187 1484 Bsp1286I G_dGCh'C Cuts at: 0 149 227 311 1295 1514 1671 Size: 149 78 84 984 219 157 Fragments arranged by size: 984 219 157 149 84 78 BsrBI CCG'CTC Cuts at: 0 171 1671 Size: 171 1500 BsrGI T'GTAC_A Cuts at: 0 1418 1671 Size: 1418 253 BstAPI GCAn_nnn'nTGC Cuts at: 0 1204 1281 1671 Size: 1204 77 390 Fragments arranged by size: 1204 390 77 BstXI CCAn_nnnn'nTGG Cuts at: 0 660 1671 Size: 660 1011 BstYI r'GATC_y Cuts at: 0 120 560 1147 1488 1647 1671 Size: 120 440 587 341 159 24 Fragments arranged by size: 587 440 341 159 120 24 BtgI C'CryG_G Cuts at: 0 1132 1671 Size: 1132 539 BtsI GCAGTG_nn' Cuts at: 0 57 634 1671 Size: 57 577 1037 Fragments arranged by size: 1037 577 57 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 397 432 1671 Size: 397 35 1239 Fragments arranged by size: 1239 397 35 DraIII CAC_nnn'GTG Cuts at: 0 339 1671 Size: 339 1332 EaeI y'GGCC_r Cuts at: 0 314 1671 Size: 314 1357 EagI C'GGCC_G Cuts at: 0 314 1671 Size: 314 1357 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 481 654 1393 1671 Size: 481 173 739 278 Fragments arranged by size: 739 481 278 173 EcoNI CCTnn'n_nnAGG Cuts at: 0 1375 1671 Size: 1375 296 EcoO109I rG'GnC_Cy Cuts at: 0 223 1671 Size: 223 1448 EcoRV GAT'ATC Cuts at: 0 711 1671 Size: 711 960 HaeII r_GCGC'y Cuts at: 0 71 218 903 1671 Size: 71 147 685 768 Fragments arranged by size: 768 685 147 71 HincII GTy'rAC Cuts at: 0 360 1603 1671 Size: 360 1243 68 Fragments arranged by size: 1243 360 68 KasI G'GCGC_C Cuts at: 0 67 899 1671 Size: 67 832 772 Fragments arranged by size: 832 772 67 KpnI G_GTAC'C Cuts at: 0 1242 1671 Size: 1242 429 MfeI C'AATT_G Cuts at: 0 414 1002 1049 1671 Size: 414 588 47 622 Fragments arranged by size: 622 588 414 47 MslI CAynn'nnrTG Cuts at: 0 704 733 1414 1507 1628 1671 Size: 704 29 681 93 121 43 Fragments arranged by size: 704 681 121 93 43 29 MspA1I CmG'CkG Cuts at: 0 65 1671 Size: 65 1606 NarI GG'CG_CC Cuts at: 0 68 900 1671 Size: 68 832 771 Fragments arranged by size: 832 771 68 NcoI C'CATG_G Cuts at: 0 1132 1671 Size: 1132 539 NspI r_CATG'y Cuts at: 0 1275 1350 1627 1671 Size: 1275 75 277 44 Fragments arranged by size: 1275 277 75 44 PciI A'CATG_T Cuts at: 0 1346 1671 Size: 1346 325 PfoI T'CCnGG_A Cuts at: 0 350 1351 1671 Size: 350 1001 320 Fragments arranged by size: 1001 350 320 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1351 1383 1671 Size: 1351 32 288 Fragments arranged by size: 1351 288 32 PspOMI G'GGCC_C Cuts at: 0 223 1671 Size: 223 1448 PstI C_TGCA'G Cuts at: 0 1235 1556 1671 Size: 1235 321 115 Fragments arranged by size: 1235 321 115 SalI G'TCGA_C Cuts at: 0 1601 1671 Size: 1601 70 SfcI C'TryA_G Cuts at: 0 1231 1552 1671 Size: 1231 321 119 Fragments arranged by size: 1231 321 119 SfoI GGC'GCC Cuts at: 0 69 901 1671 Size: 69 832 770 Fragments arranged by size: 832 770 69 SmaI CCC'GGG Cuts at: 0 150 1671 Size: 150 1521 SrfI GCCC'GGGC Cuts at: 0 150 1671 Size: 150 1521 SspI AAT'ATT Cuts at: 0 643 1671 Size: 643 1028 StyI C'CwwG_G Cuts at: 0 1132 1671 Size: 1132 539 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1107 1671 Size: 1107 564 TatI w'GTAC_w Cuts at: 0 1418 1572 1583 1658 1671 Size: 1418 154 11 75 13 Fragments arranged by size: 1418 154 75 13 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 31 63 1333 1365 1671 Size: 31 32 1270 32 306 Fragments arranged by size: 1270 306 32 32 31 XbaI T'CTAG_A Cuts at: 0 1491 1671 Size: 1491 180 XmaI C'CCGG_G Cuts at: 0 148 1671 Size: 148 1523 Enzymes that do cut: AccI Acc65I AcuI AfeI AflIII AleI AlfI AlwNI ApaI AseI AvaI BaeI BanI BanII BbeI BbsI BciVI BclI BglII Bme1580I BplI BpmI Bpu10I BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrGI BstAPI BstXI BstYI BtgI BtsI CspCI DraIII EaeI EagI Eco57MI EcoNI EcoO109I EcoRV HaeII HincII KasI KpnI MfeI MslI MspA1I NarI NcoI NspI PciI PfoI PpiI PspOMI PstI SalI SfcI SfoI SmaI SrfI SspI StyI TaqII TatI TstI XbaI XmaI Enzymes that do not cut: AarI AatII AclI AflII AgeI AhdI AloI ApaLI ApoI AscI AsiSI AvrII BamHI BbvCI BcgI BfrBI BglI BlpI BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BspEI BspHI BspMI BsrDI BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DrdI EarI EciI EcoICRI EcoRI FalI FseI FspI FspAI Hin4I HindIII HpaI MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI StuI SwaI Tth111I XcmI XhoI XmnI ZraI