Restriction map for
pFN21AE5070
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI Ksp632I MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tsp45I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 143 GGTACC G^GTACC
AcsI 216 GAATTC [GA]^AATT[CT]
AcyI 124 GGCGCC G[GA]^CG[CT]C
AfaI 144 GTAC GT^AC
AluI 208 AGCT AG^CT
AlwI 51 GATCC GGATC(4/5)
ApaI 31 GGGCCC GGGCC^C
ApoI 216 GAATTC [GA]^AATT[CT]
Asp718I 143 GGTACC G^GTACC
AspHI 207 GAGCTC G[AT]GC[AT]^C
BbeI 124 GGCGCC GGCGC^C
BbvCI 147 CCTCAGC CCTCAGC(-5/-2)
BbvI 164 GCAGC GCAGC(8/12)
BfaI 260 CTAG C^TAG
BsaHI 124 GGCGCC G[GA]^CG[CT]C
BsgI 46 GTGCAG GTGCAG(16/14)
BsiHKAI 207 GAGCTC G[AT]GC[AT]^C
BsmAI 115 GAGAC GTCTC(1/5)
BsmFI 131 GGGAC GGGAC(10/14)
BsrI 6 CCAGT ACTGG(1/-1)
BstF5I 63 CATCC GGATG(2/0)
BstNI 66 CCAGG CC^[AT]GG
BstYI 50 AGATCC [GA]^GATC[CT]
DdeI 148 CTCAG C^T[ACGT]AG
DpnII 51 GATC ^GATC
DraII 131 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 189 CCGCGG C^C[GA][CT]GG
EcoO109I 131 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 216 GAATTC G^AATTC
EcoRII 66 CCAGG ^CC[AT]GG
FokI 63 CATCC GGATG(9/13)
HaeII 124 GGCGCC [GA]GCGC^[CT]
HgaI 56 GCGTC GACGC(5/10)
Hin1I 124 GGCGCC G[GA]^CG[CT]C
HinfI 117 GACTC G^A[ACGT]TC
HphI 213 GGTGA GGTGA(8/7)
KasI 124 GGCGCC G^GCGCC
KpnI 143 GGTACC GGTAC^C
KspI 189 CCGCGG CCGC^GG
MaeI 260 CTAG C^TAG
MaeII 80 ACGT A^CGT
MboI 51 GATC ^GATC
MboII 250 TCTTC GAAGA(8/7)
MflI 50 AGATCC [GA]^GATC[CT]
MspA1I 189 CCGCGG C[AC]G^C[GT]G
MvaI 66 CCAGG CC^[AT]GG
NarI 124 GGCGCC GG^CGCC
NdeII 51 GATC ^GATC
NlaIII 228 CATG CATG^
PleI 117 GACTC GAGTC(4/5)
PpuMI 131 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 144 GTAC GT^AC
SacI 207 GAGCTC GAGCT^C
SacII 189 CCGCGG CCGC^GG
Sau3AI 51 GATC ^GATC
SfoI 124 GGCGCC GGC^GCC
SmaI 28 CCCGGG CCC^GGG
TseI 164 GCAGC G^C[AT]GC
Tsp509I 217 AATT ^AATT
XhoII 50 AGATCC [GA]^GATC[CT]
XmaCI 28 CCCGGG C^CCGGG
XmaI 28 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AvaI 28 CCCGGG C^[CT]CG[GA]G
119 CTCGGG
AvaII 132 GGACC G^G[AT]CC
224 GGACC
BanI 124 GGCGCC G^G[CT][GA]CC
143 GGTACC
BanII 31 GGGCCC G[GA]GC[CT]^C
207 GAGCTC
BsoBI 28 CCCGGG C^[CT]CG[GA]G
119 CTCGGG
CfoI 125 GCGC GCG^C
167 GCGC
FauI 14 GCGGG CCCGC(4/6)
191 GCGGG
Fnu4HI 164 GCAGC GC^[ACGT]GC
188 GCCGC
HhaI 125 GCGC GCG^C
167 GCGC
HinP1I 125 GCGC G^CGC
167 GCGC
ItaI 164 GCAGC GC^[ACGT]GC
188 GCCGC
MwoI 158 GCTGGGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
164 GCAGCGCGAGC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AccII AciI BcnI BmyI BsaJI
BseRI BsiYI BslI Bsp1286I BssKI
BstUI Cac8I Cfr13I HaeIII HapII
HpaII MnlI MspI MvnI NciI
NlaIV Sau96I ScrFI