(Linear) (Six-base) MAPSORT of: KIEE5070.seq Check: 4573 from: 1 to: 279 KIEE5070.orf With 182 enzymes: SgfI * December 5, 2014 12:59 .. Acc65I G'GTAC_C Cuts at: 0 143 279 Size: 143 136 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 236 279 Size: 236 43 ApaI G_GGCC'C Cuts at: 0 35 279 Size: 35 244 ApoI r'AATT_y Cuts at: 0 216 279 Size: 216 63 AvaI C'yCGr_G Cuts at: 0 28 119 279 Size: 28 91 160 Fragments arranged by size: 160 91 28 BanI G'GyrC_C Cuts at: 0 124 143 279 Size: 124 19 136 Fragments arranged by size: 136 124 19 BanII G_rGCy'C Cuts at: 0 35 211 279 Size: 35 176 68 Fragments arranged by size: 176 68 35 BbeI G_GCGC'C Cuts at: 0 128 279 Size: 128 151 BbvCI CC'TCA_GC Cuts at: 0 148 279 Size: 148 131 Bme1580I G_kGCm'C Cuts at: 0 35 197 279 Size: 35 162 82 Fragments arranged by size: 162 82 35 BmgBI CAC'GTC Cuts at: 0 81 279 Size: 81 198 Bpu10I CC'TnA_GC Cuts at: 0 148 279 Size: 148 131 BsaHI Gr'CG_yC Cuts at: 0 125 279 Size: 125 154 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 115 118 190 193 279 Size: 115 3 72 3 86 Fragments arranged by size: 115 86 72 3 3 BseYI C'CCAG_C Cuts at: 0 158 271 279 Size: 158 113 8 Fragments arranged by size: 158 113 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 279 Size: 67 212 BsiHKAI G_wGCw'C Cuts at: 0 211 279 Size: 211 68 Bsp1286I G_dGCh'C Cuts at: 0 35 197 211 279 Size: 35 162 14 68 Fragments arranged by size: 162 68 35 14 BstYI r'GATC_y Cuts at: 0 50 279 Size: 50 229 BtgI C'CryG_G Cuts at: 0 189 279 Size: 189 90 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 127 279 Size: 127 152 EcoICRI GAG'CTC Cuts at: 0 209 279 Size: 209 70 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 236 279 Size: 236 43 EcoO109I rG'GnC_Cy Cuts at: 0 132 279 Size: 132 147 EcoRI G'AATT_C Cuts at: 0 216 279 Size: 216 63 HaeII r_GCGC'y Cuts at: 0 128 279 Size: 128 151 KasI G'GCGC_C Cuts at: 0 124 279 Size: 124 155 KpnI G_GTAC'C Cuts at: 0 147 279 Size: 147 132 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 71 279 Size: 71 208 MspA1I CmG'CkG Cuts at: 0 191 279 Size: 191 88 NarI GG'CG_CC Cuts at: 0 125 279 Size: 125 154 PfoI T'CCnGG_A Cuts at: 0 65 279 Size: 65 214 PpuMI rG'GwC_Cy Cuts at: 0 132 279 Size: 132 147 PspOMI G'GGCC_C Cuts at: 0 31 279 Size: 31 248 SacI G_AGCT'C Cuts at: 0 211 279 Size: 211 68 SacII CC_GC'GG Cuts at: 0 192 279 Size: 192 87 SanDI GG'GwC_CC Cuts at: 0 132 279 Size: 132 147 SfoI GGC'GCC Cuts at: 0 126 279 Size: 126 153 SmaI CCC'GGG Cuts at: 0 30 279 Size: 30 249 SrfI GCCC'GGGC Cuts at: 0 30 279 Size: 30 249 XmaI C'CCGG_G Cuts at: 0 28 279 Size: 28 251 Enzymes that do cut: Acc65I AcuI ApaI ApoI AvaI BanI BanII BbeI BbvCI Bme1580I BmgBI Bpu10I BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BstYI BtgI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII KasI KpnI MmeI MspA1I NarI PfoI PpuMI PspOMI SacI SacII SanDI SfoI SmaI SrfI XmaI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EcoNI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI MscI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI