################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AflII AflIII AgeI AhdI Aor51HI AscI Asp718I AspEI AspI AviII AvrII BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FauI FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MfeI MluI MroI MunI MvnI NciI NcoI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PstI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AclI 2330 AACGTT AA^CGTT Alw44I 706 GTGCAC G^TGCAC AlwNI 1235 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 2285 GGGCCC GGGCC^C ApaLI 706 GTGCAC G^TGCAC AseI 2244 ATTAAT AT^TAAT AsnI 2244 ATTAAT AT^TAAT AspHI 706 GTGCAC G[AT]GC[AT]^C AvaI 398 CTCGAG C^[CT]CG[GA]G BalI 2384 TGGCCA TGG^CCA BanII 2285 GGGCCC G[GA]GC[CT]^C BbeI 2098 GGCGCC GGCGC^C BglII 2525 AGATCT A^GATCT BsaBI 1290 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 1972 GTGCAG GTGCAG(16/14) BsiHKAI 706 GTGCAC G[AT]GC[AT]^C BsmI 1363 GAATGC GAATGC(1/-1) BsoBI 398 CTCGAG C^[CT]CG[GA]G BspHI 1911 TCATGA T^CATGA BspMI 1822 GCAGGT ACCTGC(4/8) BsrBI 112 GAGCGG CCGCTC(-3/-3) BsrDI 10 GCAATG GCAATG(2/0) BstAPI 2586 GCATCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2341 GGTCACC G^GT[ACGT]ACC BstPI 2341 GGTCACC G^GT[ACGT]ACC CfoI 2099 GCGC GCG^C EcoNI 946 CCTAAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2341 GGTCACC G^GT[ACGT]ACC EcoRI 851 GAATTC G^AATTC HaeII 2098 GGCGCC [GA]GCGC^[CT] HgaI 1251 GACGC GACGC(5/10) HhaI 2099 GCGC GCG^C HinP1I 2099 GCGC G^CGC KasI 2098 GGCGCC G^GCGCC MamI 1290 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2384 TGGCCA TGG^CCA MscI 2384 TGGCCA TGG^CCA NaeI 1520 GCCGGC GCC^GGC NarI 2098 GGCGCC GG^CGCC NdeI 2513 CATATG CA^TATG NgoMIV 1520 GCCGGC G^CCGGC NheI 717 GCTAGC G^CTAGC PaeR7I 398 CTCGAG C^TCGAG PpuMI 1824 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 2244 ATTAAT AT^TAAT Psp1406I 2330 AACGTT AA^CGTT RcaI 1911 TCATGA T^CATGA ScaI 1870 AGTACT AGT^ACT SfoI 2098 GGCGCC GGC^GCC SmlI 398 CTCGAG C^T[CT][GA]AG SpeI 577 ACTAGT A^CTAGT SspI 67 AATATT AAT^ATT XhoI 398 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 1251 GACGCC G[GA]^CG[CT]C 2098 GGCGCC BamHI 600 GGATCC G^GATCC 2068 GGATCC BciVI 2224 GGATAC GTATCC(6/5) 2320 GGATAC BmyI 706 GTGCAC G[AGT]GC[ACT]^C 2285 GGGCCC BsaHI 1251 GACGCC G[GA]^CG[CT]C 2098 GGCGCC Bsp1286I 706 GTGCAC G[AGT]GC[ACT]^C 2285 GGGCCC BsrFI 496 GCCGGT [GA]^CCGG[CT] 1520 GCCGGC Cfr10I 496 GCCGGT [GA]^CCGG[CT] 1520 GCCGGC DsaI 1656 CCGTGG C^C[GA][CT]GG 2205 CCACGG EaeI 1518 TGGCCG [CT]^GGCC[GA] 2384 TGGCCA EcoRV 663 GATATC GAT^ATC 2260 GATATC EcoT14I 468 CCAAGG C^C[AT][AT]GG 2613 CCTTGG HapII 497 CCGG C^CGG 1521 CCGG Hin1I 1251 GACGCC G[GA]^CG[CT]C 2098 GGCGCC HpaII 497 CCGG C^CGG 1521 CCGG MspA1I 25 CAGCTG C[AC]G^C[GT]G 746 CAGCTG MspI 497 CCGG C^CGG 1521 CCGG PvuII 25 CAGCTG CAG^CTG 746 CAGCTG SfcI 1156 CTACAG C^T[GA][CT]AG 1918 CTACAG SnaBI 1609 TACGTA TAC^GTA 1621 TACGTA StyI 468 CCAAGG C^C[AT][AT]GG 2613 CCTTGG TaqI 399 TCGA T^CGA 2169 TCGA TthHB8I 399 TCGA T^CGA 2169 TCGA XcmI 587 CCAACATACGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2290 CCAGTCACTCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AocI ApoI Asp700I AvaII BanI BbvI BfaI BpmI BsaAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI DraII DraIII EarI Eco81I EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI