(Circular) (Six-base) MAPSORT of: pFN21AE6703.seq Check: 1291 from: 1 to: 7351 pFN21AE6703 7351 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5161 278 Size: 53 83 186 936 3625 2468 Fragments arranged by size: 3625 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4694 1937 Size: 2757 4594 Acc65I G'GTAC_C Cuts at: 4672 4672 Size: 7351 AclI AA'CG_TT Cuts at: 1563 4336 5478 5851 1563 Size: 2773 1142 373 3063 Fragments arranged by size: 3063 2773 1142 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3033 3792 4255 5414 6798 7066 1632 Size: 1401 759 463 1159 1384 268 1917 Fragments arranged by size: 1917 1401 1384 1159 759 463 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7154 AflIII A'CryG_T Cuts at: 1203 6250 7227 1203 Size: 5047 977 1327 Fragments arranged by size: 5047 1327 977 AgeI A'CCGG_T Cuts at: 6182 6182 Size: 7351 AhdI GACnn_n'nnGTC Cuts at: 6080 6080 Size: 7351 AleI CACnn'nnGTG Cuts at: 1166 2708 1166 Size: 1542 5809 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7351 AlwNI CAG_nnn'CTG Cuts at: 3245 5094 6666 3245 Size: 1849 1572 3930 Fragments arranged by size: 3930 1849 1572 ApaI G_GGCC'C Cuts at: 4294 4294 Size: 7351 ApaLI G'TGCA_C Cuts at: 2711 5408 6564 2711 Size: 2697 1156 3498 Fragments arranged by size: 3498 2697 1156 ApoI r'AATT_y Cuts at: 1493 2307 2511 2783 2847 2856 3800 3858 4094 Size: 814 204 272 64 9 944 58 236 Cuts at: 4094 4660 4886 5038 7147 1493 Size: 566 226 152 2109 1697 Fragments arranged by size: 2109 1697 944 814 566 272 236 226 204 152 64 58 9 AseI AT'TA_AT Cuts at: 160 1032 4250 5905 160 Size: 872 3218 1655 1606 Fragments arranged by size: 3218 1655 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7351 AvaI C'yCGr_G Cuts at: 1870 1943 2403 4676 1870 Size: 73 460 2273 4545 Fragments arranged by size: 4545 2273 460 73 BamHI G'GATC_C Cuts at: 2605 4073 4681 5048 2605 Size: 1468 608 367 4908 Fragments arranged by size: 4908 1468 608 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2191 3581 4103 4672 6127 Size: 325 217 627 404 1390 522 569 1455 Cuts at: 6127 618 Size: 1842 Fragments arranged by size: 1842 1455 1390 627 569 522 404 325 217 BanII G_rGCy'C Cuts at: 1391 4294 4670 1391 Size: 2903 376 4072 Fragments arranged by size: 4072 2903 376 BbeI G_GCGC'C Cuts at: 4107 4107 Size: 7351 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6380 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5542 5576 1903 Size: 34 3605 34 3678 Fragments arranged by size: 3678 3605 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4223 4319 5243 6453 4223 Size: 96 924 1210 5121 Fragments arranged by size: 5121 1210 924 96 BclI T'GATC_A Cuts at: 1548 1548 Size: 7351 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5962 136 Size: 107 122 71 631 605 4290 1525 Fragments arranged by size: 4290 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4530 7346 4530 Size: 2816 4535 BlpI GC'TnA_GC Cuts at: 4768 4768 Size: 7351 Bme1580I G_kGCm'C Cuts at: 1790 2715 4294 5412 6568 1790 Size: 925 1579 1118 1156 2573 Fragments arranged by size: 2573 1579 1156 1118 925 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7351 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2829 3191 4289 Size: 416 53 83 399 24 1412 362 1098 Cuts at: 4289 5082 6040 442 Size: 793 958 1753 Fragments arranged by size: 1753 1412 1098 958 793 416 399 362 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2726 1056 Size: 1670 5681 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4250 4250 Size: 7351 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2149 2775 3657 3837 6011 1239 Size: 910 626 882 180 2174 2579 Fragments arranged by size: 2579 2174 910 882 626 180 Bpu10I CC'TnA_GC Cuts at: 2187 2187 Size: 7351 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5476 6341 6639 5476 Size: 865 298 6188 Fragments arranged by size: 6188 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6014 882 Size: 617 4515 2219 Fragments arranged by size: 4515 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2521 3616 3628 4628 5142 493 Size: 711 1317 1095 12 1000 514 2702 Fragments arranged by size: 2702 1317 1095 1000 711 514 12 BsaBI GATnn'nnATC Cuts at: 3299 7345 3299 Size: 4046 3305 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3257 4104 5158 5540 Size: 53 83 186 936 1724 847 1054 382 Cuts at: 5540 6175 275 Size: 635 1451 Fragments arranged by size: 1724 1451 1054 936 847 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5784 6182 6456 6603 7014 5784 Size: 398 274 147 411 6121 Fragments arranged by size: 6121 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7126 Fragments arranged by size: 7126 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 3500 3840 1186 Size: 895 1419 340 4697 Fragments arranged by size: 4697 1419 895 340 BseYI C'CCAG_C Cuts at: 2032 4286 6554 7050 2032 Size: 2254 2268 496 2333 Fragments arranged by size: 2333 2268 2254 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3998 1163 Size: 573 2262 4516 Fragments arranged by size: 4516 2262 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4801 5562 6590 1540 Size: 275 186 2800 761 1028 2301 Fragments arranged by size: 2800 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2715 5412 5497 6568 2715 Size: 2697 85 1071 3498 Fragments arranged by size: 3498 2697 1071 85 BsmI GAATG_Cn' Cuts at: 3374 4862 4955 3374 Size: 1488 93 5770 Fragments arranged by size: 5770 1488 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2715 4294 4670 5412 5497 6568 1391 Size: 399 925 1579 376 742 85 1071 2174 Fragments arranged by size: 2174 1579 1071 925 742 399 376 85 BspEI T'CCGG_A Cuts at: 7014 7014 Size: 7351 BspHI T'CATG_A Cuts at: 3916 5240 6970 3916 Size: 1324 1730 4297 Fragments arranged by size: 4297 1730 1324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3818 4706 844 Size: 2974 888 3489 Fragments arranged by size: 3489 2974 888 BsrBI CCG'CTC Cuts at: 1394 1823 2119 5238 1394 Size: 429 296 3119 3507 Fragments arranged by size: 3507 3119 429 296 BsrDI GCAATG_nn' Cuts at: 65 1248 2022 5846 6020 65 Size: 1183 774 3824 174 1396 Fragments arranged by size: 3824 1396 1183 774 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2501 3525 5995 6182 1700 Size: 110 691 1024 2470 187 2869 Fragments arranged by size: 2869 2470 1024 691 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7351 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7351 BssSI C'ACGA_G Cuts at: 5411 6423 5411 Size: 1012 6339 BstAPI GCAn_nnn'nTGC Cuts at: 794 4597 794 Size: 3803 3548 BstBI TT'CG_AA Cuts at: 6155 6155 Size: 7351 BstEII G'GTnAC_C Cuts at: 1184 4346 1184 Size: 3162 4189 BstXI CCAn_nnnn'nTGG Cuts at: 2480 2575 3817 2480 Size: 95 1242 6014 Fragments arranged by size: 6014 1242 95 BstYI r'GATC_y Cuts at: 1972 2365 2605 3533 4073 4308 4382 4530 4681 Size: 393 240 928 540 235 74 148 151 Cuts at: 4681 5048 5433 5450 6902 6988 7000 7346 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 928 540 393 385 367 346 240 235 151 148 86 74 17 12 Bsu36I CC'TnA_GG Cuts at: 3111 3972 4539 3111 Size: 861 567 5923 Fragments arranged by size: 5923 861 567 BtgI C'CryG_G Cuts at: 513 1065 2004 3661 4210 513 Size: 552 939 1657 549 3654 Fragments arranged by size: 3654 1657 939 552 549 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6612 Fragments arranged by size: 6612 683 56 BtsI GCAGTG_nn' Cuts at: 4867 5661 5681 4867 Size: 794 20 6537 Fragments arranged by size: 6537 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7351 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7316 DraI TTT'AAA Cuts at: 3414 4000 4111 4655 3414 Size: 586 111 544 6110 Fragments arranged by size: 6110 586 544 111 DraIII CAC_nnn'GTG Cuts at: 3743 3745 4305 3743 Size: 2 560 6789 Fragments arranged by size: 6789 560 2 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6358 809 Size: 507 5042 1802 Fragments arranged by size: 5042 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3523 4389 4798 Size: 54 1425 50 275 18 1693 866 409 Cuts at: 4798 5687 7316 8 Size: 889 1629 43 Fragments arranged by size: 1693 1629 1425 889 866 409 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4798 1537 Size: 275 2986 4090 Fragments arranged by size: 4090 2986 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2210 3333 3840 5281 1353 Size: 857 1123 507 1441 3423 Fragments arranged by size: 3423 1441 1123 857 507 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5938 6312 6458 387 Size: 1064 138 4349 374 146 1280 Fragments arranged by size: 4349 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2149 2775 3033 3657 3792 3837 4255 Size: 393 517 626 258 624 135 45 418 Cuts at: 4255 5414 6011 6798 7066 1239 Size: 1159 597 787 268 1524 Fragments arranged by size: 1524 1159 787 626 624 597 517 418 393 268 258 135 45 EcoNI CCTnn'n_nnAGG Cuts at: 2955 2955 Size: 7351 EcoO109I rG'GnC_Cy Cuts at: 3830 4290 4291 3830 Size: 460 1 6890 Fragments arranged by size: 6890 460 1 EcoRI G'AATT_C Cuts at: 2856 4660 5038 2856 Size: 1804 378 5169 Fragments arranged by size: 5169 1804 378 EcoRV GAT'ATC Cuts at: 2670 4267 2670 Size: 1597 5754 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4634 4634 Size: 7351 FspI TGC'GCA Cuts at: 5857 5857 Size: 7351 HaeII r_GCGC'y Cuts at: 1678 4107 6498 1678 Size: 2429 2391 2531 Fragments arranged by size: 2531 2429 2391 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2209 2241 2319 2351 2732 Size: 32 135 32 637 32 78 32 381 Cuts at: 2732 2764 3525 3557 4522 4554 5992 6024 6066 Size: 32 761 32 965 32 1438 32 42 Cuts at: 6066 6098 1373 Size: 32 2626 Fragments arranged by size: 2626 1438 965 761 637 381 135 78 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4695 4941 669 Size: 988 281 2757 246 3079 Fragments arranged by size: 3079 2757 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7351 HpaI GTT'AAC Cuts at: 4941 4941 Size: 7351 KasI G'GCGC_C Cuts at: 4103 4103 Size: 7351 KpnI G_GTAC'C Cuts at: 4676 4676 Size: 7351 MfeI C'AATT_G Cuts at: 4950 4950 Size: 7351 MluI A'CGCG_T Cuts at: 7227 7227 Size: 7351 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2428 2898 6465 6649 1305 Size: 1123 470 3567 184 2007 Fragments arranged by size: 3567 2007 1123 470 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4391 10 Size: 54 1425 343 2559 2970 Fragments arranged by size: 2970 2559 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 2600 2708 5309 5668 5827 518 Size: 648 1044 390 108 2601 359 159 2042 Fragments arranged by size: 2601 2042 1044 648 390 359 159 108 MspA1I CmG'CkG Cuts at: 1606 2032 2753 4767 5444 6592 6837 1606 Size: 426 721 2014 677 1148 245 2120 Fragments arranged by size: 2120 2014 1148 721 677 426 245 NaeI GCC'GGC Cuts at: 3527 3527 Size: 7351 NarI GG'CG_CC Cuts at: 4104 4104 Size: 7351 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5860 Fragments arranged by size: 5860 939 552 NdeI CA'TA_TG Cuts at: 387 4519 387 Size: 4132 3219 NgoMIV G'CCGG_C Cuts at: 3525 3525 Size: 7351 NheI G'CTAG_C Cuts at: 1052 2722 1052 Size: 1670 5681 NotI GC'GGCC_GC Cuts at: 4798 4798 Size: 7351 NspI r_CATG'y Cuts at: 1132 1229 4709 6254 1132 Size: 97 3480 1545 2229 Fragments arranged by size: 3480 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7351 PciI A'CATG_T Cuts at: 6250 6250 Size: 7351 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7351 PfoI T'CCnGG_A Cuts at: 2126 3634 4449 2126 Size: 1508 815 5028 Fragments arranged by size: 5028 1508 815 PmeI GTTT'AAAC Cuts at: 4655 4655 Size: 7351 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5420 5452 6933 6965 5420 Size: 32 1481 32 5806 Fragments arranged by size: 5806 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3830 3830 Size: 7351 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7351 PsiI TTA'TAA Cuts at: 3710 4921 3710 Size: 1211 6140 PspOMI G'GGCC_C Cuts at: 4290 4290 Size: 7351 PstI C_TGCA'G Cuts at: 830 4703 830 Size: 3873 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7351 PvuII CAG'CTG Cuts at: 2032 2753 2032 Size: 721 6630 SalI G'TCGA_C Cuts at: 1936 4693 1936 Size: 2757 4594 SbfI CC_TGCA'GG Cuts at: 4703 4703 Size: 7351 ScaI AGT'ACT Cuts at: 3877 5599 7311 3877 Size: 1722 1712 3917 Fragments arranged by size: 3917 1722 1712 SfcI C'TryA_G Cuts at: 826 1045 3161 3923 4699 5834 6515 6706 826 Size: 219 2116 762 776 1135 681 191 1471 Fragments arranged by size: 2116 1471 1135 776 762 681 219 191 SfoI GGC'GCC Cuts at: 4105 4105 Size: 7351 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7351 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7351 SmaI CCC'GGG Cuts at: 1872 4678 1872 Size: 2806 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2403 5455 6356 6618 820 Size: 197 926 460 3052 901 262 1553 Fragments arranged by size: 3052 1553 926 901 460 262 197 SnaBI TAC'GTA Cuts at: 493 3616 3628 5142 493 Size: 3123 12 1514 2702 Fragments arranged by size: 3123 2702 1514 12 SpeI A'CTAG_T Cuts at: 152 2582 152 Size: 2430 4921 SphI G_CATG'C Cuts at: 1132 4709 1132 Size: 3577 3774 SspI AAT'ATT Cuts at: 5 52 2074 5275 5 Size: 47 2022 3201 2081 Fragments arranged by size: 3201 2081 2022 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7351 StyI C'CwwG_G Cuts at: 513 1065 2004 2473 4618 4790 513 Size: 552 939 469 2145 172 3074 Fragments arranged by size: 3074 2145 939 552 469 172 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2024 5378 5395 5548 5733 6178 325 Size: 1255 444 3354 17 153 185 445 1498 Fragments arranged by size: 3354 1498 1255 445 444 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3875 5597 7309 Size: 274 80 33 51 1443 1898 1722 1712 Cuts at: 7309 96 Size: 138 Fragments arranged by size: 1898 1722 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3731 3763 4202 4234 7311 7343 3731 Size: 32 439 32 3077 32 3739 Fragments arranged by size: 3739 3077 439 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7351 XbaI T'CTAG_A Cuts at: 4687 4687 Size: 7351 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2599 4302 2599 Size: 1703 5648 XhoI C'TCGA_G Cuts at: 1943 2403 1943 Size: 460 6891 XmaI C'CCGG_G Cuts at: 1870 4676 1870 Size: 2806 4545 XmnI GAAnn'nnTTC Cuts at: 2398 2455 2895 3048 5480 2398 Size: 57 440 153 2432 4269 Fragments arranged by size: 4269 2432 440 153 57 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5159 276 Size: 53 83 186 936 3625 2468 Fragments arranged by size: 3625 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoICRI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI