(Linear) (Six-base) MAPSORT of: KIEE6703.seq Check: 7308 from: 1 to: 2646 KIEE6703.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AclI AA'CG_TT Cuts at: 0 2331 2646 Size: 2331 315 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1028 1787 2250 2646 Size: 1028 759 463 396 Fragments arranged by size: 1028 759 463 396 AleI CACnn'nnGTG Cuts at: 0 703 2646 Size: 703 1943 AlwNI CAG_nnn'CTG Cuts at: 0 1240 2646 Size: 1240 1406 ApaI G_GGCC'C Cuts at: 0 2289 2646 Size: 2289 357 ApaLI G'TGCA_C Cuts at: 0 706 2646 Size: 706 1940 ApoI r'AATT_y Cuts at: 0 302 506 778 842 851 1795 1853 2089 Size: 302 204 272 64 9 944 58 236 Cuts at: 2089 2646 Size: 557 Fragments arranged by size: 944 557 302 272 236 204 64 58 9 AseI AT'TA_AT Cuts at: 0 2245 2646 Size: 2245 401 AvaI C'yCGr_G Cuts at: 0 398 2646 Size: 398 2248 BamHI G'GATC_C Cuts at: 0 600 2068 2646 Size: 600 1468 578 Fragments arranged by size: 1468 600 578 BanI G'GyrC_C Cuts at: 0 186 1576 2098 2646 Size: 186 1390 522 548 Fragments arranged by size: 1390 548 522 186 BanII G_rGCy'C Cuts at: 0 2289 2646 Size: 2289 357 BbeI G_GCGC'C Cuts at: 0 2102 2646 Size: 2102 544 BciVI GTATCCnnnnn_n' Cuts at: 0 2218 2314 2646 Size: 2218 96 332 Fragments arranged by size: 2218 332 96 BglII A'GATC_T Cuts at: 0 2525 2646 Size: 2525 121 Bme1580I G_kGCm'C Cuts at: 0 710 2289 2646 Size: 710 1579 357 Fragments arranged by size: 1579 710 357 BmrI ACTGGGnnnn_n' Cuts at: 0 824 1186 2284 2646 Size: 824 362 1098 362 Fragments arranged by size: 1098 824 362 362 BmtI G_CTAG'C Cuts at: 0 721 2646 Size: 721 1925 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2245 2646 Size: 2245 401 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 144 770 1652 1832 2646 Size: 144 626 882 180 814 Fragments arranged by size: 882 814 626 180 144 Bpu10I CC'TnA_GC Cuts at: 0 182 2646 Size: 182 2464 BsaAI yAC'GTr Cuts at: 0 516 1611 1623 2623 2646 Size: 516 1095 12 1000 23 Fragments arranged by size: 1095 1000 516 23 12 BsaBI GATnn'nnATC Cuts at: 0 1294 2646 Size: 1294 1352 BsaHI Gr'CG_yC Cuts at: 0 1252 2099 2646 Size: 1252 847 547 Fragments arranged by size: 1252 847 547 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 76 1495 1835 2646 Size: 76 1419 340 811 Fragments arranged by size: 1419 811 340 76 BseYI C'CCAG_C Cuts at: 0 27 2281 2646 Size: 27 2254 365 Fragments arranged by size: 2254 365 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1993 2646 Size: 1993 653 BsiHKAI G_wGCw'C Cuts at: 0 710 2646 Size: 710 1936 BsmI GAATG_Cn' Cuts at: 0 1369 2646 Size: 1369 1277 Bsp1286I G_dGCh'C Cuts at: 0 710 2289 2646 Size: 710 1579 357 Fragments arranged by size: 1579 710 357 BspHI T'CATG_A Cuts at: 0 1911 2646 Size: 1911 735 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1813 2646 Size: 1813 833 BsrBI CCG'CTC Cuts at: 0 114 2646 Size: 114 2532 BsrDI GCAATG_nn' Cuts at: 0 17 2646 Size: 17 2629 BsrFI r'CCGG_y Cuts at: 0 496 1520 2646 Size: 496 1024 1126 Fragments arranged by size: 1126 1024 496 BstAPI GCAn_nnn'nTGC Cuts at: 0 2592 2646 Size: 2592 54 BstEII G'GTnAC_C Cuts at: 0 2341 2646 Size: 2341 305 BstXI CCAn_nnnn'nTGG Cuts at: 0 475 570 1812 2646 Size: 475 95 1242 834 Fragments arranged by size: 1242 834 475 95 BstYI r'GATC_y Cuts at: 0 360 600 1528 2068 2303 2377 2525 2646 Size: 360 240 928 540 235 74 148 121 Fragments arranged by size: 928 540 360 240 235 148 121 74 Bsu36I CC'TnA_GG Cuts at: 0 1106 1967 2534 2646 Size: 1106 861 567 112 Fragments arranged by size: 1106 861 567 112 BtgI C'CryG_G Cuts at: 0 1656 2205 2646 Size: 1656 549 441 Fragments arranged by size: 1656 549 441 DraI TTT'AAA Cuts at: 0 1409 1995 2106 2646 Size: 1409 586 111 540 Fragments arranged by size: 1409 586 540 111 DraIII CAC_nnn'GTG Cuts at: 0 1738 1740 2300 2646 Size: 1738 2 560 346 Fragments arranged by size: 1738 560 346 2 EaeI y'GGCC_r Cuts at: 0 1518 2384 2646 Size: 1518 866 262 Fragments arranged by size: 1518 866 262 EarI CTCTTCn'nnn_ Cuts at: 0 205 1328 1835 2646 Size: 205 1123 507 811 Fragments arranged by size: 1123 811 507 205 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 144 770 1028 1652 1787 1832 2250 2646 Size: 144 626 258 624 135 45 418 396 Fragments arranged by size: 626 624 418 396 258 144 135 45 EcoNI CCTnn'n_nnAGG Cuts at: 0 950 2646 Size: 950 1696 EcoO109I rG'GnC_Cy Cuts at: 0 1825 2285 2286 2646 Size: 1825 460 1 360 Fragments arranged by size: 1825 460 360 1 EcoRI G'AATT_C Cuts at: 0 851 2646 Size: 851 1795 EcoRV GAT'ATC Cuts at: 0 665 2262 2646 Size: 665 1597 384 Fragments arranged by size: 1597 665 384 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2629 2646 Size: 2629 17 HaeII r_GCGC'y Cuts at: 0 2102 2646 Size: 2102 544 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 204 236 314 346 727 759 1520 1552 Size: 204 32 78 32 381 32 761 32 Cuts at: 1552 2517 2549 2646 Size: 965 32 97 Fragments arranged by size: 965 761 381 204 97 78 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 2098 2646 Size: 2098 548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 423 893 2646 Size: 423 470 1753 Fragments arranged by size: 1753 470 423 MscI TGG'CCA Cuts at: 0 2386 2646 Size: 2386 260 MslI CAynn'nnrTG Cuts at: 0 205 595 703 2646 Size: 205 390 108 1943 Fragments arranged by size: 1943 390 205 108 MspA1I CmG'CkG Cuts at: 0 27 748 2646 Size: 27 721 1898 Fragments arranged by size: 1898 721 27 NaeI GCC'GGC Cuts at: 0 1522 2646 Size: 1522 1124 NarI GG'CG_CC Cuts at: 0 2099 2646 Size: 2099 547 NdeI CA'TA_TG Cuts at: 0 2514 2646 Size: 2514 132 NgoMIV G'CCGG_C Cuts at: 0 1520 2646 Size: 1520 1126 NheI G'CTAG_C Cuts at: 0 717 2646 Size: 717 1929 PfoI T'CCnGG_A Cuts at: 0 121 1629 2444 2646 Size: 121 1508 815 202 Fragments arranged by size: 1508 815 202 121 PpuMI rG'GwC_Cy Cuts at: 0 1825 2646 Size: 1825 821 PsiI TTA'TAA Cuts at: 0 1705 2646 Size: 1705 941 PspOMI G'GGCC_C Cuts at: 0 2285 2646 Size: 2285 361 PvuII CAG'CTG Cuts at: 0 27 748 2646 Size: 27 721 1898 Fragments arranged by size: 1898 721 27 ScaI AGT'ACT Cuts at: 0 1872 2646 Size: 1872 774 SfcI C'TryA_G Cuts at: 0 1156 1918 2646 Size: 1156 762 728 Fragments arranged by size: 1156 762 728 SfoI GGC'GCC Cuts at: 0 2100 2646 Size: 2100 546 SmlI C'TyrA_G Cuts at: 0 398 2646 Size: 398 2248 SnaBI TAC'GTA Cuts at: 0 1611 1623 2646 Size: 1611 12 1023 Fragments arranged by size: 1611 1023 12 SpeI A'CTAG_T Cuts at: 0 577 2646 Size: 577 2069 SspI AAT'ATT Cuts at: 0 69 2646 Size: 69 2577 StyI C'CwwG_G Cuts at: 0 468 2613 2646 Size: 468 2145 33 Fragments arranged by size: 2145 468 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 19 2646 Size: 19 2627 TatI w'GTAC_w Cuts at: 0 1870 2646 Size: 1870 776 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1726 1758 2197 2229 2646 Size: 1726 32 439 32 417 Fragments arranged by size: 1726 439 417 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 594 2297 2646 Size: 594 1703 349 Fragments arranged by size: 1703 594 349 XhoI C'TCGA_G Cuts at: 0 398 2646 Size: 398 2248 XmnI GAAnn'nnTTC Cuts at: 0 393 450 890 1043 2646 Size: 393 57 440 153 1603 Fragments arranged by size: 1603 440 393 153 57 Enzymes that do cut: AclI AcuI AleI AlwNI ApaI ApaLI ApoI AseI AvaI BamHI BanI BanII BbeI BciVI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI PfoI PpuMI PsiI PspOMI PvuII ScaI SfcI SfoI SmlI SnaBI SpeI SspI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflII AflIII AgeI AhdI AlfI AloI AscI AsiSI AvrII BaeI BbsI BbvCI BcgI BclI BfrBI BglI BlpI BmgBI BpuEI BsaI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrGI BssHII BssSI BstBI BstZ17I BtgZI BtsI ClaI CspCI DrdI EagI EciI EcoICRI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NcoI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SphI SrfI StuI SwaI Tth111I XbaI XmaI ZraI