Restriction map for
pFN21AE4003
(Circular) (Six-base) MAPSORT of: pFN21AE4003.seq Check: 2538 from: 1 to: 5503
pFN21AE4003 5503 bp
With 182 enzymes: SgfI *
March 28, 2012 13:08 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3313 278
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2304 2846 1937
Size: 367 542 4594
Fragments arranged by size:
4594 542 367
Acc65I G'GTAC_C
Cuts at: 2824 2824
Size: 5503
AclI AA'CG_TT
Cuts at: 1563 3630 4003 1563
Size: 2067 373 3063
Fragments arranged by size:
3063 2067 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2711 2804 3566 4950 5218 1632
Size: 1079 93 762 1384 268 1917
Fragments arranged by size:
1917 1384 1079 762 268 93
AfeI AGC'GCT
Cuts at: 2261 2261
Size: 5503
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5306
AflIII A'CryG_T
Cuts at: 1203 4402 5379 1203
Size: 3199 977 1327
Fragments arranged by size:
3199 1327 977
AgeI A'CCGG_T
Cuts at: 4334 4334
Size: 5503
AhdI GACnn_n'nnGTC
Cuts at: 4232 4232
Size: 5503
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5503
AlwNI CAG_nnn'CTG
Cuts at: 2253 3246 4818 2253
Size: 993 1572 2938
Fragments arranged by size:
2938 1572 993
ApaI G_GGCC'C
Cuts at: 2278 2614 2749 2278
Size: 336 135 5032
Fragments arranged by size:
5032 336 135
ApaLI G'TGCA_C
Cuts at: 3560 4716 3560
Size: 1156 4347
ApoI r'AATT_y
Cuts at: 1493 2812 3038 3190 5299 1493
Size: 1319 226 152 2109 1697
Fragments arranged by size:
2109 1697 1319 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4057 160
Size: 872 3025 1606
Fragments arranged by size:
3025 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 2742 2828 1870
Size: 73 117 682 86 4545
Fragments arranged by size:
4545 682 117 86 73
BamHI G'GATC_C
Cuts at: 2833 3200 2833
Size: 367 5136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2085 2475 2764 2824 4279
Size: 325 217 627 298 390 289 60 1455
Cuts at: 4279 618
Size: 1842
Fragments arranged by size:
1842 1455 627 390 325 298 289 217
60
BanII G_rGCy'C
Cuts at: 1391 2278 2576 2614 2749 2822 1391
Size: 887 298 38 135 73 4072
Fragments arranged by size:
4072 887 298 135 73 38
BbeI G_GCGC'C
Cuts at: 2089 2768 2089
Size: 679 4824
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2521 928
Size: 971 622 3910
Fragments arranged by size:
3910 971 622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2477 2511 3694 3728 1903
Size: 34 540 34 1183 34 3678
Fragments arranged by size:
3678 1183 540 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3395 4605 3395
Size: 1210 4293
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5503
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4114 136
Size: 107 122 71 631 605 2442 1525
Fragments arranged by size:
2442 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2796 5498 2796
Size: 2702 2801
BlpI GC'TnA_GC
Cuts at: 2920 2920
Size: 5503
Bme1580I G_kGCm'C
Cuts at: 1790 2278 2614 2749 3564 4720 1790
Size: 488 336 135 815 1156 2573
Fragments arranged by size:
2573 1156 815 488 336 135
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5503
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2281 3234 4192
Size: 416 53 83 399 24 864 953 958
Cuts at: 4192 442
Size: 1753
Fragments arranged by size:
1753 958 953 864 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 2585 4163 1239
Size: 1124 222 1578 2579
Fragments arranged by size:
2579 1578 1124 222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3628 4493 4791 3628
Size: 865 298 4340
Fragments arranged by size:
4340 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2497 4166 882
Size: 617 998 1669 2219
Fragments arranged by size:
2219 1669 998 617
BsaAI yAC'GTr
Cuts at: 493 1204 2518 3294 493
Size: 711 1314 776 2702
Fragments arranged by size:
2702 1314 776 711
BsaBI GATnn'nnATC
Cuts at: 5497 5497
Size: 5503
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2086 2765 3310 3692
Size: 53 83 186 936 553 679 545 382
Cuts at: 3692 4327 275
Size: 635 1451
Fragments arranged by size:
1451 936 679 635 553 545 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3936 4334 4608 4755 5166 3936
Size: 398 274 147 411 4273
Fragments arranged by size:
4273 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2660 2690 1178
Size: 30 165 30 1257 30 3991
Fragments arranged by size:
3991 1257 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 2618 1186
Size: 1012 420 4071
Fragments arranged by size:
4071 1012 420
BseYI C'CCAG_C
Cuts at: 2398 4706 5202 2398
Size: 2308 496 2699
Fragments arranged by size:
2699 2308 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2540 2644 1163
Size: 573 804 104 4022
Fragments arranged by size:
4022 804 573 104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2953 3714 4742 1540
Size: 275 186 952 761 1028 2301
Fragments arranged by size:
2301 1028 952 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2517 3564 3649 4720 2517
Size: 1047 85 1071 3300
Fragments arranged by size:
3300 1071 1047 85
BsmI GAATG_Cn'
Cuts at: 3014 3107 3014
Size: 93 5410
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2278 2517 2576 2614 2749 2822 3564
Size: 399 488 239 59 38 135 73 742
Cuts at: 3564 3649 4720 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 488 399 239 135 85
73 59 38
BspEI T'CCGG_A
Cuts at: 5166 5166
Size: 5503
BspHI T'CATG_A
Cuts at: 3392 5122 3392
Size: 1730 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2457 2858 844
Size: 1613 401 3489
Fragments arranged by size:
3489 1613 401
BsrBI CCG'CTC
Cuts at: 1394 1823 2632 3390 1394
Size: 429 809 758 3507
Fragments arranged by size:
3507 809 758 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3998 4172 65
Size: 1183 2750 174 1396
Fragments arranged by size:
2750 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4147 4334 1700
Size: 110 2337 187 2869
Fragments arranged by size:
2869 2337 187 110
BsrGI T'GTAC_A
Cuts at: 96 2295 96
Size: 2199 3304
BssHII G'CGCG_C
Cuts at: 1925 2052 1925
Size: 127 5376
BssSI C'ACGA_G
Cuts at: 2534 3563 4575 2534
Size: 1029 1012 3462
Fragments arranged by size:
3462 1029 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5503
BstBI TT'CG_AA
Cuts at: 4307 4307
Size: 5503
BstEII G'GTnAC_C
Cuts at: 1184 2725 1184
Size: 1541 3962
BstYI r'GATC_y
Cuts at: 1972 2796 2833 3200 3585 3602 5054 5140 5152
Size: 824 37 367 385 17 1452 86 12
Cuts at: 5152 5498 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 824 385 367 346 86 37
17 12
Bsu36I CC'TnA_GG
Cuts at: 2213 2213
Size: 5503
BtgI C'CryG_G
Cuts at: 513 1065 2089 513
Size: 552 1024 3927
Fragments arranged by size:
3927 1024 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4764
Fragments arranged by size:
4764 683 56
BtsI GCAGTG_nn'
Cuts at: 2226 2547 2634 3019 3813 3833 2226
Size: 321 87 385 794 20 3896
Fragments arranged by size:
3896 794 385 321 87 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5468
DraI TTT'AAA
Cuts at: 2807 2807
Size: 5503
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4510 809
Size: 507 3194 1802
Fragments arranged by size:
3194 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2652 2950
Size: 54 1425 50 275 18 243 579 298
Cuts at: 2950 3839 5468 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 579 298 275 243 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2950 1537
Size: 275 1138 4090
Fragments arranged by size:
4090 1138 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2564 2597 3433 1353
Size: 1211 33 836 3423
Fragments arranged by size:
3423 1211 836 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4090 4464 4610 387
Size: 1064 138 2501 374 146 1280
Fragments arranged by size:
2501 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 2585 2711 2804 3566 4163 4950
Size: 393 731 222 126 93 762 597 787
Cuts at: 4950 5218 1239
Size: 268 1524
Fragments arranged by size:
1524 787 762 731 597 393 268 222
126 93
EcoO109I rG'GnC_Cy
Cuts at: 2610 2611 2626 2610
Size: 1 15 5487
Fragments arranged by size:
5487 15 1
EcoRI G'AATT_C
Cuts at: 2812 3190 2812
Size: 378 5125
FspI TGC'GCA
Cuts at: 4009 4009
Size: 5503
HaeII r_GCGC'y
Cuts at: 1678 2089 2263 2768 4650 1678
Size: 411 174 505 1882 2531
Fragments arranged by size:
2531 1882 505 411 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4144 4176 4218 4250 1373
Size: 32 135 32 2572 32 42 32 2626
Fragments arranged by size:
2626 2572 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2068 2305 2847 3093 669
Size: 988 281 130 237 542 246 3079
Fragments arranged by size:
3079 988 542 281 246 237 130
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5503
HpaI GTT'AAC
Cuts at: 3093 3093
Size: 5503
KasI G'GCGC_C
Cuts at: 2085 2764 2085
Size: 679 4824
KpnI G_GTAC'C
Cuts at: 2828 2828
Size: 5503
MfeI C'AATT_G
Cuts at: 3102 3102
Size: 5503
MluI A'CGCG_T
Cuts at: 5379 5379
Size: 5503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4617 4801 1305
Size: 3312 184 2007
Fragments arranged by size:
3312 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2075 2654 10
Size: 54 1425 343 243 579 2859
Fragments arranged by size:
2859 1425 579 343 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3461 3820 3979 518
Size: 648 2295 359 159 2042
Fragments arranged by size:
2295 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2040 2091 2658 2919 3596 4744 4989 1606
Size: 434 51 567 261 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 567 434 261 245 51
NarI GG'CG_CC
Cuts at: 2086 2765 2086
Size: 679 4824
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4951
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5503
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5503
NotI GC'GGCC_GC
Cuts at: 2950 2950
Size: 5503
NspI r_CATG'y
Cuts at: 1132 1229 2528 2861 4406 1132
Size: 97 1299 333 1545 2229
Fragments arranged by size:
2229 1545 1299 333 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5503
PciI A'CATG_T
Cuts at: 4402 4402
Size: 5503
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5503
PmeI GTTT'AAAC
Cuts at: 2807 2807
Size: 5503
PmlI CAC'GTG
Cuts at: 2518 2518
Size: 5503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 5085 5117 3572
Size: 32 1481 32 3958
Fragments arranged by size:
3958 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5503
PsiI TTA'TAA
Cuts at: 3073 3073
Size: 5503
PspOMI G'GGCC_C
Cuts at: 2274 2610 2745 2274
Size: 336 135 5032
Fragments arranged by size:
5032 336 135
PspXI vC'TCGA_Gb
Cuts at: 2060 2060
Size: 5503
PstI C_TGCA'G
Cuts at: 830 2233 2855 830
Size: 1403 622 3478
Fragments arranged by size:
3478 1403 622
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5503
PvuII CAG'CTG
Cuts at: 2658 2658
Size: 5503
SacII CC_GC'GG
Cuts at: 2092 2092
Size: 5503
SalI G'TCGA_C
Cuts at: 1936 2303 2845 1936
Size: 367 542 4594
Fragments arranged by size:
4594 542 367
SapI GCTCTTCn'nnn_
Cuts at: 2564 2564
Size: 5503
SbfI CC_TGCA'GG
Cuts at: 2855 2855
Size: 5503
ScaI AGT'ACT
Cuts at: 3751 5463 3751
Size: 1712 3791
SfcI C'TryA_G
Cuts at: 826 1045 2173 2229 2851 3986 4667 4858 826
Size: 219 1128 56 622 1135 681 191 1471
Fragments arranged by size:
1471 1135 1128 681 622 219 191 56
SfoI GGC'GCC
Cuts at: 2087 2766 2087
Size: 679 4824
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5503
SmaI CCC'GGG
Cuts at: 1872 2744 2830 1872
Size: 872 86 4545
Fragments arranged by size:
4545 872 86
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2060 3607 4508 4770 820
Size: 197 926 117 1547 901 262 1553
Fragments arranged by size:
1553 1547 926 901 262 197 117
SnaBI TAC'GTA
Cuts at: 493 3294 493
Size: 2801 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5503
SphI G_CATG'C
Cuts at: 1132 2861 1132
Size: 1729 3774
SspI AAT'ATT
Cuts at: 5 52 3427 5
Size: 47 3375 2081
Fragments arranged by size:
3375 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5503
StyI C'CwwG_G
Cuts at: 513 1065 2439 2942 513
Size: 552 1374 503 3074
Fragments arranged by size:
3074 1374 552 503
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2463 3530 3547 3700 3885 4330 325
Size: 1255 883 1067 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1067 883 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2295 2424 3749
Size: 274 80 33 51 1443 318 129 1325
Cuts at: 3749 5461 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1325 318 274 138 129 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5463 5495 5463
Size: 32 5471
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5503
XbaI T'CTAG_A
Cuts at: 2839 2839
Size: 5503
XhoI C'TCGA_G
Cuts at: 1943 2060 1943
Size: 117 5386
XmaI C'CCGG_G
Cuts at: 1870 2742 2828 1870
Size: 872 86 4545
Fragments arranged by size:
4545 872 86
XmnI GAAnn'nnTTC
Cuts at: 2789 3632 2789
Size: 843 4660
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3311 276
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PpuMI PsrI RsrII SacI SanDI SexAI
SfiI SrfI SwaI XcmI