(Circular) (Six-base) MAPSORT of: pFN21AE2078.seq Check: 8325 from: 1 to: 6949 pFN21AE2078 6949 bp With 182 enzymes: SgfI * May 14, 2013 11:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4759 278 Size: 53 83 186 936 3223 2468 Fragments arranged by size: 3223 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4292 1937 Size: 2355 4594 Acc65I G'GTAC_C Cuts at: 4270 4270 Size: 6949 AclI AA'CG_TT Cuts at: 1563 5076 5449 1563 Size: 3513 373 3063 Fragments arranged by size: 3513 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2634 2997 3844 5012 6396 6664 1632 Size: 1002 363 847 1168 1384 268 1917 Fragments arranged by size: 1917 1384 1168 1002 847 363 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6752 AflIII A'CryG_T Cuts at: 1203 3746 4246 5848 6825 1203 Size: 2543 500 1602 977 1327 Fragments arranged by size: 2543 1602 1327 977 500 AgeI A'CCGG_T Cuts at: 5780 5780 Size: 6949 AhdI GACnn_n'nnGTC Cuts at: 5678 5678 Size: 6949 AleI CACnn'nnGTG Cuts at: 1166 2145 3685 3861 1166 Size: 979 1540 176 4254 Fragments arranged by size: 4254 1540 979 176 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6949 AlwNI CAG_nnn'CTG Cuts at: 3641 3839 4692 6264 3641 Size: 198 853 1572 4326 Fragments arranged by size: 4326 1572 853 198 ApaLI G'TGCA_C Cuts at: 3199 5006 6162 3199 Size: 1807 1156 3986 Fragments arranged by size: 3986 1807 1156 ApoI r'AATT_y Cuts at: 1493 2258 2914 3220 3296 4258 4484 4636 6745 Size: 765 656 306 76 962 226 152 2109 Cuts at: 6745 1493 Size: 1697 Fragments arranged by size: 2109 1697 962 765 656 306 226 152 76 AseI AT'TA_AT Cuts at: 160 1032 3693 5503 160 Size: 872 2661 1810 1606 Fragments arranged by size: 2661 1810 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6949 AvaI C'yCGr_G Cuts at: 1870 1943 4215 4274 1870 Size: 73 2272 59 4545 Fragments arranged by size: 4545 2272 73 59 BamHI G'GATC_C Cuts at: 3173 3890 4279 4646 3173 Size: 717 389 367 5476 Fragments arranged by size: 5476 717 389 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4270 5725 618 Size: 325 217 627 2483 1455 1842 Fragments arranged by size: 2483 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4002 4268 1391 Size: 2611 266 4072 Fragments arranged by size: 4072 2611 266 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2178 2215 2947 928 Size: 971 279 37 732 4930 Fragments arranged by size: 4930 971 732 279 37 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5140 5174 1903 Size: 34 3203 34 3678 Fragments arranged by size: 3678 3203 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4841 6051 4841 Size: 1210 5739 BclI T'GATC_A Cuts at: 1548 2689 4068 1548 Size: 1141 1379 4429 Fragments arranged by size: 4429 1379 1141 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5560 136 Size: 107 122 71 631 605 3888 1525 Fragments arranged by size: 3888 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6944 6944 Size: 6949 BlpI GC'TnA_GC Cuts at: 4366 4366 Size: 6949 Bme1580I G_kGCm'C Cuts at: 1790 3203 5010 6166 1790 Size: 1413 1807 1156 2573 Fragments arranged by size: 2573 1807 1413 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6949 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4680 5638 442 Size: 416 53 83 399 24 3263 958 1753 Fragments arranged by size: 3263 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6949 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4194 4194 Size: 6949 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3045 5609 1239 Size: 1806 2564 2579 Fragments arranged by size: 2579 2564 1806 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2580 3356 5074 5939 6237 2580 Size: 776 1718 865 298 3292 Fragments arranged by size: 3292 1718 865 776 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3117 4168 5612 882 Size: 617 1618 1051 1444 2219 Fragments arranged by size: 2219 1618 1444 1051 617 BsaAI yAC'GTr Cuts at: 493 1204 3747 4740 493 Size: 711 2543 993 2702 Fragments arranged by size: 2702 2543 993 711 BsaBI GATnn'nnATC Cuts at: 3465 6943 3465 Size: 3478 3471 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4756 5138 5773 275 Size: 53 83 186 936 3223 382 635 1451 Fragments arranged by size: 3223 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2792 5382 5780 6054 6201 6612 2792 Size: 2590 398 274 147 411 3129 Fragments arranged by size: 3129 2590 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3731 3761 1178 Size: 30 165 30 2328 30 4366 Fragments arranged by size: 4366 2328 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 3752 4128 4183 4186 1186 Size: 838 1728 376 55 3 3949 Fragments arranged by size: 3949 1728 838 376 55 3 BseYI C'CCAG_C Cuts at: 3634 4200 6152 6648 3634 Size: 566 1952 496 3935 Fragments arranged by size: 3935 1952 566 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2544 3532 3726 3863 3958 1163 Size: 573 808 988 194 137 95 4154 Fragments arranged by size: 4154 988 808 573 194 137 95 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4399 5160 6188 1540 Size: 275 186 2398 761 1028 2301 Fragments arranged by size: 2398 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3010 3203 4002 5010 5095 6166 3010 Size: 193 799 1008 85 1071 3793 Fragments arranged by size: 3793 1071 1008 799 193 85 BsmI GAATG_Cn' Cuts at: 3455 3551 4460 4553 3455 Size: 96 909 93 5851 Fragments arranged by size: 5851 909 96 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4237 4237 Size: 6949 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3010 3203 4002 4268 5010 5095 6166 Size: 399 1220 193 799 266 742 85 1071 Cuts at: 6166 1391 Size: 2174 Fragments arranged by size: 2174 1220 1071 799 742 399 266 193 85 BspEI T'CCGG_A Cuts at: 2792 6612 2792 Size: 3820 3129 BspHI T'CATG_A Cuts at: 2473 2929 4838 6568 2473 Size: 456 1909 1730 2854 Fragments arranged by size: 2854 1909 1730 456 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3698 4304 844 Size: 2854 606 3489 Fragments arranged by size: 3489 2854 606 BsrBI CCG'CTC Cuts at: 1394 1823 4836 1394 Size: 429 3013 3507 Fragments arranged by size: 3507 3013 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3707 4011 5444 5618 65 Size: 1183 2459 304 1433 174 1396 Fragments arranged by size: 2459 1433 1396 1183 304 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5593 5780 1700 Size: 110 3783 187 2869 Fragments arranged by size: 3783 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3536 96 Size: 3440 3509 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6949 BssSI C'ACGA_G Cuts at: 2394 2718 5009 6021 2394 Size: 324 2291 1012 3322 Fragments arranged by size: 3322 2291 1012 324 BstAPI GCAn_nnn'nTGC Cuts at: 794 3548 794 Size: 2754 4195 BstBI TT'CG_AA Cuts at: 5753 5753 Size: 6949 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6949 BstXI CCAn_nnnn'nTGG Cuts at: 2204 2396 2567 2204 Size: 192 171 6586 Fragments arranged by size: 6586 192 171 BstYI r'GATC_y Cuts at: 1972 3173 3890 4279 4646 5031 5048 6500 6586 Size: 1201 717 389 367 385 17 1452 86 Cuts at: 6586 6598 6944 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1201 717 389 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2511 2511 Size: 6949 BtgI C'CryG_G Cuts at: 513 1065 2004 2203 2350 513 Size: 552 939 199 147 5112 Fragments arranged by size: 5112 939 552 199 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6210 Fragments arranged by size: 6210 683 56 BtsI GCAGTG_nn' Cuts at: 3851 4108 4465 5259 5279 3851 Size: 257 357 794 20 5521 Fragments arranged by size: 5521 794 357 257 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6949 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6914 DraI TTT'AAA Cuts at: 2079 2263 2704 4164 4253 2079 Size: 184 441 1460 89 4775 Fragments arranged by size: 4775 1460 441 184 89 DraIII CAC_nnn'GTG Cuts at: 3548 3548 Size: 6949 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5956 809 Size: 507 4640 1802 Fragments arranged by size: 4640 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3370 4396 5285 Size: 54 1425 50 275 18 1540 1026 889 Cuts at: 5285 6914 8 Size: 1629 43 Fragments arranged by size: 1629 1540 1425 1026 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4396 1537 Size: 275 2584 4090 Fragments arranged by size: 4090 2584 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2225 3338 4879 1353 Size: 872 1113 1541 3423 Fragments arranged by size: 3423 1541 1113 872 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5536 5910 6056 387 Size: 1064 138 3947 374 146 1280 Fragments arranged by size: 3947 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4000 4000 Size: 6949 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2634 2997 3045 3844 5012 5609 6396 Size: 393 1002 363 48 799 1168 597 787 Cuts at: 6396 6664 1239 Size: 268 1524 Fragments arranged by size: 1524 1168 1002 799 787 597 393 363 268 48 EcoO109I rG'GnC_Cy Cuts at: 2857 3527 2857 Size: 670 6279 EcoRI G'AATT_C Cuts at: 2914 4258 4636 2914 Size: 1344 378 5227 Fragments arranged by size: 5227 1344 378 EcoRV GAT'ATC Cuts at: 2742 2742 Size: 6949 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3091 3091 Size: 6949 FspI TGC'GCA Cuts at: 5455 5455 Size: 6949 HaeII r_GCGC'y Cuts at: 1678 6096 1678 Size: 4418 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2044 2076 2731 2763 4015 Size: 32 135 32 472 32 655 32 1252 Cuts at: 4015 4047 5590 5622 5664 5696 1373 Size: 32 1543 32 42 32 2626 Fragments arranged by size: 2626 1543 1252 655 472 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4293 4539 669 Size: 988 281 2355 246 3079 Fragments arranged by size: 3079 2355 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6949 HpaI GTT'AAC Cuts at: 4539 4539 Size: 6949 KpnI G_GTAC'C Cuts at: 4274 4274 Size: 6949 MfeI C'AATT_G Cuts at: 4548 4548 Size: 6949 MluI A'CGCG_T Cuts at: 4246 6825 4246 Size: 2579 4370 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2983 3139 6063 6247 1305 Size: 1678 156 2924 184 2007 Fragments arranged by size: 2924 2007 1678 184 156 MscI TGG'CCA Cuts at: 10 64 1489 1832 3372 10 Size: 54 1425 343 1540 3587 Fragments arranged by size: 3587 1540 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2145 2202 2325 2478 3685 3861 4076 Size: 648 979 57 123 153 1207 176 215 Cuts at: 4076 4907 5266 5425 518 Size: 831 359 159 2042 Fragments arranged by size: 2042 1207 979 831 648 359 215 176 159 153 123 57 MspA1I CmG'CkG Cuts at: 1606 2654 4210 4365 5042 6190 6435 1606 Size: 1048 1556 155 677 1148 245 2120 Fragments arranged by size: 2120 1556 1148 1048 677 245 155 NcoI C'CATG_G Cuts at: 513 1065 2004 2203 2350 513 Size: 552 939 199 147 5112 Fragments arranged by size: 5112 939 552 199 147 NdeI CA'TA_TG Cuts at: 387 387 Size: 6949 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6949 NotI GC'GGCC_GC Cuts at: 4396 4396 Size: 6949 NspI r_CATG'y Cuts at: 1132 1229 2609 4307 5852 1132 Size: 97 1380 1698 1545 2229 Fragments arranged by size: 2229 1698 1545 1380 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6949 PciI A'CATG_T Cuts at: 5848 5848 Size: 6949 PflMI CCAn_nnn'nTGG Cuts at: 1108 2888 1108 Size: 1780 5169 PmeI GTTT'AAAC Cuts at: 4253 4253 Size: 6949 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5018 5050 6531 6563 5018 Size: 32 1481 32 5404 Fragments arranged by size: 5404 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2857 3527 2857 Size: 670 6279 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6949 PsiI TTA'TAA Cuts at: 4519 4519 Size: 6949 PstI C_TGCA'G Cuts at: 830 4301 830 Size: 3471 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6949 PvuII CAG'CTG Cuts at: 2654 2654 Size: 6949 SacI G_AGCT'C Cuts at: 4002 4002 Size: 6949 SalI G'TCGA_C Cuts at: 1936 4291 1936 Size: 2355 4594 SbfI CC_TGCA'GG Cuts at: 4301 4301 Size: 6949 ScaI AGT'ACT Cuts at: 2286 5197 6909 2286 Size: 2911 1712 2326 Fragments arranged by size: 2911 2326 1712 SfcI C'TryA_G Cuts at: 826 1045 3307 3427 4297 5432 6113 6304 826 Size: 219 2262 120 870 1135 681 191 1471 Fragments arranged by size: 2262 1471 1135 870 681 219 191 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6949 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6949 SmaI CCC'GGG Cuts at: 1872 4276 1872 Size: 2404 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2595 3335 5053 5954 6216 820 Size: 197 926 652 740 1718 901 262 1553 Fragments arranged by size: 1718 1553 926 901 740 652 262 197 SnaBI TAC'GTA Cuts at: 493 4740 493 Size: 4247 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6949 SphI G_CATG'C Cuts at: 1132 4307 1132 Size: 3175 3774 SspI AAT'ATT Cuts at: 5 52 2708 2887 4873 5 Size: 47 2656 179 1986 2081 Fragments arranged by size: 2656 2081 1986 179 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6949 StyI C'CwwG_G Cuts at: 513 1065 2004 2203 2350 4388 513 Size: 552 939 199 147 2038 3074 Fragments arranged by size: 3074 2038 939 552 199 147 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2457 2979 4976 4993 5146 5331 5776 Size: 1255 877 522 1997 17 153 185 445 Cuts at: 5776 325 Size: 1498 Fragments arranged by size: 1997 1498 1255 877 522 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2284 3536 3830 Size: 274 80 33 51 1443 307 1252 294 Cuts at: 3830 5195 6907 96 Size: 1365 1712 138 Fragments arranged by size: 1712 1443 1365 1252 307 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3731 3763 6909 6941 3731 Size: 32 3146 32 3739 Fragments arranged by size: 3739 3146 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6949 XbaI T'CTAG_A Cuts at: 2188 4285 2188 Size: 2097 4852 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2396 3380 2396 Size: 984 5965 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6949 XmaI C'CCGG_G Cuts at: 1870 4274 1870 Size: 2404 4545 XmnI GAAnn'nnTTC Cuts at: 5078 5078 Size: 6949 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4757 276 Size: 53 83 186 936 3223 2468 Fragments arranged by size: 3223 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI