################################## # NO CUT ENZYMES ( 191 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeII MaeIII MamI MflI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI Sse8387I SspI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AlwI 621 GGATC GGATC(4/5) AlwNI 330 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG BbsI 63 GAAGAC GAAGAC(2/6) BbvI 490 GCTGC GCAGC(8/12) BfaI 485 CTAG C^TAG BpmI 409 CTGGAG CTGGAG(16/14) BpuAI 63 GAAGAC GAAGAC(2/6) BseRI 433 GAGGAG GAGGAG(10/8) BsmAI 47 GAGAC GTCTC(1/5) Bsp1407I 155 TGTACA T^GTACA BspMI 206 ACCTGC ACCTGC(4/8) BsrGI 155 TGTACA T^GTACA BssKI 575 CCTGG ^CC[ACGT]GG BstF5I 174 GGATG GGATG(2/0) BstNI 575 CCTGG CC^[AT]GG EcoRII 575 CCTGG ^CC[AT]GG Fnu4HI 490 GCTGC GC^[ACGT]GC FokI 174 GGATG GGATG(9/13) HapII 172 CCGG C^CGG HpaII 172 CCGG C^CGG ItaI 490 GCTGC GC^[ACGT]GC MaeI 485 CTAG C^TAG MfeI 518 CAATTG C^AATTG MslI 117 CACAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 172 CCGG C^CGG MunI 518 CAATTG C^AATTG MvaI 575 CCTGG CC^[AT]GG MwoI 348 GCTACAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 178 GGAGCC GG[ACGT]^[ACGT]CC PleI 49 GACTC GAGTC(4/5) PstI 208 CTGCAG CTGCA^G ScrFI 575 CCTGG CC^[ACGT]GG SpeI 484 ACTAGT A^CTAGT SphI 357 GCATGC GCATG^C SspBI 155 TGTACA T^GTACA TseI 490 GCTGC G^C[AT]GC XcmI 81 CCAATATACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AcsI 97 AAATTT [GA]^AATT[CT] 109 AAATTT AfaI 73 GTAC GT^AC 156 GTAC ApoI 97 AAATTT [GA]^AATT[CT] 109 AAATTT AvaII 414 GGACC G^G[AT]CC 534 GGACC BsmFI 218 GGGAC GGGAC(10/14) 499 GGGAC Cac8I 344 GCCAGC GC[ACGT]^[ACGT]GC 357 GCATGC EcoT22I 355 ATGCAT ATGCA^T 359 ATGCAT HaeIII 170 GGCC GG^CC 193 GGCC NsiI 355 ATGCAT ATGCA^T 359 ATGCAT NspI 357 GCATGC [GA]CATG^[CT] 652 GCATGT RsaI 73 GTAC GT^AC 156 GTAC SfcI 208 CTGCAG C^T[GA][CT]AG 349 CTACAG ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI BsrI Cfr13I DpnII HinfI MboI MboII MnlI MseI NdeII NlaIII Sau3AI Sau96I SfaNI TfiI Tru9I Tsp509I TspRI