################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BanI BanII BbeI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstZ17I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 196 CGCG CG^CG AlwNI 916 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 642 CCTGAGG CC^T[ACGT]AGG AspHI 199 GAGCAC G[AT]GC[AT]^C AvaII 481 GGACC G^G[AT]CC BbrPI 679 CACGTG CAC^GTG BciVI 952 GGATAC GTATCC(6/5) BsaAI 679 CACGTG [CT]AC^GT[GA] BsaI 667 GAGACC GGTCTC(1/5) BsiHKAI 199 GAGCAC G[AT]GC[AT]^C BsmAI 667 GAGAC GTCTC(1/5) BsrFI 181 ACCGGC [GA]^CCGG[CT] BstUI 196 CGCG CG^CG BstXI 545 CCACAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 642 CCTGAGG CC^T[ACGT]AGG Cfr10I 181 ACCGGC [GA]^CCGG[CT] DraII 480 AGGACCT [GA]G^G[ACGT]CC[CT] Eco81I 642 CCTGAGG CC^T[ACGT]AGG EcoO109I 480 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 930 GAATTC G^AATTC EcoT14I 411 CCTTGG C^C[AT][AT]GG HapII 182 CCGG C^CGG HincII 604 GTCAAC GT[CT]^[GA]AC HindII 604 GTCAAC GT[CT]^[GA]AC HindIII 362 AAGCTT A^AGCTT HpaII 182 CCGG C^CGG HphI 505 GGTGA GGTGA(8/7) MaeII 680 ACGT A^CGT MaeIII 337 GTGAC ^GT[ACGT]AC MseI 620 TTAA T^TAA MspI 182 CCGG C^CGG MvnI 196 CGCG CG^CG NspI 743 ACATGC [GA]CATG^[CT] PflMI 915 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 287 GAGTC GAGTC(4/5) PmaCI 679 CACGTG CAC^GTG PmlI 679 CACGTG CAC^GTG PpuMI 480 AGGACCT [GA]G^G[AT]CC[CT] PstI 565 CTGCAG CTGCA^G SexAI 1016 ACCTGGT A^CC[AT]GGT SfaNI 918 GATGC GCATC(5/9) SmlI 943 CTCAAG C^T[CT][GA]AG StyI 411 CCTTGG C^C[AT][AT]GG TfiI 784 GAATC G^A[AT]TC Tru9I 620 TTAA T^TAA Tsp45I 337 GTGAC ^GT[GC]AC Van91I 915 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 105 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 195 CCGC CCGC(-3/-1) 701 CCGC AcsI 649 GAATTT [GA]^AATT[CT] 930 GAATTC AfaI 213 GTAC GT^AC 418 GTAC ApoI 649 GAATTT [GA]^AATT[CT] 930 GAATTC Asp700I 97 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 684 GAAGAAGTTC BalI 368 TGGCCA TGG^CCA 725 TGGCCA BamHI 809 GGATCC G^GATCC 938 GGATCC BmyI 199 GAGCAC G[AGT]GC[ACT]^C 259 GTGCCC BsaJI 411 CCTTGG C^C[ACGT][ACGT]GG 818 CCCTGG BseRI 205 CTCCTC GAGGAG(10/8) 925 GAGGAG Bsp1286I 199 GAGCAC G[AGT]GC[ACT]^C 259 GTGCCC BsrDI 729 CATTGC GCAATG(2/0) 713 GCAATG EarI 76 GAAGAG CTCTTC(1/4) 1029 GAAGAG EcoNI 216 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 819 CCTGGTAGAGG HinfI 287 GAGTC G^A[ACGT]TC 784 GAATC Ksp632I 76 GAAGAG CTCTTC(1/4) 1029 GAAGAG MluNI 368 TGGCCA TGG^CCA 725 TGGCCA MscI 368 TGGCCA TGG^CCA 725 TGGCCA MslI 546 CACAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 675 CACTCACGTG RsaI 213 GTAC GT^AC 418 GTAC TaqI 150 TCGA T^CGA 446 TCGA TthHB8I 150 TCGA T^CGA 446 TCGA XmnI 97 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 684 GAAGAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AluI AlwI BbvI BfaI BpmI BsiYI BslI BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI HaeIII ItaI MaeI MboI MboII MflI MnlI MspA1I MvaI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfcI TseI Tsp509I TspRI XhoII