(Circular) (Six-base) MAPSORT of: pFN21AE3141.seq Check: 8928 from: 1 to: 5758 pFN21AE3141 5758 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3568 278 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3101 1937 Size: 1164 4594 Acc65I G'GTAC_C Cuts at: 3079 3079 Size: 5758 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3821 5205 5473 1632 Size: 2189 1384 268 1917 Fragments arranged by size: 2189 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5561 AflIII A'CryG_T Cuts at: 1203 4657 5634 1203 Size: 3454 977 1327 Fragments arranged by size: 3454 1327 977 AgeI A'CCGG_T Cuts at: 4589 4589 Size: 5758 AhdI GACnn_n'nnGTC Cuts at: 4487 4487 Size: 5758 AleI CACnn'nnGTG Cuts at: 1166 2555 2684 1166 Size: 1389 129 4240 Fragments arranged by size: 4240 1389 129 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5758 AlwNI CAG_nnn'CTG Cuts at: 2926 3501 5073 2926 Size: 575 1572 3611 Fragments arranged by size: 3611 1572 575 ApaLI G'TGCA_C Cuts at: 3815 4971 3815 Size: 1156 4602 ApoI r'AATT_y Cuts at: 1493 2654 2935 3067 3293 3445 5554 1493 Size: 1161 281 132 226 152 2109 1697 Fragments arranged by size: 2109 1697 1161 281 226 152 132 AseI AT'TA_AT Cuts at: 160 1032 4312 160 Size: 872 3280 1606 Fragments arranged by size: 3280 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 3083 1870 Size: 73 1140 4545 Fragments arranged by size: 4545 1140 73 BamHI G'GATC_C Cuts at: 2814 2943 3088 3455 2814 Size: 129 145 367 5117 Fragments arranged by size: 5117 367 145 129 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3079 4534 618 Size: 325 217 627 1292 1455 1842 Fragments arranged by size: 1842 1455 1292 627 325 217 BanII G_rGCy'C Cuts at: 1391 3077 1391 Size: 1686 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4787 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3949 3983 1903 Size: 34 2012 34 3678 Fragments arranged by size: 3678 2012 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2951 3650 4860 2951 Size: 699 1210 3849 Fragments arranged by size: 3849 1210 699 BclI T'GATC_A Cuts at: 1548 1548 Size: 5758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4369 136 Size: 107 122 71 631 605 2697 1525 Fragments arranged by size: 2697 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5753 5753 Size: 5758 BlpI GC'TnA_GC Cuts at: 3175 3175 Size: 5758 Bme1580I G_kGCm'C Cuts at: 1790 2268 3819 4975 1790 Size: 478 1551 1156 2573 Fragments arranged by size: 2573 1551 1156 478 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5758 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2820 3489 4447 Size: 416 53 83 399 24 1403 669 958 Cuts at: 4447 442 Size: 1753 Fragments arranged by size: 1753 1403 958 669 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5758 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2243 2295 2690 2948 4418 1239 Size: 812 192 52 395 258 1470 2579 Fragments arranged by size: 2579 1470 812 395 258 192 52 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2933 3883 4748 5046 2933 Size: 950 865 298 3645 Fragments arranged by size: 3645 950 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2666 4421 882 Size: 617 1167 1755 2219 Fragments arranged by size: 2219 1755 1167 617 BsaAI yAC'GTr Cuts at: 493 1204 2686 3549 493 Size: 711 1482 863 2702 Fragments arranged by size: 2702 1482 863 711 BsaBI GATnn'nnATC Cuts at: 5752 5752 Size: 5758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3565 3947 4582 275 Size: 53 83 186 936 2032 382 635 1451 Fragments arranged by size: 2032 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4191 4589 4863 5010 5421 4191 Size: 398 274 147 411 4528 Fragments arranged by size: 4528 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5533 Fragments arranged by size: 5533 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2201 2945 1186 Size: 1015 744 3999 Fragments arranged by size: 3999 1015 744 BseYI C'CCAG_C Cuts at: 4961 5457 4961 Size: 496 5262 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5185 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3208 3969 4997 1540 Size: 275 186 1207 761 1028 2301 Fragments arranged by size: 2301 1207 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2208 3819 3904 4975 2208 Size: 1611 85 1071 2991 Fragments arranged by size: 2991 1611 1071 85 BsmI GAATG_Cn' Cuts at: 3269 3362 3269 Size: 93 5665 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2208 2268 3077 3819 3904 4975 1391 Size: 399 418 60 809 742 85 1071 2174 Fragments arranged by size: 2174 1071 809 742 418 399 85 60 BspEI T'CCGG_A Cuts at: 5421 5421 Size: 5758 BspHI T'CATG_A Cuts at: 3647 5377 3647 Size: 1730 4028 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3113 844 Size: 2269 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3645 1394 Size: 429 1822 3507 Fragments arranged by size: 3507 1822 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2725 2733 4253 4427 65 Size: 1183 1477 8 1520 174 1396 Fragments arranged by size: 1520 1477 1396 1183 174 8 BsrFI r'CCGG_y Cuts at: 1700 1810 2186 4402 4589 1700 Size: 110 376 2216 187 2869 Fragments arranged by size: 2869 2216 376 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5758 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5758 BssSI C'ACGA_G Cuts at: 3818 4830 3818 Size: 1012 4746 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5758 BstBI TT'CG_AA Cuts at: 4562 4562 Size: 5758 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5758 BstXI CCAn_nnnn'nTGG Cuts at: 2557 2557 Size: 5758 BstYI r'GATC_y Cuts at: 1972 2122 2814 2943 3088 3455 3840 3857 5309 Size: 150 692 129 145 367 385 17 1452 Cuts at: 5309 5395 5407 5753 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 692 385 367 346 150 145 129 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2648 2648 Size: 5758 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4267 Fragments arranged by size: 4267 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5019 Fragments arranged by size: 5019 683 56 BtsI GCAGTG_nn' Cuts at: 3274 4068 4088 3274 Size: 794 20 4944 Fragments arranged by size: 4944 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2458 2493 529 Size: 35 1894 35 3794 Fragments arranged by size: 3794 1894 35 35 DraI TTT'AAA Cuts at: 3062 3062 Size: 5758 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4765 809 Size: 507 3449 1802 Fragments arranged by size: 3449 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2373 2703 2730 Size: 54 1425 50 275 18 543 330 27 Cuts at: 2730 3205 4094 5723 8 Size: 475 889 1629 43 Fragments arranged by size: 1629 1425 889 543 475 330 275 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3205 1537 Size: 275 1393 4090 Fragments arranged by size: 4090 1393 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 3029 3688 1353 Size: 723 953 659 3423 Fragments arranged by size: 3423 953 723 659 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4345 4719 4865 387 Size: 1064 138 2756 374 146 1280 Fragments arranged by size: 2756 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2243 2295 2690 2948 3821 4418 Size: 393 419 192 52 395 258 873 597 Cuts at: 4418 5205 5473 1239 Size: 787 268 1524 Fragments arranged by size: 1524 873 787 597 419 395 393 268 258 192 52 EcoNI CCTnn'n_nnAGG Cuts at: 2225 2828 2225 Size: 603 5155 EcoO109I rG'GnC_Cy Cuts at: 2486 2486 Size: 5758 EcoRI G'AATT_C Cuts at: 2935 3067 3445 2935 Size: 132 378 5248 Fragments arranged by size: 5248 378 132 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2078 2363 2078 Size: 285 5473 FspI TGC'GCA Cuts at: 4264 4264 Size: 5758 HaeII r_GCGC'y Cuts at: 1678 4905 1678 Size: 3227 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2283 2315 2665 2697 4399 Size: 32 135 32 711 32 350 32 1702 Cuts at: 4399 4431 4473 4505 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1702 711 350 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2611 3102 3348 669 Size: 988 281 673 491 246 3079 Fragments arranged by size: 3079 988 673 491 281 246 HindIII A'AGCT_T Cuts at: 748 2367 748 Size: 1619 4139 HpaI GTT'AAC Cuts at: 3348 3348 Size: 5758 KpnI G_GTAC'C Cuts at: 3083 3083 Size: 5758 MfeI C'AATT_G Cuts at: 3357 3357 Size: 5758 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2079 2666 4872 5056 1305 Size: 774 587 2206 184 2007 Fragments arranged by size: 2206 2007 774 587 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2375 2732 10 Size: 54 1425 343 543 357 3036 Fragments arranged by size: 3036 1425 543 357 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2555 2684 3716 4075 4234 518 Size: 648 1389 129 1032 359 159 2042 Fragments arranged by size: 2042 1389 1032 648 359 159 129 MspA1I CmG'CkG Cuts at: 1606 2147 2411 2900 2999 3174 3851 4999 5244 Size: 541 264 489 99 175 677 1148 245 Cuts at: 5244 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 541 489 264 245 175 99 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4267 Fragments arranged by size: 4267 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5758 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5758 NotI GC'GGCC_GC Cuts at: 3205 3205 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 2752 3116 4661 1132 Size: 97 1523 364 1545 2229 Fragments arranged by size: 2229 1545 1523 364 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5758 PciI A'CATG_T Cuts at: 4657 4657 Size: 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 2926 1108 Size: 1818 3940 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PmlI CAC'GTG Cuts at: 2686 2686 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3827 3859 5340 5372 3827 Size: 32 1481 32 4213 Fragments arranged by size: 4213 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2486 2486 Size: 5758 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5758 PsiI TTA'TAA Cuts at: 3328 3328 Size: 5758 PstI C_TGCA'G Cuts at: 830 2574 3110 830 Size: 1744 536 3478 Fragments arranged by size: 3478 1744 536 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 PvuII CAG'CTG Cuts at: 2147 2411 2900 2999 2147 Size: 264 489 99 4906 Fragments arranged by size: 4906 489 264 99 SalI G'TCGA_C Cuts at: 1936 3100 1936 Size: 1164 4594 SbfI CC_TGCA'GG Cuts at: 3110 3110 Size: 5758 ScaI AGT'ACT Cuts at: 4006 5718 4006 Size: 1712 4046 SexAI A'CCwGG_T Cuts at: 3021 3021 Size: 5758 SfcI C'TryA_G Cuts at: 826 1045 2316 2570 2901 3106 4241 4922 5113 Size: 219 1271 254 331 205 1135 681 191 Cuts at: 5113 826 Size: 1471 Fragments arranged by size: 1471 1271 1135 681 331 254 219 205 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5758 SmaI CCC'GGG Cuts at: 1872 3085 1872 Size: 1213 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2948 3862 4763 5025 820 Size: 197 926 1005 914 901 262 1553 Fragments arranged by size: 1553 1005 926 914 901 262 197 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5758 SphI G_CATG'C Cuts at: 1132 3116 1132 Size: 1984 3774 SspI AAT'ATT Cuts at: 5 52 3682 5 Size: 47 3630 2081 Fragments arranged by size: 3630 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5758 StyI C'CwwG_G Cuts at: 513 1065 2004 2416 3197 513 Size: 552 939 412 781 3074 Fragments arranged by size: 3074 939 781 552 412 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3066 3785 3802 3955 4140 4585 325 Size: 1255 1486 719 17 153 185 445 1498 Fragments arranged by size: 1498 1486 1255 719 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2422 4004 5716 Size: 274 80 33 51 1443 445 1582 1712 Cuts at: 5716 96 Size: 138 Fragments arranged by size: 1712 1582 1443 445 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5718 5750 5718 Size: 32 5726 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5758 XbaI T'CTAG_A Cuts at: 2110 3094 2110 Size: 984 4774 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5758 XmaI C'CCGG_G Cuts at: 1870 3083 1870 Size: 1213 4545 XmnI GAAnn'nnTTC Cuts at: 2106 2693 3887 2106 Size: 587 1194 3977 Fragments arranged by size: 3977 1194 587 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3566 276 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI