################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvaII AviII AvrII BalI BanI BanII BbeI BbrPI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 15 CGCG CG^CG AlwNI 1572 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 1681 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1665 GGATCC G^GATCC BbvCI 338 CCTCAGC CCTCAGC(-5/-2) BcgI 1803 GCACCCAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1410 GGATAC GTATCC(6/5) BglI 1111 GCCAATCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 195 GTGCCC G[AGT]GC[ACT]^C BsaBI 1549 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 105 GAGACC GGTCTC(1/5) BseRI 1600 CTCCTC GAGGAG(10/8) BsgI 808 CTGCAC GTGCAG(16/14) BsmI 1752 GCATTC GAATGC(1/-1) Bsp1286I 195 GTGCCC G[AGT]GC[ACT]^C BsrBI 1597 CCGCTC CCGCTC(-3/-3) BsrDI 1491 CATTGC GCAATG(2/0) BstAPI 1237 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1533 GGTGACC G^GT[ACGT]ACC BstPI 1533 GGTGACC G^GT[ACGT]ACC BstUI 15 CGCG CG^CG BstYI 1665 GGATCC [GA]^GATC[CT] Cac8I 830 GCAAGC GC[ACGT]^[ACGT]GC CfoI 14 GCGC GCG^C DraIII 1369 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 482 CCTACGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1533 GGTGACC G^GT[ACGT]ACC EcoT14I 308 CCAAGG C^C[AT][AT]GG HhaI 14 GCGC GCG^C HinP1I 14 GCGC G^CGC MaeII 1150 ACGT A^CGT MamI 1549 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1665 GGATCC [GA]^GATC[CT] MvnI 15 CGCG CG^CG PflFI 1681 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC SexAI 1645 ACCAGGT A^CC[AT]GGT SfcI 119 CTACAG C^T[GA][CT]AG SspI 945 AATATT AAT^ATT StyI 308 CCAAGG C^C[AT][AT]GG SwaI 890 ATTTAAAT ATTT^AAAT TaqI 1226 TCGA T^CGA Tth111I 1681 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1226 TCGA T^CGA XhoII 1665 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflIII 1540 ACATGT A^C[GA][CT]GT 1851 ACATGT BpmI 36 CTCCAG CTGGAG(16/14) 1826 CTGGAG BspLU11I 1540 ACATGT A^CATGT 1851 ACATGT DpnII 670 GATC ^GATC 1666 GATC DraI 796 TTTAAA TTT^AAA 891 TTTAAA EcoRI 219 GAATTC G^AATTC 1440 GAATTC FauI 1108 CCCGC CCCGC(4/6) 1841 GCGGG HapII 1117 CCGG C^CGG 1400 CCGG HpaII 1117 CCGG C^CGG 1400 CCGG MboI 670 GATC ^GATC 1666 GATC MslI 1267 CACTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1417 CATATTTATG MspI 1117 CCGG C^CGG 1400 CCGG NdeII 670 GATC ^GATC 1666 GATC NspI 1540 ACATGT [GA]CATG^[CT] 1851 ACATGT PvuII 341 CAGCTG CAG^CTG 1575 CAGCTG Sau3AI 670 GATC ^GATC 1666 GATC XcmI 449 CCACACCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1331 CCAGTTCTTCTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BbsI BbvI BfaI BpuAI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cfr13I DdeI EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboII MnlI MseI MspA1I MvaI MwoI NlaIII NlaIV PleI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I TspRI