(Circular) (Six-base) MAPSORT of: pFN21AE2385.seq Check: 9450 from: 1 to: 6583 pFN21AE2385 6583 bp With 182 enzymes: SgfI * November 30, 2011 11:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4393 278 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3926 1937 Size: 1989 4594 Acc65I G'GTAC_C Cuts at: 3904 3904 Size: 6583 AclI AA'CG_TT Cuts at: 1563 4710 5083 1563 Size: 3147 373 3063 Fragments arranged by size: 3147 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2525 2528 4646 6030 6298 1632 Size: 893 3 2118 1384 268 1917 Fragments arranged by size: 2118 1917 1384 893 268 3 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6386 AflIII A'CryG_T Cuts at: 1203 3545 3856 5482 6459 1203 Size: 2342 311 1626 977 1327 Fragments arranged by size: 2342 1626 1327 977 311 AgeI A'CCGG_T Cuts at: 5414 5414 Size: 6583 AhdI GACnn_n'nnGTC Cuts at: 5312 5312 Size: 6583 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6583 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6583 AlwNI CAG_nnn'CTG Cuts at: 3582 4326 5898 3582 Size: 744 1572 4267 Fragments arranged by size: 4267 1572 744 ApaLI G'TGCA_C Cuts at: 4640 5796 4640 Size: 1156 5427 ApoI r'AATT_y Cuts at: 1493 2224 2335 3008 3445 3567 3892 4118 4270 Size: 731 111 673 437 122 325 226 152 Cuts at: 4270 6379 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 731 673 437 325 226 152 122 111 AseI AT'TA_AT Cuts at: 160 1032 5137 160 Size: 872 4105 1606 Fragments arranged by size: 4105 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 AvaI C'yCGr_G Cuts at: 1870 1943 3908 1870 Size: 73 1965 4545 Fragments arranged by size: 4545 1965 73 BamHI G'GATC_C Cuts at: 3670 3913 4280 3670 Size: 243 367 5973 Fragments arranged by size: 5973 367 243 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3904 5359 618 Size: 325 217 627 2117 1455 1842 Fragments arranged by size: 2117 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3902 1391 Size: 2511 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2258 2790 3690 928 Size: 971 359 532 900 3821 Fragments arranged by size: 3821 971 900 532 359 BbvCI CC'TCA_GC Cuts at: 2344 2344 Size: 6583 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3797 3831 4774 4808 1903 Size: 34 1860 34 943 34 3678 Fragments arranged by size: 3678 1860 943 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3409 4475 5685 3409 Size: 1066 1210 4307 Fragments arranged by size: 4307 1210 1066 BclI T'GATC_A Cuts at: 1548 1548 Size: 6583 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3122 5194 136 Size: 107 122 71 631 605 1450 2072 1525 Fragments arranged by size: 2072 1525 1450 631 605 122 107 71 BglII A'GATC_T Cuts at: 6578 6578 Size: 6583 BlpI GC'TnA_GC Cuts at: 4000 4000 Size: 6583 Bme1580I G_kGCm'C Cuts at: 1790 2204 4644 5800 1790 Size: 414 2440 1156 2573 Fragments arranged by size: 2573 2440 1156 414 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6583 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3283 3371 3645 Size: 416 53 83 399 24 1866 88 274 Cuts at: 3645 4314 5272 442 Size: 669 958 1753 Fragments arranged by size: 1866 1753 958 669 416 399 274 88 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6583 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2613 3435 2613 Size: 822 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2026 3852 5243 1239 Size: 787 1826 1391 2579 Fragments arranged by size: 2579 1826 1391 787 Bpu10I CC'TnA_GC Cuts at: 2145 2344 2145 Size: 199 6384 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4708 5573 5871 4708 Size: 865 298 5420 Fragments arranged by size: 5420 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2104 5246 882 Size: 617 605 3142 2219 Fragments arranged by size: 3142 2219 617 605 BsaAI yAC'GTr Cuts at: 493 1204 4374 493 Size: 711 3170 2702 Fragments arranged by size: 3170 2702 711 BsaBI GATnn'nnATC Cuts at: 3558 6577 3558 Size: 3019 3564 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4390 4772 5407 275 Size: 53 83 186 936 2857 382 635 1451 Fragments arranged by size: 2857 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5016 5414 5688 5835 6246 5016 Size: 398 274 147 411 5353 Fragments arranged by size: 5353 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3051 3081 1178 Size: 30 165 30 1648 30 4680 Fragments arranged by size: 4680 1648 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3596 1186 Size: 2410 4173 BseYI C'CCAG_C Cuts at: 3039 5786 6282 3039 Size: 2747 496 3340 Fragments arranged by size: 3340 2747 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2798 1163 Size: 573 1062 4948 Fragments arranged by size: 4948 1062 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4033 4794 5822 1540 Size: 275 186 2032 761 1028 2301 Fragments arranged by size: 2301 2032 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4644 4729 5800 4644 Size: 85 1071 5427 Fragments arranged by size: 5427 1071 85 BsmI GAATG_Cn' Cuts at: 3757 4094 4187 3757 Size: 337 93 6153 Fragments arranged by size: 6153 337 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2204 3902 4644 4729 5800 1391 Size: 399 414 1698 742 85 1071 2174 Fragments arranged by size: 2174 1698 1071 742 414 399 85 BspEI T'CCGG_A Cuts at: 6246 6246 Size: 6583 BspHI T'CATG_A Cuts at: 4472 6202 4472 Size: 1730 4853 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3938 844 Size: 3094 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3604 4470 1394 Size: 429 1781 866 3507 Fragments arranged by size: 3507 1781 866 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3495 5078 5252 65 Size: 1183 2247 1583 174 1396 Fragments arranged by size: 2247 1583 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5227 5414 1700 Size: 110 3417 187 2869 Fragments arranged by size: 3417 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6583 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6583 BssSI C'ACGA_G Cuts at: 4643 5655 4643 Size: 1012 5571 BstAPI GCAn_nnn'nTGC Cuts at: 794 3248 794 Size: 2454 4129 BstBI TT'CG_AA Cuts at: 5387 5387 Size: 6583 BstEII G'GTnAC_C Cuts at: 1184 3538 1184 Size: 2354 4229 BstYI r'GATC_y Cuts at: 1972 3670 3913 4280 4665 4682 6134 6220 6232 Size: 1698 243 367 385 17 1452 86 12 Cuts at: 6232 6578 1972 Size: 346 1977 Fragments arranged by size: 1977 1698 1452 385 367 346 243 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6031 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2077 2411 3655 487 Size: 683 56 851 334 1244 3415 Fragments arranged by size: 3415 1244 851 683 334 56 BtsI GCAGTG_nn' Cuts at: 3399 4099 4893 4913 3399 Size: 700 794 20 5069 Fragments arranged by size: 5069 794 700 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6583 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3647 3682 529 Size: 35 3083 35 3430 Fragments arranged by size: 3430 3083 35 35 DraI TTT'AAA Cuts at: 2803 2898 3887 2803 Size: 95 989 5499 Fragments arranged by size: 5499 989 95 DraIII CAC_nnn'GTG Cuts at: 3379 3379 Size: 6583 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5590 809 Size: 507 4274 1802 Fragments arranged by size: 4274 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4030 4919 6548 Size: 54 1425 50 275 18 2200 889 1629 Cuts at: 6548 8 Size: 43 Fragments arranged by size: 2200 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4030 1537 Size: 275 2218 4090 Fragments arranged by size: 4090 2218 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2492 2547 2582 3186 4513 1353 Size: 1139 55 35 604 1327 3423 Fragments arranged by size: 3423 1327 1139 604 55 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2051 3493 5170 5544 5690 387 Size: 1064 138 462 1442 1677 374 146 1280 Fragments arranged by size: 1677 1442 1280 1064 462 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2026 2525 2528 3852 4646 5243 6030 Size: 393 394 499 3 1324 794 597 787 Cuts at: 6030 6298 1239 Size: 268 1524 Fragments arranged by size: 1524 1324 794 787 597 499 394 393 268 3 EcoNI CCTnn'n_nnAGG Cuts at: 2491 2491 Size: 6583 EcoRI G'AATT_C Cuts at: 2224 3445 3892 4270 2224 Size: 1221 447 378 4537 Fragments arranged by size: 4537 1221 447 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3041 3480 3041 Size: 439 6144 FspI TGC'GCA Cuts at: 5089 5089 Size: 6583 HaeII r_GCGC'y Cuts at: 1678 5730 1678 Size: 4052 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2272 2304 5224 5256 5298 Size: 32 135 32 700 32 2920 32 42 Cuts at: 5298 5330 1373 Size: 32 2626 Fragments arranged by size: 2920 2626 700 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3927 4173 669 Size: 988 281 1989 246 3079 Fragments arranged by size: 3079 1989 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6583 HpaI GTT'AAC Cuts at: 4173 4173 Size: 6583 KpnI G_GTAC'C Cuts at: 3908 3908 Size: 6583 MfeI C'AATT_G Cuts at: 4182 4182 Size: 6583 MluI A'CGCG_T Cuts at: 6459 6459 Size: 6583 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2729 3001 5697 5881 1305 Size: 1424 272 2696 184 2007 Fragments arranged by size: 2696 2007 1424 272 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4761 Fragments arranged by size: 4761 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3276 3426 4541 4900 5059 518 Size: 648 2110 150 1115 359 159 2042 Fragments arranged by size: 2110 2042 1115 648 359 159 150 MspA1I CmG'CkG Cuts at: 1606 2348 3582 3846 3999 4676 5824 6069 1606 Size: 742 1234 264 153 677 1148 245 2120 Fragments arranged by size: 2120 1234 1148 742 677 264 245 153 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6031 NdeI CA'TA_TG Cuts at: 387 387 Size: 6583 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6583 NotI GC'GGCC_GC Cuts at: 4030 4030 Size: 6583 NspI r_CATG'y Cuts at: 1132 1229 3549 3860 3941 5486 1132 Size: 97 2320 311 81 1545 2229 Fragments arranged by size: 2320 2229 1545 311 97 81 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6583 PciI A'CATG_T Cuts at: 3545 3856 5482 3545 Size: 311 1626 4646 Fragments arranged by size: 4646 1626 311 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6583 PfoI T'CCnGG_A Cuts at: 3449 3449 Size: 6583 PmeI GTTT'AAAC Cuts at: 3887 3887 Size: 6583 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4652 4684 6165 6197 4652 Size: 32 1481 32 5038 Fragments arranged by size: 5038 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6583 PsiI TTA'TAA Cuts at: 4153 4153 Size: 6583 PstI C_TGCA'G Cuts at: 830 3935 830 Size: 3105 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6583 PvuII CAG'CTG Cuts at: 2348 3582 2348 Size: 1234 5349 SalI G'TCGA_C Cuts at: 1936 3925 1936 Size: 1989 4594 SbfI CC_TGCA'GG Cuts at: 3935 3935 Size: 6583 ScaI AGT'ACT Cuts at: 4831 6543 4831 Size: 1712 4871 SexAI A'CCwGG_T Cuts at: 3650 3650 Size: 6583 SfcI C'TryA_G Cuts at: 826 1045 2124 3931 5066 5747 5938 826 Size: 219 1079 1807 1135 681 191 1471 Fragments arranged by size: 1807 1471 1135 1079 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6583 SmaI CCC'GGG Cuts at: 1872 3910 1872 Size: 2038 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4687 5588 5850 820 Size: 197 926 2744 901 262 1553 Fragments arranged by size: 2744 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4374 493 Size: 3881 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6583 SphI G_CATG'C Cuts at: 1132 3941 1132 Size: 2809 3774 SspI AAT'ATT Cuts at: 5 52 2952 4507 5 Size: 47 2900 1555 2081 Fragments arranged by size: 2900 2081 1555 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6583 StyI C'CwwG_G Cuts at: 513 1065 2313 4022 513 Size: 552 1248 1709 3074 Fragments arranged by size: 3074 1709 1248 552 SwaI ATTT'AAAT Cuts at: 2898 2898 Size: 6583 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3390 3825 4610 4627 4780 4965 5410 Size: 1255 1810 435 785 17 153 185 445 Cuts at: 5410 325 Size: 1498 Fragments arranged by size: 1810 1498 1255 785 445 435 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4829 6541 96 Size: 274 80 33 51 1443 2852 1712 138 Fragments arranged by size: 2852 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3738 3770 6543 6575 3738 Size: 32 2773 32 3746 Fragments arranged by size: 3746 2773 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3689 1316 Size: 2373 4210 XbaI T'CTAG_A Cuts at: 3919 3919 Size: 6583 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2461 3343 2461 Size: 882 5701 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6583 XmaI C'CCGG_G Cuts at: 1870 3908 1870 Size: 2038 4545 XmnI GAAnn'nnTTC Cuts at: 4712 4712 Size: 6583 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4391 276 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI