Restriction map for
pFN21AE2385
(Linear) (Six-base) MAPSORT of: KIEE2385.seq Check: 1042 from: 1 to: 1878
KIEE2385.orf
With 182 enzymes: SgfI *
November 30, 2011 10:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 523 1878
Size: 520 3 1355
Fragments arranged by size:
1355 520 3
AflIII A'CryG_T
Cuts at: 0 1540 1851 1878
Size: 1540 311 27
Fragments arranged by size:
1540 311 27
AlwNI CAG_nnn'CTG
Cuts at: 0 1577 1878
Size: 1577 301
ApoI r'AATT_y
Cuts at: 0 219 330 1003 1440 1562 1878
Size: 219 111 673 437 122 316
Fragments arranged by size:
673 437 316 219 122 111
BamHI G'GATC_C
Cuts at: 0 1665 1878
Size: 1665 213
BbsI GAAGACnn'nnnn_
Cuts at: 0 253 785 1685 1878
Size: 253 532 900 193
Fragments arranged by size:
900 532 253 193
BbvCI CC'TCA_GC
Cuts at: 0 339 1878
Size: 339 1539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1792 1826 1878
Size: 1792 34 52
Fragments arranged by size:
1792 52 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1404 1878
Size: 1404 474
BglI GCCn_nnn'nGGC
Cuts at: 0 1117 1878
Size: 1117 761
Bme1580I G_kGCm'C
Cuts at: 0 199 1878
Size: 199 1679
BmrI ACTGGGnnnn_n'
Cuts at: 0 1278 1366 1640 1878
Size: 1278 88 274 238
Fragments arranged by size:
1278 274 238 88
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 608 1430 1878
Size: 608 822 448
Fragments arranged by size:
822 608 448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 1847 1878
Size: 21 1826 31
Fragments arranged by size:
1826 31 21
Bpu10I CC'TnA_GC
Cuts at: 0 140 339 1878
Size: 140 199 1539
Fragments arranged by size:
1539 199 140
BsaI GGTCTCn'nnnn_
Cuts at: 0 99 1878
Size: 99 1779
BsaBI GATnn'nnATC
Cuts at: 0 1553 1878
Size: 1553 325
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1046 1076 1878
Size: 1046 30 802
Fragments arranged by size:
1046 802 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1591 1878
Size: 1591 287
BseYI C'CCAG_C
Cuts at: 0 1034 1878
Size: 1034 844
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1878
Size: 793 1085
BsmI GAATG_Cn'
Cuts at: 0 1752 1878
Size: 1752 126
Bsp1286I G_dGCh'C
Cuts at: 0 199 1878
Size: 199 1679
BsrBI CCG'CTC
Cuts at: 0 1599 1878
Size: 1599 279
BsrDI GCAATG_nn'
Cuts at: 0 1490 1878
Size: 1490 388
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1243 1878
Size: 1243 635
BstEII G'GTnAC_C
Cuts at: 0 1533 1878
Size: 1533 345
BstYI r'GATC_y
Cuts at: 0 1665 1878
Size: 1665 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 72 406 1650 1878
Size: 72 334 1244 228
Fragments arranged by size:
1244 334 228 72
BtsI GCAGTG_nn'
Cuts at: 0 1394 1878
Size: 1394 484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1642 1677 1878
Size: 1642 35 201
Fragments arranged by size:
1642 201 35
DraI TTT'AAA
Cuts at: 0 798 893 1878
Size: 798 95 985
Fragments arranged by size:
985 798 95
DraIII CAC_nnn'GTG
Cuts at: 0 1374 1878
Size: 1374 504
EarI CTCTTCn'nnn_
Cuts at: 0 487 542 577 1181 1878
Size: 487 55 35 604 697
Fragments arranged by size:
697 604 487 55 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 1488 1878
Size: 46 1442 390
Fragments arranged by size:
1442 390 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 520 523 1847 1878
Size: 21 499 3 1324 31
Fragments arranged by size:
1324 499 31 21 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 486 1878
Size: 486 1392
EcoRI G'AATT_C
Cuts at: 0 219 1440 1878
Size: 219 1221 438
Fragments arranged by size:
1221 438 219
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1036 1475 1878
Size: 1036 439 403
Fragments arranged by size:
1036 439 403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 267 299 1878
Size: 267 32 1579
Fragments arranged by size:
1579 267 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 996 1878
Size: 724 272 882
Fragments arranged by size:
882 724 272
MslI CAynn'nnrTG
Cuts at: 0 1271 1421 1878
Size: 1271 150 457
Fragments arranged by size:
1271 457 150
MspA1I CmG'CkG
Cuts at: 0 343 1577 1841 1878
Size: 343 1234 264 37
Fragments arranged by size:
1234 343 264 37
NspI r_CATG'y
Cuts at: 0 1544 1855 1878
Size: 1544 311 23
Fragments arranged by size:
1544 311 23
PciI A'CATG_T
Cuts at: 0 1540 1851 1878
Size: 1540 311 27
Fragments arranged by size:
1540 311 27
PfoI T'CCnGG_A
Cuts at: 0 1444 1878
Size: 1444 434
PvuII CAG'CTG
Cuts at: 0 343 1577 1878
Size: 343 1234 301
Fragments arranged by size:
1234 343 301
SexAI A'CCwGG_T
Cuts at: 0 1645 1878
Size: 1645 233
SfcI C'TryA_G
Cuts at: 0 119 1878
Size: 119 1759
SspI AAT'ATT
Cuts at: 0 947 1878
Size: 947 931
StyI C'CwwG_G
Cuts at: 0 308 1878
Size: 308 1570
SwaI ATTT'AAAT
Cuts at: 0 893 1878
Size: 893 985
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1385 1820 1878
Size: 1385 435 58
Fragments arranged by size:
1385 435 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1733 1765 1878
Size: 1733 32 113
Fragments arranged by size:
1733 113 32
Tth111I GACn'n_nGTC
Cuts at: 0 1684 1878
Size: 1684 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 456 1338 1878
Size: 456 882 540
Fragments arranged by size:
882 540 456
Enzymes that do cut:
AcuI AflIII AlwNI ApoI BamHI BbsI BbvCI BcgI
BciVI BglI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaBI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BsrBI
BsrDI BstAPI BstEII BstYI BtgZI BtsI CspCI DraI
DraIII EarI EciI Eco57MI EcoNI EcoRI FalI Hin4I
MmeI MslI MspA1I NspI PciI PfoI PvuII SexAI
SfcI SspI StyI SwaI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BanII BbeI BclI
BfrBI BglII BlpI BmgBI BmtI BpuEI BsaAI BsaHI
BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI
BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I
BtgI ClaI DrdI EaeI EagI EcoICRI EcoO109I EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI TatI XbaI
XhoI XmaI XmnI ZraI