################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AclI 647 AACGTT AA^CGTT AocI 276 CCTCAGG CC^T[ACGT]AGG Asp700I 595 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 532 GGATCC G^GATCC BbsI 1045 GAAGAC GAAGAC(2/6) BcnI 59 CCCGG CC^[GC]GG BglI 97 GCCCTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1045 GAAGAC GAAGAC(2/6) BsaI 1153 GGTCTC GGTCTC(1/5) BsgI 887 GTGCAG GTGCAG(16/14) BsiEI 136 CGGCCG CG[GA][CT]^CG BsrFI 138 GCCGGT [GA]^CCGG[CT] Bsu36I 276 CCTCAGG CC^T[ACGT]AGG Cfr10I 138 GCCGGT [GA]^CCGG[CT] DsaI 344 CCATGG C^C[GA][CT]GG EaeI 136 CGGCCG [CT]^GGCC[GA] EagI 136 CGGCCG C^GGCCG EarI 202 CTCTTC CTCTTC(1/4) EclXI 136 CGGCCG C^GGCCG Eco52I 136 CGGCCG C^GGCCG Eco81I 276 CCTCAGG CC^T[ACGT]AGG EcoNI 19 CCTCTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 102 GCGGG CCCGC(4/6) Ksp632I 202 CTCTTC CTCTTC(1/4) MspA1I 121 CAGCGG C[AC]G^C[GT]G NciI 59 CCCGG CC^[GC]GG NcoI 344 CCATGG C^CATGG PleI 176 GACTC GAGTC(4/5) PpuMI 1037 AGGTCCT [GA]G^G[AT]CC[CT] Psp1406I 647 AACGTT AA^CGTT SacI 7 GAGCTC GAGCT^C SmlI 537 CTCAAG C^T[CT][GA]AG SphI 951 GCATGC GCATG^C StuI 726 AGGCCT AGG^CCT Tsp45I 522 GTCAC ^GT[GC]AC Tsp509I 1136 AATT ^AATT XmnI 595 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AspHI 7 GAGCTC G[AT]GC[AT]^C 544 GAGCAC BanII 7 GAGCTC G[GA]GC[CT]^C 309 GGGCTC BfaI 65 CTAG C^TAG 475 CTAG BseRI 4 GAGGAG GAGGAG(10/8) 478 GAGGAG BsiHKAI 7 GAGCTC G[AT]GC[AT]^C 544 GAGCAC BstYI 495 AGATCC [GA]^GATC[CT] 532 GGATCC DpnII 496 GATC ^GATC 533 GATC DraII 183 AGGCCCT [GA]G^G[ACGT]CC[CT] 1037 AGGTCCT EcoO109I 183 AGGCCCT [GA]G^G[ACGT]CC[CT] 1037 AGGTCCT HgaI 91 GACGC GACGC(5/10) 1110 GCGTC HphI 141 GGTGA GGTGA(8/7) 1128 TCACC MaeI 65 CTAG C^TAG 475 CTAG MaeII 633 ACGT A^CGT 648 ACGT MaeIII 522 GTCAC ^GT[ACGT]AC 1094 GTTAC MboI 496 GATC ^GATC 533 GATC MflI 495 AGATCC [GA]^GATC[CT] 532 GGATCC MseI 1004 TTAA T^TAA 1148 TTAA MslI 901 CATGCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 952 CATGCCCGTG NdeII 496 GATC ^GATC 533 GATC NspI 900 ACATGC [GA]CATG^[CT] 951 GCATGC PflMI 181 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 300 CCACTGCCTGG Sau3AI 496 GATC ^GATC 533 GATC SfcI 659 CTGTAG C^T[GA][CT]AG 668 CTGTAG Tru9I 1004 TTAA T^TAA 1148 TTAA Van91I 181 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 300 CCACTGCCTGG XcmI 181 CCAGGCCCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 665 CCACTGTAGAACTGG XhoII 495 AGATCC [GA]^GATC[CT] 532 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BbvI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboII MnlI MspI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI StyI TfiI TseI TspRI