(Circular) (Six-base) MAPSORT of: pFN21AE3511.seq Check: 6721 from: 1 to: 5896 pFN21AE3511 5896 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3706 278 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3239 1937 Size: 1302 4594 Acc65I G'GTAC_C Cuts at: 3217 3217 Size: 5896 AclI AA'CG_TT Cuts at: 1563 2653 4023 4396 1563 Size: 1090 1370 373 3063 Fragments arranged by size: 3063 1370 1090 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2418 2466 2868 3959 5343 5611 1632 Size: 786 48 402 1091 1384 268 1917 Fragments arranged by size: 1917 1384 1091 786 402 268 48 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5699 AflIII A'CryG_T Cuts at: 1203 4795 5772 1203 Size: 3592 977 1327 Fragments arranged by size: 3592 1327 977 AgeI A'CCGG_T Cuts at: 4727 4727 Size: 5896 AhdI GACnn_n'nnGTC Cuts at: 4625 4625 Size: 5896 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5896 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5896 AlwNI CAG_nnn'CTG Cuts at: 2192 2614 2663 3639 5211 2192 Size: 422 49 976 1572 2877 Fragments arranged by size: 2877 1572 976 422 49 ApaLI G'TGCA_C Cuts at: 3953 5109 3953 Size: 1156 4740 ApoI r'AATT_y Cuts at: 1493 3205 3431 3583 5692 1493 Size: 1712 226 152 2109 1697 Fragments arranged by size: 2109 1712 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4450 160 Size: 872 3418 1606 Fragments arranged by size: 3418 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 AvaI C'yCGr_G Cuts at: 1870 1943 3221 1870 Size: 73 1278 4545 Fragments arranged by size: 4545 1278 73 BamHI G'GATC_C Cuts at: 2537 3226 3593 2537 Size: 689 367 4840 Fragments arranged by size: 4840 689 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3217 4672 618 Size: 325 217 627 1430 1455 1842 Fragments arranged by size: 1842 1455 1430 627 325 217 BanII G_rGCy'C Cuts at: 1391 2016 2318 3215 1391 Size: 625 302 897 4072 Fragments arranged by size: 4072 897 625 302 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3057 928 Size: 971 1158 3767 Fragments arranged by size: 3767 1158 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4087 4121 1903 Size: 34 2150 34 3678 Fragments arranged by size: 3678 2150 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3788 4998 3788 Size: 1210 4686 BclI T'GATC_A Cuts at: 1548 1548 Size: 5896 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2108 4507 136 Size: 107 122 71 631 605 436 2399 1525 Fragments arranged by size: 2399 1525 631 605 436 122 107 71 BglII A'GATC_T Cuts at: 5891 5891 Size: 5896 BlpI GC'TnA_GC Cuts at: 3313 3313 Size: 5896 Bme1580I G_kGCm'C Cuts at: 1790 3957 5113 1790 Size: 2167 1156 2573 Fragments arranged by size: 2573 2167 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5896 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2221 3627 4585 Size: 416 53 83 399 24 804 1406 958 Cuts at: 4585 442 Size: 1753 Fragments arranged by size: 1753 1406 958 804 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5896 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2054 2054 Size: 5896 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2000 3015 3188 4556 1239 Size: 761 1015 173 1368 2579 Fragments arranged by size: 2579 1368 1015 761 173 Bpu10I CC'TnA_GC Cuts at: 2084 2084 Size: 5896 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2527 4021 4886 5184 2527 Size: 1494 865 298 3239 Fragments arranged by size: 3239 1494 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3164 4559 882 Size: 617 1665 1395 2219 Fragments arranged by size: 2219 1665 1395 617 BsaAI yAC'GTr Cuts at: 493 1204 3687 493 Size: 711 2483 2702 Fragments arranged by size: 2702 2483 711 BsaBI GATnn'nnATC Cuts at: 5890 5890 Size: 5896 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3703 4085 4720 275 Size: 53 83 186 936 2170 382 635 1451 Fragments arranged by size: 2170 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4329 4727 5001 5148 5559 4329 Size: 398 274 147 411 4666 Fragments arranged by size: 4666 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2442 2472 2514 2544 1178 Size: 30 165 30 1039 30 42 30 4530 Fragments arranged by size: 4530 1039 165 42 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2498 1186 Size: 838 474 4584 Fragments arranged by size: 4584 838 474 BseYI C'CCAG_C Cuts at: 2362 2995 5099 5595 2362 Size: 633 2104 496 2663 Fragments arranged by size: 2663 2104 633 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2913 1163 Size: 573 1177 4146 Fragments arranged by size: 4146 1177 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2144 3346 4107 5135 1540 Size: 275 186 143 1202 761 1028 2301 Fragments arranged by size: 2301 1202 1028 761 275 186 143 BsiHKAI G_wGCw'C Cuts at: 2016 2553 3957 4042 5113 2016 Size: 537 1404 85 1071 2799 Fragments arranged by size: 2799 1404 1071 537 85 BsmI GAATG_Cn' Cuts at: 3407 3500 3407 Size: 93 5803 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2318 2553 3215 3957 4042 5113 Size: 399 226 302 235 662 742 85 1071 Cuts at: 5113 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 662 399 302 235 226 85 BspEI T'CCGG_A Cuts at: 5559 5559 Size: 5896 BspHI T'CATG_A Cuts at: 3785 5515 3785 Size: 1730 4166 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3251 844 Size: 2407 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3783 1394 Size: 429 1960 3507 Fragments arranged by size: 3507 1960 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4391 4565 65 Size: 1183 3143 174 1396 Fragments arranged by size: 3143 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2143 4540 4727 1700 Size: 110 333 2397 187 2869 Fragments arranged by size: 2869 2397 333 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5896 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5896 BssSI C'ACGA_G Cuts at: 3956 4968 3956 Size: 1012 4884 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5896 BstBI TT'CG_AA Cuts at: 4700 4700 Size: 5896 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5896 BstYI r'GATC_y Cuts at: 1972 2500 2537 3226 3593 3978 3995 5447 5533 Size: 528 37 689 367 385 17 1452 86 Cuts at: 5533 5545 5891 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 689 528 385 367 346 86 37 17 12 Bsu36I CC'TnA_GG Cuts at: 2282 2282 Size: 5896 BtgI C'CryG_G Cuts at: 513 1065 2004 2349 513 Size: 552 939 345 4060 Fragments arranged by size: 4060 939 552 345 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5157 Fragments arranged by size: 5157 683 56 BtsI GCAGTG_nn' Cuts at: 2305 2612 3412 4206 4226 2305 Size: 307 800 794 20 3975 Fragments arranged by size: 3975 800 794 307 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5896 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5861 DraI TTT'AAA Cuts at: 3200 3200 Size: 5896 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4903 809 Size: 507 3587 1802 Fragments arranged by size: 3587 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2141 3343 4232 Size: 54 1425 50 275 18 311 1202 889 Cuts at: 4232 5861 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1202 889 311 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2141 3343 1537 Size: 275 329 1202 4090 Fragments arranged by size: 4090 1202 329 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2213 3826 1353 Size: 860 1613 3423 Fragments arranged by size: 3423 1613 860 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4483 4857 5003 387 Size: 1064 138 2894 374 146 1280 Fragments arranged by size: 2894 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2014 2014 Size: 5896 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2000 2418 2466 2868 3015 3188 3959 Size: 393 368 418 48 402 147 173 771 Cuts at: 3959 4556 5343 5611 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 771 597 418 402 393 368 268 173 147 48 EcoNI CCTnn'n_nnAGG Cuts at: 2028 2028 Size: 5896 EcoO109I rG'GnC_Cy Cuts at: 2189 3043 2189 Size: 854 5042 EcoRI G'AATT_C Cuts at: 3205 3583 3205 Size: 378 5518 FspI TGC'GCA Cuts at: 4402 4402 Size: 5896 HaeII r_GCGC'y Cuts at: 1678 2114 2247 2332 5043 1678 Size: 436 133 85 2711 2531 Fragments arranged by size: 2711 2531 436 133 85 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2444 2474 2476 2506 2514 Size: 32 135 32 872 30 2 30 8 Cuts at: 2514 2546 2819 2851 4537 4569 4611 4643 1373 Size: 32 273 32 1686 32 42 32 2626 Fragments arranged by size: 2626 1686 872 273 135 42 32 32 32 32 32 32 30 30 8 2 HincII GTy'rAC Cuts at: 669 1657 1938 3240 3486 669 Size: 988 281 1302 246 3079 Fragments arranged by size: 3079 1302 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5896 HpaI GTT'AAC Cuts at: 3486 3486 Size: 5896 KpnI G_GTAC'C Cuts at: 3221 3221 Size: 5896 MfeI C'AATT_G Cuts at: 3495 3495 Size: 5896 MluI A'CGCG_T Cuts at: 5772 5772 Size: 5896 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5010 5194 1305 Size: 3705 184 2007 Fragments arranged by size: 3705 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4074 Fragments arranged by size: 4074 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2910 2961 3854 4213 4372 518 Size: 648 1744 51 893 359 159 2042 Fragments arranged by size: 2042 1744 893 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2128 3312 3989 5137 5382 1606 Size: 522 1184 677 1148 245 2120 Fragments arranged by size: 2120 1184 1148 677 522 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2349 513 Size: 552 939 345 4060 Fragments arranged by size: 4060 939 552 345 NdeI CA'TA_TG Cuts at: 387 387 Size: 5896 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5896 NotI GC'GGCC_GC Cuts at: 3343 3343 Size: 5896 NspI r_CATG'y Cuts at: 1132 1229 2909 2960 3254 4799 1132 Size: 97 1680 51 294 1545 2229 Fragments arranged by size: 2229 1680 1545 294 97 51 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5896 PciI A'CATG_T Cuts at: 4795 4795 Size: 5896 PflMI CCAn_nnn'nTGG Cuts at: 1108 2192 2311 1108 Size: 1084 119 4693 Fragments arranged by size: 4693 1084 119 PfoI T'CCnGG_A Cuts at: 2016 3045 3063 3165 2016 Size: 1029 18 102 4747 Fragments arranged by size: 4747 1029 102 18 PmeI GTTT'AAAC Cuts at: 3200 3200 Size: 5896 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2589 2621 3965 3997 5478 5510 2589 Size: 32 1344 32 1481 32 2975 Fragments arranged by size: 2975 1481 1344 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3043 3043 Size: 5896 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5896 PsiI TTA'TAA Cuts at: 3466 3466 Size: 5896 PstI C_TGCA'G Cuts at: 830 3248 830 Size: 2418 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5896 SacI G_AGCT'C Cuts at: 2016 2016 Size: 5896 SalI G'TCGA_C Cuts at: 1936 3238 1936 Size: 1302 4594 SbfI CC_TGCA'GG Cuts at: 3248 3248 Size: 5896 ScaI AGT'ACT Cuts at: 4144 5856 4144 Size: 1712 4184 SfcI C'TryA_G Cuts at: 826 1045 2664 2673 3244 4379 5060 5251 826 Size: 219 1619 9 571 1135 681 191 1471 Fragments arranged by size: 1619 1471 1135 681 571 219 191 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5896 SmaI CCC'GGG Cuts at: 1872 3223 1872 Size: 1351 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2542 4000 4901 5163 820 Size: 197 926 599 1458 901 262 1553 Fragments arranged by size: 1553 1458 926 901 599 262 197 SnaBI TAC'GTA Cuts at: 493 3687 493 Size: 3194 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5896 SphI G_CATG'C Cuts at: 1132 2960 3254 1132 Size: 1828 294 3774 Fragments arranged by size: 3774 1828 294 SspI AAT'ATT Cuts at: 5 52 3820 5 Size: 47 3768 2081 Fragments arranged by size: 3768 2081 47 StuI AGG'CCT Cuts at: 1517 2733 1517 Size: 1216 4680 StyI C'CwwG_G Cuts at: 513 1065 2004 2158 2349 2518 3335 513 Size: 552 939 154 191 169 817 3074 Fragments arranged by size: 3074 939 817 552 191 169 154 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3923 3940 4093 4278 4723 325 Size: 1255 2343 17 153 185 445 1498 Fragments arranged by size: 2343 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4142 5854 96 Size: 274 80 33 51 1443 2165 1712 138 Fragments arranged by size: 2165 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2252 2284 5856 5888 2252 Size: 32 3572 32 2260 Fragments arranged by size: 3572 2260 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5896 XbaI T'CTAG_A Cuts at: 3232 3232 Size: 5896 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2193 2677 2193 Size: 484 5412 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5896 XmaI C'CCGG_G Cuts at: 1870 3221 1870 Size: 1351 4545 XmnI GAAnn'nnTTC Cuts at: 2604 4025 2604 Size: 1421 4475 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3704 276 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI