################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccII AcyI AflII AflIII AgeI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco52I Eco81I EcoO65I EcoRV FseI FspI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MluNI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI PvuII RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspI SwaI Tsp45I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccIII 425 TCCGGA T^CCGGA AclI 892 AACGTT AA^CGTT AhdI 316 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 234 GGATC GGATC(4/5) AlwNI 16 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 98 AGCGCT AGC^GCT AspEI 316 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 478 GAGCTC G[AT]GC[AT]^C BanI 682 GGCACC G^G[CT][GA]CC BciVI 576 GGATAC GTATCC(6/5) BclI 396 TGATCA T^GATCA BcnI 177 CCGGG CC^[GC]GG BglI 49 GCCTCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 464 AGATCT A^GATCT BsaWI 425 TCCGGA [AT]^CCGG[AT] BseAI 425 TCCGGA T^CCGGA BseRI 328 GAGGAG GAGGAG(10/8) BsgI 470 GTGCAG GTGCAG(16/14) BsiHKAI 478 GAGCTC G[AT]GC[AT]^C Bsp1407I 41 TGTACA T^GTACA BspEI 425 TCCGGA T^CCGGA BsrGI 41 TGTACA T^GTACA BstAPI 15 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 224 CCATCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 464 AGATCT [GA]^GATC[CT] Cac8I 107 GCCTGC GC[ACGT]^[ACGT]GC DdeI 206 CTGAG C^T[ACGT]AG DsaI 230 CCGTGG C^C[GA][CT]GG EaeI 238 CGGCCA [CT]^GGCC[GA] Eam1105I 316 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 98 AGCGCT AGC^GCT EcoRI 166 GAATTC G^AATTC EcoT14I 53 CCAAGG C^C[AT][AT]GG EcoT22I 854 ATGCAT ATGCA^T FauI 84 GCGGG CCCGC(4/6) FbaI 396 TGATCA T^GATCA HaeII 98 AGCGCT [GA]GCGC^[CT] HgaI 721 GACGC GACGC(5/10) HincII 715 GTCAAC GT[CT]^[GA]AC HindII 715 GTCAAC GT[CT]^[GA]AC HphI 914 TCACC GGTGA(8/7) MflI 464 AGATCT [GA]^GATC[CT] MroI 425 TCCGGA T^CCGGA NciI 177 CCGGG CC^[GC]GG NsiI 854 ATGCAT ATGCA^T PflMI 379 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 892 AACGTT AA^CGTT SacI 478 GAGCTC GAGCT^C SbfI 603 CCTGCAGG CCTGCA^GG SfaNI 702 GATGC GCATC(5/9) SmlI 481 CTCAAG C^T[CT][GA]AG Sse8387I 603 CCTGCAGG CCTGCA^GG SspBI 41 TGTACA T^GTACA StuI 638 AGGCCT AGG^CCT StyI 53 CCAAGG C^C[AT][AT]GG TaqI 794 TCGA T^CGA TfiI 728 GATTC G^A[AT]TC TthHB8I 794 TCGA T^CGA Van91I 379 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 464 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 166 GAATTC [GA]^AATT[CT] 650 AAATTT ApoI 166 GAATTC [GA]^AATT[CT] 650 AAATTT BanII 148 GAGCCC G[GA]GC[CT]^C 478 GAGCTC BbsI 525 GAAGAC GAAGAC(2/6) 181 GTCTTC BpuAI 525 GAAGAC GAAGAC(2/6) 181 GTCTTC BsaJI 53 CCAAGG C^C[ACGT][ACGT]GG 230 CCGTGG BsmFI 93 GTCCC GGGAC(10/14) 369 GTCCC BspMI 20 ACCTGC ACCTGC(4/8) 602 ACCTGC BstF5I 171 CATCC GGATG(2/0) 867 GGATG CfoI 99 GCGC GCG^C 568 GCGC DraII 488 AGGTCCT [GA]G^G[ACGT]CC[CT] 518 AGGACCT EcoNI 21 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 201 CCTCTCTGAGG EcoO109I 488 AGGTCCT [GA]G^G[ACGT]CC[CT] 518 AGGACCT FokI 171 CATCC GGATG(9/13) 867 GGATG HhaI 99 GCGC GCG^C 568 GCGC HinP1I 99 GCGC G^CGC 568 GCGC MaeII 125 ACGT A^CGT 893 ACGT MseI 494 TTAA T^TAA 635 TTAA NlaIV 197 GGAACC GG[ACGT]^[ACGT]CC 682 GGCACC NspI 80 ACATGC [GA]CATG^[CT] 836 GCATGT PleI 322 GAGTC GAGTC(4/5) 547 GAGTC PpuMI 488 AGGTCCT [GA]G^G[AT]CC[CT] 518 AGGACCT Tru9I 494 TTAA T^TAA 635 TTAA TspRI 242 CAGTG CA[GC]TG[ACGT][ACGT]^ 542 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AfaI AluI Asp700I AvaII BbvI BmyI BpmI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cfr13I DpnII EcoRII Fnu4HI HaeIII HapII HinfI HpaII ItaI MboI MboII MnlI MslI MspI MvaI MwoI NdeII NlaIII PstI RsaI Sau3AI Sau96I ScrFI SfcI TseI Tsp509I XmnI