(Circular) (Six-base) MAPSORT of: pFN21AE5356.seq Check: 3838 from: 1 to: 5632 pFN21AE5356 5632 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2034 2034 Size: 5632 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3442 278 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2975 1937 Size: 1038 4594 Acc65I G'GTAC_C Cuts at: 2953 2953 Size: 5632 AclI AA'CG_TT Cuts at: 1563 2898 3759 4132 1563 Size: 1335 861 373 3063 Fragments arranged by size: 3063 1335 861 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2549 3695 5079 5347 1632 Size: 917 1146 1384 268 1917 Fragments arranged by size: 1917 1384 1146 917 268 AfeI AGC'GCT Cuts at: 2105 2105 Size: 5632 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5435 AflIII A'CryG_T Cuts at: 1203 4531 5508 1203 Size: 3328 977 1327 Fragments arranged by size: 3328 1327 977 AgeI A'CCGG_T Cuts at: 4463 4463 Size: 5632 AhdI GACnn_n'nnGTC Cuts at: 2326 4361 2326 Size: 2035 3597 AleI CACnn'nnGTG Cuts at: 1166 2507 1166 Size: 1341 4291 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5632 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2828 2860 2828 Size: 32 5600 AlwNI CAG_nnn'CTG Cuts at: 2026 3375 4947 2026 Size: 1349 1572 2711 Fragments arranged by size: 2711 1572 1349 ApaLI G'TGCA_C Cuts at: 3689 4845 3689 Size: 1156 4476 ApoI r'AATT_y Cuts at: 1493 2171 2655 2941 3167 3319 5428 1493 Size: 678 484 286 226 152 2109 1697 Fragments arranged by size: 2109 1697 678 484 286 226 152 AseI AT'TA_AT Cuts at: 160 1032 4186 160 Size: 872 3154 1606 Fragments arranged by size: 3154 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 AvaI C'yCGr_G Cuts at: 1870 1943 2957 1870 Size: 73 1014 4545 Fragments arranged by size: 4545 1014 73 BamHI G'GATC_C Cuts at: 2962 3329 2962 Size: 367 5265 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2687 2953 4408 618 Size: 325 217 627 900 266 1455 1842 Fragments arranged by size: 1842 1455 900 627 325 266 217 BanII G_rGCy'C Cuts at: 1391 2157 2487 2951 1391 Size: 766 330 464 4072 Fragments arranged by size: 4072 766 464 330 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2179 2537 928 Size: 971 280 358 4023 Fragments arranged by size: 4023 971 358 280 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3823 3857 1903 Size: 34 50 34 1802 34 3678 Fragments arranged by size: 3678 1802 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2575 3524 4734 2575 Size: 949 1210 3473 Fragments arranged by size: 3473 1210 949 BclI T'GATC_A Cuts at: 1548 2401 1548 Size: 853 4779 BfrBI ATG'CAT Cuts at: 2861 2861 Size: 5632 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2060 4243 136 Size: 107 122 71 631 605 388 2183 1525 Fragments arranged by size: 2183 1525 631 605 388 122 107 71 BglII A'GATC_T Cuts at: 2469 5627 2469 Size: 3158 2474 BlpI GC'TnA_GC Cuts at: 3049 3049 Size: 5632 Bme1580I G_kGCm'C Cuts at: 1790 2142 3693 4849 1790 Size: 352 1551 1156 2573 Fragments arranged by size: 2573 1551 1156 352 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5632 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3363 4321 442 Size: 416 53 83 399 24 1946 958 1753 Fragments arranged by size: 1946 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2570 2855 2924 4292 1239 Size: 1331 285 69 1368 2579 Fragments arranged by size: 2579 1368 1331 285 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2471 3757 4622 4920 2471 Size: 1286 865 298 3183 Fragments arranged by size: 3183 1286 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4295 882 Size: 617 2796 2219 Fragments arranged by size: 2796 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3423 493 Size: 711 2219 2702 Fragments arranged by size: 2702 2219 711 BsaBI GATnn'nnATC Cuts at: 5626 5626 Size: 5632 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3439 3821 4456 275 Size: 53 83 186 936 1906 382 635 1451 Fragments arranged by size: 1906 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2430 4065 4463 4737 4884 5295 2430 Size: 1635 398 274 147 411 2767 Fragments arranged by size: 2767 1635 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2039 2069 2828 2858 1178 Size: 30 165 30 636 30 759 30 3952 Fragments arranged by size: 3952 759 636 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 1186 Size: 1162 4470 BseYI C'CCAG_C Cuts at: 2100 2120 2311 4835 5331 2100 Size: 20 191 2524 496 2401 Fragments arranged by size: 2524 2401 496 191 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2496 1163 Size: 573 760 4299 Fragments arranged by size: 4299 760 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3082 3843 4871 1540 Size: 275 186 1081 761 1028 2301 Fragments arranged by size: 2301 1081 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2487 3693 3778 4849 2487 Size: 1206 85 1071 3270 Fragments arranged by size: 3270 1206 1071 85 BsmI GAATG_Cn' Cuts at: 3143 3236 3143 Size: 93 5539 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2157 2487 2951 3693 3778 4849 Size: 399 352 15 330 464 742 85 1071 Cuts at: 4849 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 464 399 352 330 85 15 BspEI T'CCGG_A Cuts at: 2430 5295 2430 Size: 2865 2767 BspHI T'CATG_A Cuts at: 3521 5251 3521 Size: 1730 3902 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2034 2616 2987 844 Size: 1190 582 371 3489 Fragments arranged by size: 3489 1190 582 371 BsrBI CCG'CTC Cuts at: 1394 1823 3519 1394 Size: 429 1696 3507 Fragments arranged by size: 3507 1696 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4127 4301 65 Size: 1183 2879 174 1396 Fragments arranged by size: 2879 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4276 4463 1700 Size: 110 2466 187 2869 Fragments arranged by size: 2869 2466 187 110 BsrGI T'GTAC_A Cuts at: 96 2046 96 Size: 1950 3682 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5632 BssSI C'ACGA_G Cuts at: 3692 4704 3692 Size: 1012 4620 BstAPI GCAn_nnn'nTGC Cuts at: 794 2026 794 Size: 1232 4400 BstBI TT'CG_AA Cuts at: 4436 4436 Size: 5632 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5632 BstXI CCAn_nnnn'nTGG Cuts at: 2236 2236 Size: 5632 BstYI r'GATC_y Cuts at: 1972 2469 2962 3329 3714 3731 5183 5269 5281 Size: 497 493 367 385 17 1452 86 12 Cuts at: 5281 5627 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 497 493 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2235 513 Size: 552 1170 3910 Fragments arranged by size: 3910 1170 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4893 Fragments arranged by size: 4893 683 56 BtsI GCAGTG_nn' Cuts at: 3148 3942 3962 3148 Size: 794 20 4818 Fragments arranged by size: 4818 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5632 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5597 DraI TTT'AAA Cuts at: 2936 2936 Size: 5632 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4639 809 Size: 507 3323 1802 Fragments arranged by size: 3323 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2243 3079 3968 Size: 54 1425 50 275 18 413 836 889 Cuts at: 3968 5597 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 836 413 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3079 1537 Size: 275 1267 4090 Fragments arranged by size: 4090 1267 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3562 1353 Size: 2209 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2099 4219 4593 4739 387 Size: 1064 138 510 2120 374 146 1280 Fragments arranged by size: 2120 1280 1064 510 374 146 138 EcoICRI GAG'CTC Cuts at: 2485 2485 Size: 5632 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2549 2570 2855 2924 3695 4292 5079 Size: 393 917 21 285 69 771 597 787 Cuts at: 5079 5347 1239 Size: 268 1524 Fragments arranged by size: 1524 917 787 771 597 393 285 268 69 21 EcoNI CCTnn'n_nnAGG Cuts at: 2030 2210 2030 Size: 180 5452 EcoO109I rG'GnC_Cy Cuts at: 2494 2524 2494 Size: 30 5602 EcoRI G'AATT_C Cuts at: 2171 2941 3319 2171 Size: 770 378 4484 Fragments arranged by size: 4484 770 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2056 2056 Size: 5632 FspI TGC'GCA Cuts at: 4138 4138 Size: 5632 HaeII r_GCGC'y Cuts at: 1678 2107 4779 1678 Size: 429 2672 2531 Fragments arranged by size: 2672 2531 429 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2312 2344 2724 2756 2868 Size: 32 135 32 740 32 380 32 112 Cuts at: 2868 2900 4273 4305 4347 4379 1373 Size: 32 1373 32 42 32 2626 Fragments arranged by size: 2626 1373 740 380 135 112 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2722 2976 3222 669 Size: 988 281 784 254 246 3079 Fragments arranged by size: 3079 988 784 281 254 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5632 HpaI GTT'AAC Cuts at: 3222 3222 Size: 5632 KpnI G_GTAC'C Cuts at: 2957 2957 Size: 5632 MfeI C'AATT_G Cuts at: 3231 3231 Size: 5632 MluI A'CGCG_T Cuts at: 5508 5508 Size: 5632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4746 4930 1305 Size: 3441 184 2007 Fragments arranged by size: 3441 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3810 Fragments arranged by size: 3810 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2084 2234 2507 2768 3590 3949 4108 Size: 648 918 150 273 261 822 359 159 Cuts at: 4108 518 Size: 2042 Fragments arranged by size: 2042 918 822 648 359 273 261 159 150 MspA1I CmG'CkG Cuts at: 1606 3048 3725 4873 5118 1606 Size: 1442 677 1148 245 2120 Fragments arranged by size: 2120 1442 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5080 NdeI CA'TA_TG Cuts at: 387 387 Size: 5632 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5632 NotI GC'GGCC_GC Cuts at: 3079 3079 Size: 5632 NsiI A_TGCA'T Cuts at: 2863 2863 Size: 5632 NspI r_CATG'y Cuts at: 1132 1229 2089 2845 2990 4535 1132 Size: 97 860 756 145 1545 2229 Fragments arranged by size: 2229 1545 860 756 145 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5632 PciI A'CATG_T Cuts at: 4531 4531 Size: 5632 PflMI CCAn_nnn'nTGG Cuts at: 1108 2390 1108 Size: 1282 4350 PmeI GTTT'AAAC Cuts at: 2936 2936 Size: 5632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2828 2860 3701 3733 5214 5246 2828 Size: 32 841 32 1481 32 3214 Fragments arranged by size: 3214 1481 841 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2494 2524 2494 Size: 30 5602 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5632 PsiI TTA'TAA Cuts at: 3202 3202 Size: 5632 PstI C_TGCA'G Cuts at: 830 2031 2613 2916 2984 830 Size: 1201 582 303 68 3478 Fragments arranged by size: 3478 1201 582 303 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5632 SacI G_AGCT'C Cuts at: 2487 2487 Size: 5632 SalI G'TCGA_C Cuts at: 1936 2974 1936 Size: 1038 4594 SbfI CC_TGCA'GG Cuts at: 2613 2984 2613 Size: 371 5261 ScaI AGT'ACT Cuts at: 3880 5592 3880 Size: 1712 3920 SfcI C'TryA_G Cuts at: 826 1045 2027 2609 2912 2980 4115 4796 4987 Size: 219 982 582 303 68 1135 681 191 Cuts at: 4987 826 Size: 1471 Fragments arranged by size: 1471 1135 982 681 582 303 219 191 68 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5632 SmaI CCC'GGG Cuts at: 1872 2959 1872 Size: 1087 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2486 3736 4637 4899 820 Size: 197 926 543 1250 901 262 1553 Fragments arranged by size: 1553 1250 926 901 543 262 197 SnaBI TAC'GTA Cuts at: 493 3423 493 Size: 2930 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5632 SphI G_CATG'C Cuts at: 1132 2990 1132 Size: 1858 3774 SspI AAT'ATT Cuts at: 5 52 3556 5 Size: 47 3504 2081 Fragments arranged by size: 3504 2081 47 StuI AGG'CCT Cuts at: 1517 2645 1517 Size: 1128 4504 StyI C'CwwG_G Cuts at: 513 1065 2058 3071 513 Size: 552 993 1013 3074 Fragments arranged by size: 3074 1013 993 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2303 3659 3676 3829 4014 4459 325 Size: 1255 723 1356 17 153 185 445 1498 Fragments arranged by size: 1498 1356 1255 723 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2046 3878 5590 Size: 274 80 33 51 1443 69 1832 1712 Cuts at: 5590 96 Size: 138 Fragments arranged by size: 1832 1712 1443 274 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5592 5624 5592 Size: 32 5600 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5632 XbaI T'CTAG_A Cuts at: 2968 2968 Size: 5632 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5632 XmaI C'CCGG_G Cuts at: 1870 2957 1870 Size: 1087 4545 XmnI GAAnn'nnTTC Cuts at: 2036 2438 2657 3761 2036 Size: 402 219 1104 3907 Fragments arranged by size: 3907 1104 402 219 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3440 276 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI