################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BanI BanII BbeI BbrPI BcgI BclI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FokI FseI HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MunI NarI NdeI NdeII NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp509I Tth111I TthHB8I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 293 CGCG CG^CG AfaI 389 GTAC GT^AC Alw44I 271 GTGCAC G^TGCAC AlwNI 10 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 271 GTGCAC G^TGCAC AviII 238 TGCGCA TGC^GCA BbsI 313 GTCTTC GAAGAC(2/6) BbvCI 169 GCTGAGG CCTCAGC(-5/-2) BciVI 117 GGATAC GTATCC(6/5) BfaI 358 CTAG C^TAG BlpI 7 GCTCAGC GC^T[ACGT]AGC BpmI 318 CTCCAG CTGGAG(16/14) Bpu1102I 7 GCTCAGC GC^T[ACGT]AGC BpuAI 313 GTCTTC GAAGAC(2/6) BseRI 172 GAGGAG GAGGAG(10/8) BspMI 123 GCAGGT ACCTGC(4/8) BsrBI 145 CCGCTC CCGCTC(-3/-3) BsrFI 184 GCCGGC [GA]^CCGG[CT] BstUI 293 CGCG CG^CG CelII 7 GCTCAGC GC^T[ACGT]AGC Cfr10I 184 GCCGGC [GA]^CCGG[CT] EcoT14I 107 CCATGG C^C[AT][AT]GG EcoT22I 542 ATGCAT ATGCA^T FspI 238 TGCGCA TGC^GCA HaeII 348 AGCGCC [GA]GCGC^[CT] HindIII 89 AAGCTT A^AGCTT HphI 129 GGTGA GGTGA(8/7) MaeI 358 CTAG C^TAG MvnI 293 CGCG CG^CG NaeI 184 GCCGGC GCC^GGC NcoI 107 CCATGG C^CATGG NgoMIV 184 GCCGGC G^CCGGC NsiI 542 ATGCAT ATGCA^T PflMI 107 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 208 CTGCAG CTGCA^G PvuII 193 CAGCTG CAG^CTG RsaI 389 GTAC GT^AC ScaI 388 AGTACT AGT^ACT SfcI 208 CTGCAG C^T[GA][CT]AG SmaI 47 CCCGGG CCC^GGG SmlI 471 CTTGAG C^T[CT][GA]AG StyI 107 CCATGG C^C[AT][AT]GG Van91I 107 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 494 CCAGTGACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 47 CCCGGG C^CCGGG XmaI 47 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AspHI 256 GTGCTC G[AT]GC[AT]^C 271 GTGCAC AvaI 47 CCCGGG C^[CT]CG[GA]G 301 CTCGGG BcnI 47 CCCGG CC^[GC]GG 48 CCGGG BglI 12 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 198 GCCGTCTTGGC BmyI 256 GTGCTC G[AGT]GC[ACT]^C 271 GTGCAC BsiHKAI 256 GTGCTC G[AT]GC[AT]^C 271 GTGCAC BsoBI 47 CCCGGG C^[CT]CG[GA]G 301 CTCGGG Bsp1286I 256 GTGCTC G[AGT]GC[ACT]^C 271 GTGCAC BstNI 281 CCTGG CC^[AT]GG 462 CCTGG DraII 136 GGGTCCT [GA]G^G[ACGT]CC[CT] 284 GGGACCT DraIII 29 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG 250 CACGCAGTG DsaI 107 CCATGG C^C[GA][CT]GG 308 CCGTGG EcoO109I 136 GGGTCCT [GA]G^G[ACGT]CC[CT] 284 GGGACCT EcoRII 281 CCTGG ^CC[AT]GG 462 CCTGG FauI 154 CCCGC CCCGC(4/6) 189 CCCGC HapII 48 CCGG C^CGG 185 CCGG HpaII 48 CCGG C^CGG 185 CCGG MaeII 30 ACGT A^CGT 335 ACGT MseI 520 TTAA T^TAA 628 TTAA MspA1I 13 CCGCTG C[AC]G^C[GT]G 193 CAGCTG MspI 48 CCGG C^CGG 185 CCGG MvaI 281 CCTGG CC^[AT]GG 462 CCTGG NciI 47 CCCGG CC^[GC]GG 48 CCGGG NlaIII 108 CATG CATG^ 454 CATG PleI 265 GACTC GAGTC(4/5) 660 GACTC PpuMI 136 GGGTCCT [GA]G^G[AT]CC[CT] 284 GGGACCT SfaNI 21 GCATC GCATC(5/9) 544 GCATC TfiI 423 GATTC G^A[AT]TC 645 GATTC Tru9I 520 TTAA T^TAA 628 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AluI AvaII BbvI BsaJI BsiYI BslI BsmFI BsrI BssKI Cac8I CfoI Cfr13I DdeI Fnu4HI HaeIII HhaI HinP1I HinfI ItaI MaeIII MboII MnlI MwoI NlaIV Sau96I ScrFI TseI Tsp45I TspRI