Restriction map for
pFN21AE2361
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstF5I BstXI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MroI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 298 CGCG CG^CG
AlwI 618 GATCC GGATC(4/5)
AocI 697 CCTCAGG CC^T[ACGT]AGG
Aor51HI 219 AGCGCT AGC^GCT
ApaI 585 GGGCCC GGGCC^C
AspHI 712 GAGCAC G[AT]GC[AT]^C
BalI 557 TGGCCA TGG^CCA
BanII 585 GGGCCC G[GA]GC[CT]^C
BlpI 10 GCTCAGC GC^T[ACGT]AGC
BpmI 16 CTGGAG CTGGAG(16/14)
Bpu1102I 10 GCTCAGC GC^T[ACGT]AGC
BsaI 80 GAGACC GGTCTC(1/5)
BsgI 396 CTGCAC GTGCAG(16/14)
BsiHKAI 712 GAGCAC G[AT]GC[AT]^C
BsmFI 171 GTCCC GGGAC(10/14)
BspHI 387 TCATGA T^CATGA
BspMI 432 GCAGGT ACCTGC(4/8)
BsrDI 606 CATTGC GCAATG(2/0)
BstUI 298 CGCG CG^CG
BstYI 617 AGATCC [GA]^GATC[CT]
Bsu36I 697 CCTCAGG CC^T[ACGT]AGG
CelII 10 GCTCAGC GC^T[ACGT]AGC
EarI 598 GAAGAG CTCTTC(1/4)
Eco47III 219 AGCGCT AGC^GCT
Eco81I 697 CCTCAGG CC^T[ACGT]AGG
EcoNI 112 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 494 CCAAGG C^C[AT][AT]GG
FauI 156 CCCGC CCCGC(4/6)
HinfI 136 GAGTC G^A[ACGT]TC
Ksp632I 598 GAAGAG CTCTTC(1/4)
MflI 617 AGATCC [GA]^GATC[CT]
MluNI 557 TGGCCA TGG^CCA
MscI 557 TGGCCA TGG^CCA
MspA1I 13 CAGCTG C[AC]G^C[GT]G
MvnI 298 CGCG CG^CG
PleI 136 GAGTC GAGTC(4/5)
PstI 140 CTGCAG CTGCA^G
PvuII 13 CAGCTG CAG^CTG
RcaI 387 TCATGA T^CATGA
SmlI 215 CTCAAG C^T[CT][GA]AG
StyI 494 CCAAGG C^C[AT][AT]GG
XcmI 611 CCACGCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 617 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 237 GTAC GT^AC
453 GTAC
AlwNI 616 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
775 CAGGCTCTG
BcnI 89 CCCGG CC^[GC]GG
630 CCGGG
BsmAI 80 GAGAC GTCTC(1/5)
326 GAGAC
BsrBI 185 CCGCTC CCGCTC(-3/-3)
27 GAGCGG
BstEII 501 GGTCACC G^GT[ACGT]ACC
577 GGTTACC
BstPI 501 GGTCACC G^GT[ACGT]ACC
577 GGTTACC
Cac8I 625 GCCTGC GC[ACGT]^[ACGT]GC
774 GCAGGC
DpnII 385 GATC ^GATC
618 GATC
DraII 443 AGGCCCT [GA]G^G[ACGT]CC[CT]
585 GGGCCCT
EcoO109I 443 AGGCCCT [GA]G^G[ACGT]CC[CT]
585 GGGCCCT
EcoO65I 501 GGTCACC G^GT[ACGT]ACC
577 GGTTACC
HphI 369 GGTGA GGTGA(8/7)
503 TCACC
MboI 385 GATC ^GATC
618 GATC
MboII 382 GAAGA GAAGA(8/7)
598 GAAGA
NciI 89 CCCGG CC^[GC]GG
630 CCGGG
NdeII 385 GATC ^GATC
618 GATC
RsaI 237 GTAC GT^AC
453 GTAC
Sau3AI 385 GATC ^GATC
618 GATC
SfaNI 354 GATGC GCATC(5/9)
520 GATGC
SfiI 95 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
549 GGCCTATGTGGCC
Tsp45I 370 GTGAC ^GT[GC]AC
502 GTCAC
TspRI 473 CAGTG CA[GC]TG[ACGT][ACGT]^
536 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BanI BbvI BglI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI CfoI Cfr13I DdeI
EaeI EcoRII Fnu4HI HaeII HaeIII
HapII HgaI HhaI HinP1I HpaII
ItaI MaeIII MnlI MspI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfcI TseI