################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI AspEI AvrII BalI BamHI BbrPI BbvCI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaI BseAI BseRI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BsrI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HindIII HpaI HphI Ksp632I MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfcI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 164 GGTACC G^GTACC AlwNI 514 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 438 CCTGAGG CC^T[ACGT]AGG Asp718I 164 GGTACC G^GTACC AspI 445 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 379 CCCGAG C^[CT]CG[GA]G AvaII 471 GGACC G^G[AT]CC BbsI 313 GTCTTC GAAGAC(2/6) BciVI 303 GGATAC GTATCC(6/5) BglI 482 GCCGGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 634 CTGGAG CTGGAG(16/14) BpuAI 313 GTCTTC GAAGAC(2/6) BsaWI 231 TCCGGT [AT]^CCGG[AT] BsgI 337 GTGCAG GTGCAG(16/14) BsmI 648 GAATGC GAATGC(1/-1) BsoBI 379 CCCGAG C^[CT]CG[GA]G BspMI 154 ACCTGC ACCTGC(4/8) BsrBI 37 CCGCTC CCGCTC(-3/-3) BsrDI 158 GCAATG GCAATG(2/0) BsrFI 179 ACCGGC [GA]^CCGG[CT] BstAPI 92 GCATCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 222 GGATG GGATG(2/0) BstXI 185 CCACTGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 438 CCTGAGG CC^T[ACGT]AGG Cfr10I 179 ACCGGC [GA]^CCGG[CT] EaeI 521 TGGCCG [CT]^GGCC[GA] Eco81I 438 CCTGAGG CC^T[ACGT]AGG FokI 222 GGATG GGATG(9/13) HgaI 567 GACGC GACGC(5/10) HincII 451 GTCAAC GT[CT]^[GA]AC HindII 451 GTCAAC GT[CT]^[GA]AC HinfI 595 GACTC G^A[ACGT]TC KpnI 164 GGTACC GGTAC^C MaeII 455 ACGT A^CGT PflFI 445 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 595 GACTC GAGTC(4/5) PvuII 466 CAGCTG CAG^CTG SfaNI 92 GCATC GCATC(5/9) SfiI 481 GGCCGGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 550 CTTGAG C^T[CT][GA]AG Tsp509I 462 AATT ^AATT Tth111I 445 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 118 GGCGCC G[GA]^CG[CT]C 405 GGCGCC AfaI 165 GTAC GT^AC 457 GTAC AlwI 384 GATCC GGATC(4/5) 531 GATCC ApaI 44 GGGCCC GGGCC^C 489 GGGCCC Asp700I 687 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 690 GAAGTTCTTC AviII 236 TGCGCA TGC^GCA 662 TGCGCA BbeI 118 GGCGCC GGCGC^C 405 GGCGCC BcgI 535 CGAGCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 134 GCAGCCTCATCG BlpI 15 GCTGAGC GC^T[ACGT]AGC 557 GCTCAGC Bpu1102I 15 GCTGAGC GC^T[ACGT]AGC 557 GCTCAGC BsaHI 118 GGCGCC G[GA]^CG[CT]C 405 GGCGCC BssHII 74 GCGCGC G^CGCGC 172 GCGCGC BstYI 383 AGATCC [GA]^GATC[CT] 530 AGATCC CelII 15 GCTGAGC GC^T[ACGT]AGC 557 GCTCAGC DpnII 384 GATC ^GATC 531 GATC FspI 236 TGCGCA TGC^GCA 662 TGCGCA HaeII 118 GGCGCC [GA]GCGC^[CT] 405 GGCGCC Hin1I 118 GGCGCC G[GA]^CG[CT]C 405 GGCGCC KasI 118 GGCGCC G^GCGCC 405 GGCGCC KspI 7 CCGCGG CCGC^GG 61 CCGCGG MboI 384 GATC ^GATC 531 GATC MflI 383 AGATCC [GA]^GATC[CT] 530 AGATCC NarI 118 GGCGCC GG^CGCC 405 GGCGCC NdeII 384 GATC ^GATC 531 GATC RsaI 165 GTAC GT^AC 457 GTAC SacI 227 GAGCTC GAGCT^C 244 GAGCTC SacII 7 CCGCGG CCGC^GG 61 CCGCGG Sau3AI 384 GATC ^GATC 531 GATC SfoI 118 GGCGCC GGC^GCC 405 GGCGCC TspRI 186 CACTG CA[GC]TG[ACGT][ACGT]^ 609 CAGTG XhoII 383 AGATCC [GA]^GATC[CT] 530 AGATCC XmnI 687 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 690 GAAGTTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AccII AciI AluI AspHI BanI BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI