(Circular) (Six-base) MAPSORT of: pFN21AE4029.seq Check: 185 from: 1 to: 5413 pFN21AE4029 5413 bp With 182 enzymes: SgfI * March 28, 2012 13:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2168 2168 Size: 5413 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3223 278 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2756 1937 Size: 819 4594 Acc65I G'GTAC_C Cuts at: 2169 2734 2169 Size: 565 4848 AclI AA'CG_TT Cuts at: 1563 3540 3913 1563 Size: 1977 373 3063 Fragments arranged by size: 3063 1977 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2317 3476 4860 5128 1632 Size: 685 1159 1384 268 1917 Fragments arranged by size: 1917 1384 1159 685 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5216 AflIII A'CryG_T Cuts at: 1203 4312 5289 1203 Size: 3109 977 1327 Fragments arranged by size: 3109 1327 977 AgeI A'CCGG_T Cuts at: 4244 4244 Size: 5413 AhdI GACnn_n'nnGTC Cuts at: 4142 4142 Size: 5413 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5413 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5413 AlwNI CAG_nnn'CTG Cuts at: 2524 3156 4728 2524 Size: 632 1572 3209 Fragments arranged by size: 3209 1572 632 ApaI G_GGCC'C Cuts at: 2053 2498 2053 Size: 445 4968 ApaLI G'TGCA_C Cuts at: 3470 4626 3470 Size: 1156 4257 ApoI r'AATT_y Cuts at: 1493 2722 2948 3100 5209 1493 Size: 1229 226 152 2109 1697 Fragments arranged by size: 2109 1697 1229 226 152 AseI AT'TA_AT Cuts at: 160 1032 3967 160 Size: 872 2935 1606 Fragments arranged by size: 2935 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 AvaI C'yCGr_G Cuts at: 1870 1943 2384 2738 1870 Size: 73 441 354 4545 Fragments arranged by size: 4545 441 354 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2301 2334 2301 Size: 33 5380 BamHI G'GATC_C Cuts at: 2743 3110 2743 Size: 367 5046 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 2169 2410 2429 2734 Size: 325 217 627 336 46 241 19 305 Cuts at: 2734 4189 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 336 325 305 241 217 46 19 BanII G_rGCy'C Cuts at: 1391 2053 2236 2253 2498 2732 1391 Size: 662 183 17 245 234 4072 Fragments arranged by size: 4072 662 245 234 183 17 BbeI G_GCGC'C Cuts at: 2127 2414 2127 Size: 287 5126 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2311 928 Size: 971 412 4030 Fragments arranged by size: 4030 971 412 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2128 2162 2529 2563 3604 Size: 34 50 34 107 34 367 34 1041 Cuts at: 3604 3638 1903 Size: 34 3678 Fragments arranged by size: 3678 1041 367 107 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2302 3305 4515 2302 Size: 1003 1210 3200 Fragments arranged by size: 3200 1210 1003 BclI T'GATC_A Cuts at: 1548 1548 Size: 5413 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2493 4024 136 Size: 107 122 71 631 605 821 1531 1525 Fragments arranged by size: 1531 1525 821 631 605 122 107 71 BglII A'GATC_T Cuts at: 5408 5408 Size: 5413 BlpI GC'TnA_GC Cuts at: 2021 2563 2830 2021 Size: 542 267 4604 Fragments arranged by size: 4604 542 267 Bme1580I G_kGCm'C Cuts at: 1790 2053 2432 2498 2505 3474 4630 1790 Size: 263 379 66 7 969 1156 2573 Fragments arranged by size: 2573 1156 969 379 263 66 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5413 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3144 4102 442 Size: 416 53 83 399 24 1727 958 1753 Fragments arranged by size: 1753 1727 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5413 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2660 4073 1239 Size: 1421 1413 2579 Fragments arranged by size: 2579 1421 1413 Bpu10I CC'TnA_GC Cuts at: 2517 2517 Size: 5413 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2576 3538 4403 4701 2576 Size: 962 865 298 3288 Fragments arranged by size: 3288 962 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4076 882 Size: 617 2577 2219 Fragments arranged by size: 2577 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3204 493 Size: 711 2000 2702 Fragments arranged by size: 2702 2000 711 BsaBI GATnn'nnATC Cuts at: 5407 5407 Size: 5413 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2124 2411 3220 3602 Size: 53 83 186 936 591 287 809 382 Cuts at: 3602 4237 275 Size: 635 1451 Fragments arranged by size: 1451 936 809 635 591 382 287 186 83 53 BsaWI w'CCGG_w Cuts at: 2236 3846 4244 4518 4665 5076 2236 Size: 1610 398 274 147 411 2573 Fragments arranged by size: 2573 1610 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2223 2253 1178 Size: 30 165 30 820 30 4338 Fragments arranged by size: 4338 820 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5413 BseYI C'CCAG_C Cuts at: 2092 2348 4616 5112 2092 Size: 256 2268 496 2393 Fragments arranged by size: 2393 2268 496 256 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2363 1163 Size: 573 627 4213 Fragments arranged by size: 4213 627 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2863 3624 4652 1540 Size: 275 186 862 761 1028 2301 Fragments arranged by size: 2301 1028 862 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2236 2253 2564 2628 3474 3559 4630 2236 Size: 17 311 64 846 85 1071 3019 Fragments arranged by size: 3019 1071 846 311 85 64 17 BsmI GAATG_Cn' Cuts at: 2659 2924 3017 2659 Size: 265 93 5055 Fragments arranged by size: 5055 265 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2236 2253 2432 2498 2505 2564 Size: 399 263 183 17 179 66 7 59 Cuts at: 2564 2628 2732 3474 3559 4630 1391 Size: 64 104 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 263 183 179 104 85 66 64 59 17 7 BspEI T'CCGG_A Cuts at: 5076 5076 Size: 5413 BspHI T'CATG_A Cuts at: 3302 5032 3302 Size: 1730 3683 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2168 2768 844 Size: 1324 600 3489 Fragments arranged by size: 3489 1324 600 BsrBI CCG'CTC Cuts at: 1394 1823 2044 3300 1394 Size: 429 221 1256 3507 Fragments arranged by size: 3507 1256 429 221 BsrDI GCAATG_nn' Cuts at: 65 1248 2170 3908 4082 65 Size: 1183 922 1738 174 1396 Fragments arranged by size: 1738 1396 1183 922 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2184 4057 4244 1700 Size: 110 374 1873 187 2869 Fragments arranged by size: 2869 1873 374 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5413 BssHII G'CGCG_C Cuts at: 1925 2079 2177 1925 Size: 154 98 5161 Fragments arranged by size: 5161 154 98 BssSI C'ACGA_G Cuts at: 3473 4485 3473 Size: 1012 4401 BstAPI GCAn_nnn'nTGC Cuts at: 794 2103 794 Size: 1309 4104 BstBI TT'CG_AA Cuts at: 4217 4217 Size: 5413 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5413 BstXI CCAn_nnnn'nTGG Cuts at: 2197 2197 Size: 5413 BstYI r'GATC_y Cuts at: 1972 2388 2535 2743 3110 3495 3512 4964 5050 Size: 416 147 208 367 385 17 1452 86 Cuts at: 5050 5062 5408 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 416 385 367 346 208 147 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2444 2444 Size: 5413 BtgI C'CryG_G Cuts at: 513 1065 2012 2066 2127 513 Size: 552 947 54 61 3799 Fragments arranged by size: 3799 947 552 61 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4674 Fragments arranged by size: 4674 683 56 BtsI GCAGTG_nn' Cuts at: 2190 2929 3723 3743 2190 Size: 739 794 20 3860 Fragments arranged by size: 3860 794 739 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5413 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5378 DraI TTT'AAA Cuts at: 2717 2717 Size: 5413 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4420 809 Size: 507 3104 1802 Fragments arranged by size: 3104 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2526 2860 3749 Size: 54 1425 50 275 18 696 334 889 Cuts at: 3749 5378 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 696 334 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2860 1537 Size: 275 1048 4090 Fragments arranged by size: 4090 1048 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3343 1353 Size: 1990 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4000 4374 4520 387 Size: 1064 138 2411 374 146 1280 Fragments arranged by size: 2411 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2234 2251 2234 Size: 17 5396 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2317 2660 3476 4073 4860 5128 1239 Size: 393 685 343 816 597 787 268 1524 Fragments arranged by size: 1524 816 787 685 597 393 343 268 EcoO109I rG'GnC_Cy Cuts at: 2050 2494 2495 2050 Size: 444 1 4968 Fragments arranged by size: 4968 444 1 EcoRI G'AATT_C Cuts at: 2722 3100 2722 Size: 378 5035 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2716 2716 Size: 5413 FspI TGC'GCA Cuts at: 2243 2669 3919 2243 Size: 426 1250 3737 Fragments arranged by size: 3737 1250 426 FspAI rTGC'GCAy Cuts at: 2243 2669 2243 Size: 426 4987 HaeII r_GCGC'y Cuts at: 1678 2127 2414 4560 1678 Size: 449 287 2146 2531 Fragments arranged by size: 2531 2146 449 287 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 4054 4086 4128 Size: 32 135 32 620 32 1830 32 42 Cuts at: 4128 4160 1373 Size: 32 2626 Fragments arranged by size: 2626 1830 620 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2458 2757 3003 669 Size: 988 281 520 299 246 3079 Fragments arranged by size: 3079 988 520 299 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5413 HpaI GTT'AAC Cuts at: 3003 3003 Size: 5413 KasI G'GCGC_C Cuts at: 2123 2410 2123 Size: 287 5126 KpnI G_GTAC'C Cuts at: 2173 2738 2173 Size: 565 4848 MfeI C'AATT_G Cuts at: 3012 3012 Size: 5413 MluI A'CGCG_T Cuts at: 5289 5289 Size: 5413 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4527 4711 1305 Size: 3222 184 2007 Fragments arranged by size: 3222 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3591 Fragments arranged by size: 3591 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3371 3730 3889 518 Size: 648 2205 359 159 2042 Fragments arranged by size: 2205 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2068 2264 2473 2682 2688 2829 3506 Size: 408 54 196 209 209 6 141 677 Cuts at: 3506 4654 4899 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 408 245 209 209 196 141 54 6 NarI GG'CG_CC Cuts at: 2124 2411 2124 Size: 287 5126 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4861 NdeI CA'TA_TG Cuts at: 387 387 Size: 5413 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5413 NotI GC'GGCC_GC Cuts at: 2860 2860 Size: 5413 NspI r_CATG'y Cuts at: 1132 1229 2771 4316 1132 Size: 97 1542 1545 2229 Fragments arranged by size: 2229 1545 1542 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5413 PciI A'CATG_T Cuts at: 4312 4312 Size: 5413 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5413 PmeI GTTT'AAAC Cuts at: 2717 2717 Size: 5413 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3482 3514 4995 5027 3482 Size: 32 1481 32 3868 Fragments arranged by size: 3868 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5413 PsiI TTA'TAA Cuts at: 2983 2983 Size: 5413 PspOMI G'GGCC_C Cuts at: 2049 2494 2049 Size: 445 4968 PstI C_TGCA'G Cuts at: 830 2765 830 Size: 1935 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5413 PvuII CAG'CTG Cuts at: 2473 2473 Size: 5413 SacI G_AGCT'C Cuts at: 2236 2253 2236 Size: 17 5396 SacII CC_GC'GG Cuts at: 2015 2069 2015 Size: 54 5359 SalI G'TCGA_C Cuts at: 1936 2755 1936 Size: 819 4594 SbfI CC_TGCA'GG Cuts at: 2765 2765 Size: 5413 ScaI AGT'ACT Cuts at: 3661 5373 3661 Size: 1712 3701 SfcI C'TryA_G Cuts at: 826 1045 2761 3896 4577 4768 826 Size: 219 1716 1135 681 191 1471 Fragments arranged by size: 1716 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2493 2493 Size: 5413 SfoI GGC'GCC Cuts at: 2125 2412 2125 Size: 287 5126 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5413 SmaI CCC'GGG Cuts at: 1872 2740 1872 Size: 868 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2555 3517 4418 4680 820 Size: 197 926 612 962 901 262 1553 Fragments arranged by size: 1553 962 926 901 612 262 197 SnaBI TAC'GTA Cuts at: 493 3204 493 Size: 2711 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5413 SphI G_CATG'C Cuts at: 1132 2771 1132 Size: 1639 3774 SspI AAT'ATT Cuts at: 5 52 3337 5 Size: 47 3285 2081 Fragments arranged by size: 3285 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5413 StyI C'CwwG_G Cuts at: 513 1065 2852 513 Size: 552 1787 3074 Fragments arranged by size: 3074 1787 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2298 2447 3440 3457 3610 3795 4240 Size: 1255 718 149 993 17 153 185 445 Cuts at: 4240 325 Size: 1498 Fragments arranged by size: 1498 1255 993 718 445 185 153 149 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3659 5371 96 Size: 274 80 33 51 1443 1682 1712 138 Fragments arranged by size: 1712 1682 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2223 2255 5373 5405 2223 Size: 32 3118 32 2231 Fragments arranged by size: 3118 2231 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2453 1316 Size: 1137 4276 XbaI T'CTAG_A Cuts at: 2749 2749 Size: 5413 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5413 XmaI C'CCGG_G Cuts at: 1870 2738 1870 Size: 868 4545 XmnI GAAnn'nnTTC Cuts at: 2696 2699 3542 2696 Size: 3 843 4567 Fragments arranged by size: 4567 843 3 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3221 276 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SrfI SwaI XcmI