##################################
# NO CUT ENZYMES (  215 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       Cfr10I      Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
HpaII       ItaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MspI        
MunI        MvaI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tru9I       TseI        
Tsp45I      TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccII         179  CGCG                 CG^CG
AciI          178  CCGC                 CCGC(-3/-1)
AcsI           55  AAATTT               [GA]^AATT[CT]
ApoI           55  AAATTT               [GA]^AATT[CT]
Asp700I       147  GAAACGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         160  GTGCTC               G[AT]GC[AT]^C
BcgI           27  GCAGGGAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI           83  GCTCAGC              GC^T[ACGT]AGC
BmyI          160  GTGCTC               G[AGT]GC[ACT]^C
BpmI            7  CTCCAG               CTGGAG(16/14)
Bpu1102I       83  GCTCAGC              GC^T[ACGT]AGC
BsiHKAI       160  GTGCTC               G[AT]GC[AT]^C
BsmFI          69  GGGAC                GGGAC(10/14)
Bsp1286I      160  GTGCTC               G[AGT]GC[ACT]^C
BspHI         164  TCATGA               T^CATGA
BspMI          79  ACCTGC               ACCTGC(4/8)
BsrI            9  CCAGT                ACTGG(1/-1)
BstF5I         91  CATCC                GGATG(2/0)
BstUI         179  CGCG                 CG^CG
CelII          83  GCTCAGC              GC^T[ACGT]AGC
CfoI          180  GCGC                 GCG^C
DdeI           84  CTCAG                C^T[ACGT]AG
DpnII          34  GATC                 ^GATC
FokI           91  CATCC                GGATG(9/13)
HhaI          180  GCGC                 GCG^C
HinP1I        180  GCGC                 G^CGC
HphI          137  TCACC                GGTGA(8/7)
MboI           34  GATC                 ^GATC
MboII          51  GAAGA                GAAGA(8/7)
MvnI          179  CGCG                 CG^CG
MwoI           88  GCCCATCCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII          34  GATC                 ^GATC
NlaIII        165  CATG                 CATG^
PleI           38  GAGTC                GAGTC(4/5)
RcaI          164  TCATGA               T^CATGA
Sau3AI         34  GATC                 ^GATC
TfiI          134  GAATC                G^A[AT]TC
Tsp509I        56  AATT                 ^AATT
XmnI          147  GAAACGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

HinfI          38  GAGTC                G^A[ACGT]TC
              134  GAATC               
MnlI           18  CCTC                 CCTC(7/6)
              189  CCTC                
TaqI           36  TCGA                 T^CGA
              126  TCGA                
TthHB8I        36  TCGA                 T^CGA
              126  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################