(Circular) (Six-base) MAPSORT of: pFN21AE6534.seq Check: 1062 from: 1 to: 4897 pFN21AE6534 4897 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2707 278 Size: 53 83 186 936 1171 2468 Fragments arranged by size: 2468 1171 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2240 1937 Size: 303 4594 Acc65I G'GTAC_C Cuts at: 2218 2218 Size: 4897 AclI AA'CG_TT Cuts at: 1563 3024 3397 1563 Size: 1461 373 3063 Fragments arranged by size: 3063 1461 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2075 2960 4344 4612 1632 Size: 443 885 1384 268 1917 Fragments arranged by size: 1917 1384 885 443 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4700 AflIII A'CryG_T Cuts at: 1203 3796 4773 1203 Size: 2593 977 1327 Fragments arranged by size: 2593 1327 977 AgeI A'CCGG_T Cuts at: 3728 3728 Size: 4897 AhdI GACnn_n'nnGTC Cuts at: 3626 3626 Size: 4897 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4897 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4897 AlwNI CAG_nnn'CTG Cuts at: 2640 4212 2640 Size: 1572 3325 ApaLI G'TGCA_C Cuts at: 2954 4110 2954 Size: 1156 3741 ApoI r'AATT_y Cuts at: 1493 2060 2206 2432 2584 4693 1493 Size: 567 146 226 152 2109 1697 Fragments arranged by size: 2109 1697 567 226 152 146 AseI AT'TA_AT Cuts at: 160 1032 3451 160 Size: 872 2419 1606 Fragments arranged by size: 2419 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4897 AvaI C'yCGr_G Cuts at: 1870 1943 2222 1870 Size: 73 279 4545 Fragments arranged by size: 4545 279 73 BamHI G'GATC_C Cuts at: 2227 2594 2227 Size: 367 4530 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2218 3673 618 Size: 325 217 627 431 1455 1842 Fragments arranged by size: 1842 1455 627 431 325 217 BanII G_rGCy'C Cuts at: 1391 2216 1391 Size: 825 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3926 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2021 2055 3088 3122 1903 Size: 34 84 34 1033 34 3678 Fragments arranged by size: 3678 1033 84 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2789 3999 2789 Size: 1210 3687 BclI T'GATC_A Cuts at: 1548 1548 Size: 4897 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3508 136 Size: 107 122 71 631 605 1836 1525 Fragments arranged by size: 1836 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4892 4892 Size: 4897 BlpI GC'TnA_GC Cuts at: 2089 2314 2089 Size: 225 4672 Bme1580I G_kGCm'C Cuts at: 1790 2958 4114 1790 Size: 1168 1156 2573 Fragments arranged by size: 2573 1168 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4897 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2628 3586 442 Size: 416 53 83 399 24 1211 958 1753 Fragments arranged by size: 1753 1211 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4897 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1997 3557 1239 Size: 758 1560 2579 Fragments arranged by size: 2579 1560 758 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3022 3887 4185 3022 Size: 865 298 3734 Fragments arranged by size: 3734 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3560 882 Size: 617 2061 2219 Fragments arranged by size: 2219 2061 617 BsaAI yAC'GTr Cuts at: 493 1204 2688 493 Size: 711 1484 2702 Fragments arranged by size: 2702 1484 711 BsaBI GATnn'nnATC Cuts at: 4891 4891 Size: 4897 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2704 3086 3721 275 Size: 53 83 186 936 1171 382 635 1451 Fragments arranged by size: 1451 1171 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3330 3728 4002 4149 4560 3330 Size: 398 274 147 411 3667 Fragments arranged by size: 3667 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2028 2058 1178 Size: 30 165 30 625 30 4017 Fragments arranged by size: 4017 625 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4897 BseYI C'CCAG_C Cuts at: 2175 4100 4596 2175 Size: 1925 496 2476 Fragments arranged by size: 2476 1925 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4324 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2347 3108 4136 1540 Size: 275 186 346 761 1028 2301 Fragments arranged by size: 2301 1028 761 346 275 186 BsiHKAI G_wGCw'C Cuts at: 2169 2958 3043 4114 2169 Size: 789 85 1071 2952 Fragments arranged by size: 2952 1071 789 85 BsmI GAATG_Cn' Cuts at: 2408 2501 2408 Size: 93 4804 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2216 2958 3043 4114 1391 Size: 399 379 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 379 85 47 BspEI T'CCGG_A Cuts at: 4560 4560 Size: 4897 BspHI T'CATG_A Cuts at: 2169 2786 4516 2169 Size: 617 1730 2550 Fragments arranged by size: 2550 1730 617 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2093 2252 844 Size: 1249 159 3489 Fragments arranged by size: 3489 1249 159 BsrBI CCG'CTC Cuts at: 1394 1823 2784 1394 Size: 429 961 3507 Fragments arranged by size: 3507 961 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3392 3566 65 Size: 1183 2144 174 1396 Fragments arranged by size: 2144 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3541 3728 1700 Size: 110 1731 187 2869 Fragments arranged by size: 2869 1731 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4897 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4897 BssSI C'ACGA_G Cuts at: 2957 3969 2957 Size: 1012 3885 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4897 BstBI TT'CG_AA Cuts at: 3701 3701 Size: 4897 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4897 BstYI r'GATC_y Cuts at: 1972 2227 2594 2979 2996 4448 4534 4546 4892 Size: 255 367 385 17 1452 86 12 346 Cuts at: 4892 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 255 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4345 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4158 Fragments arranged by size: 4158 683 56 BtsI GCAGTG_nn' Cuts at: 2413 3207 3227 2413 Size: 794 20 4083 Fragments arranged by size: 4083 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4897 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4862 DraI TTT'AAA Cuts at: 2201 2201 Size: 4897 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3904 809 Size: 507 2588 1802 Fragments arranged by size: 2588 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2344 3233 4862 Size: 54 1425 50 275 18 514 889 1629 Cuts at: 4862 8 Size: 43 Fragments arranged by size: 1629 1425 889 514 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2344 1537 Size: 275 532 4090 Fragments arranged by size: 4090 532 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2827 1353 Size: 1474 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3484 3858 4004 387 Size: 1064 138 1895 374 146 1280 Fragments arranged by size: 1895 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1997 2075 2960 3557 4344 4612 1239 Size: 393 365 78 885 597 787 268 1524 Fragments arranged by size: 1524 885 787 597 393 365 268 78 EcoRI G'AATT_C Cuts at: 2206 2584 2206 Size: 378 4519 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2173 2173 Size: 4897 FspI TGC'GCA Cuts at: 3403 3403 Size: 4897 HaeII r_GCGC'y Cuts at: 1678 4044 1678 Size: 2366 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2028 2060 2111 2124 2143 Size: 32 135 32 456 32 51 13 19 Cuts at: 2143 2156 3538 3570 3612 3644 1373 Size: 13 1382 32 42 32 2626 Fragments arranged by size: 2626 1382 456 135 51 42 32 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 2241 2487 669 Size: 988 281 303 246 3079 Fragments arranged by size: 3079 988 303 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4897 HpaI GTT'AAC Cuts at: 2487 2487 Size: 4897 KpnI G_GTAC'C Cuts at: 2222 2222 Size: 4897 MfeI C'AATT_G Cuts at: 2496 2496 Size: 4897 MluI A'CGCG_T Cuts at: 4773 4773 Size: 4897 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4011 4195 1305 Size: 2706 184 2007 Fragments arranged by size: 2706 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3075 Fragments arranged by size: 3075 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2855 3214 3373 518 Size: 648 1689 359 159 2042 Fragments arranged by size: 2042 1689 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2313 2990 4138 4383 1606 Size: 707 677 1148 245 2120 Fragments arranged by size: 2120 1148 707 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4345 NdeI CA'TA_TG Cuts at: 387 387 Size: 4897 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4897 NotI GC'GGCC_GC Cuts at: 2344 2344 Size: 4897 NspI r_CATG'y Cuts at: 1132 1229 2255 3800 1132 Size: 97 1026 1545 2229 Fragments arranged by size: 2229 1545 1026 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4897 PciI A'CATG_T Cuts at: 3796 3796 Size: 4897 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4897 PmeI GTTT'AAAC Cuts at: 2201 2201 Size: 4897 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2966 2998 4479 4511 2966 Size: 32 1481 32 3352 Fragments arranged by size: 3352 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4897 PsiI TTA'TAA Cuts at: 2467 2467 Size: 4897 PstI C_TGCA'G Cuts at: 830 2249 830 Size: 1419 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4897 SalI G'TCGA_C Cuts at: 1936 2239 1936 Size: 303 4594 SbfI CC_TGCA'GG Cuts at: 2249 2249 Size: 4897 ScaI AGT'ACT Cuts at: 3145 4857 3145 Size: 1712 3185 SfcI C'TryA_G Cuts at: 826 1045 2245 3380 4061 4252 826 Size: 219 1200 1135 681 191 1471 Fragments arranged by size: 1471 1200 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4897 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4897 SmaI CCC'GGG Cuts at: 1872 2224 1872 Size: 352 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3001 3902 4164 820 Size: 197 926 1058 901 262 1553 Fragments arranged by size: 1553 1058 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2688 493 Size: 2195 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4897 SphI G_CATG'C Cuts at: 1132 2255 1132 Size: 1123 3774 SspI AAT'ATT Cuts at: 5 52 2821 5 Size: 47 2769 2081 Fragments arranged by size: 2769 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4897 StyI C'CwwG_G Cuts at: 513 1065 2336 513 Size: 552 1271 3074 Fragments arranged by size: 3074 1271 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2924 2941 3094 3279 3724 325 Size: 1255 1344 17 153 185 445 1498 Fragments arranged by size: 1498 1344 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3143 4855 96 Size: 274 80 33 51 1443 1166 1712 138 Fragments arranged by size: 1712 1443 1166 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4857 4889 4857 Size: 32 4865 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4897 XbaI T'CTAG_A Cuts at: 2233 2233 Size: 4897 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4897 XmaI C'CCGG_G Cuts at: 1870 2222 1870 Size: 352 4545 XmnI GAAnn'nnTTC Cuts at: 2156 3026 2156 Size: 870 4027 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2705 276 Size: 53 83 186 936 1171 2468 Fragments arranged by size: 2468 1171 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI