(Circular) (Six-base) MAPSORT of: pFN21AE9512.seq Check: 1133 from: 1 to: 6301 pFN21AE9512 6301 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4111 278 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2976 3644 1937 Size: 1039 668 4594 Fragments arranged by size: 4594 1039 668 Acc65I G'GTAC_C Cuts at: 3622 3622 Size: 6301 AclI AA'CG_TT Cuts at: 1563 4428 4801 1563 Size: 2865 373 3063 Fragments arranged by size: 3063 2865 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3042 3493 4364 5748 6016 1632 Size: 1410 451 871 1384 268 1917 Fragments arranged by size: 1917 1410 1384 871 451 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6104 AflIII A'CryG_T Cuts at: 1203 5200 6177 1203 Size: 3997 977 1327 Fragments arranged by size: 3997 1327 977 AgeI A'CCGG_T Cuts at: 5132 5132 Size: 6301 AhdI GACnn_n'nnGTC Cuts at: 5030 5030 Size: 6301 AleI CACnn'nnGTG Cuts at: 1166 2138 1166 Size: 972 5329 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2521 1184 Size: 1337 4964 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2795 2827 2795 Size: 32 6269 AlwNI CAG_nnn'CTG Cuts at: 2760 3104 3334 4044 5616 2760 Size: 344 230 710 1572 3445 Fragments arranged by size: 3445 1572 710 344 230 ApaI G_GGCC'C Cuts at: 2494 2494 Size: 6301 ApaLI G'TGCA_C Cuts at: 4358 5514 4358 Size: 1156 5145 ApoI r'AATT_y Cuts at: 1493 3011 3610 3836 3988 6097 1493 Size: 1518 599 226 152 2109 1697 Fragments arranged by size: 2109 1697 1518 599 226 152 AseI AT'TA_AT Cuts at: 160 1032 4855 160 Size: 872 3823 1606 Fragments arranged by size: 3823 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 2297 3626 1870 Size: 73 354 1329 4545 Fragments arranged by size: 4545 1329 354 73 BamHI G'GATC_C Cuts at: 3631 3998 3631 Size: 367 5934 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 3622 5077 618 Size: 325 217 627 258 1577 1455 1842 Fragments arranged by size: 1842 1577 1455 627 325 258 217 BanII G_rGCy'C Cuts at: 1391 2296 2494 2577 2749 3620 1391 Size: 905 198 83 172 871 4072 Fragments arranged by size: 4072 905 871 198 172 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3521 928 Size: 971 1622 3708 Fragments arranged by size: 3708 1622 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4492 4526 1903 Size: 34 2555 34 3678 Fragments arranged by size: 3678 2555 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4193 5403 4193 Size: 1210 5091 BclI T'GATC_A Cuts at: 1548 2130 2373 1548 Size: 582 243 5476 Fragments arranged by size: 5476 582 243 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2885 4912 136 Size: 107 122 71 631 605 1213 2027 1525 Fragments arranged by size: 2027 1525 1213 631 605 122 107 71 BglII A'GATC_T Cuts at: 2230 6296 2230 Size: 4066 2235 BlpI GC'TnA_GC Cuts at: 2213 2218 2895 3718 2213 Size: 5 677 823 4796 Fragments arranged by size: 4796 823 677 5 Bme1580I G_kGCm'C Cuts at: 1790 2494 4362 5518 1790 Size: 704 1868 1156 2573 Fragments arranged by size: 2573 1868 1156 704 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6301 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2045 2856 3221 Size: 416 53 83 399 24 628 811 365 Cuts at: 3221 3416 4032 4990 442 Size: 195 616 958 1753 Fragments arranged by size: 1753 958 811 628 616 416 399 365 195 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2678 3081 4961 1239 Size: 1439 403 1880 2579 Fragments arranged by size: 2579 1880 1439 403 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2171 4426 5291 5589 2171 Size: 2255 865 298 2883 Fragments arranged by size: 2883 2255 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3173 4964 882 Size: 617 1674 1791 2219 Fragments arranged by size: 2219 1791 1674 617 BsaAI yAC'GTr Cuts at: 493 1204 2338 4092 493 Size: 711 1134 1754 2702 Fragments arranged by size: 2702 1754 1134 711 BsaBI GATnn'nnATC Cuts at: 6295 6295 Size: 6301 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4108 4490 5125 275 Size: 53 83 186 936 2575 382 635 1451 Fragments arranged by size: 2575 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4734 5132 5406 5553 5964 4734 Size: 398 274 147 411 5071 Fragments arranged by size: 5071 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2795 2825 1178 Size: 30 165 30 1392 30 4654 Fragments arranged by size: 4654 1392 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6301 BseYI C'CCAG_C Cuts at: 2289 2494 2790 3269 5504 6000 2289 Size: 205 296 479 2235 496 2590 Fragments arranged by size: 2590 2235 496 479 296 205 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2247 1163 Size: 573 511 5217 Fragments arranged by size: 5217 573 511 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3751 4512 5540 1540 Size: 275 186 1750 761 1028 2301 Fragments arranged by size: 2301 1750 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2837 4362 4447 5518 2837 Size: 1525 85 1071 3620 Fragments arranged by size: 3620 1525 1071 85 BsmI GAATG_Cn' Cuts at: 3812 3905 3812 Size: 93 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2296 2494 2577 2749 2837 3620 4362 Size: 399 506 198 83 172 88 783 742 Cuts at: 4362 4447 5518 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 783 742 506 399 198 172 88 85 83 BspEI T'CCGG_A Cuts at: 5964 5964 Size: 6301 BspHI T'CATG_A Cuts at: 2157 2358 2451 2628 4190 5920 2157 Size: 201 93 177 1562 1730 2538 Fragments arranged by size: 2538 1730 1562 201 177 93 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2217 3656 844 Size: 1373 1439 3489 Fragments arranged by size: 3489 1439 1373 BsrBI CCG'CTC Cuts at: 1394 1823 4188 1394 Size: 429 2365 3507 Fragments arranged by size: 3507 2365 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2100 2152 2598 4796 4970 65 Size: 1183 852 52 446 2198 174 1396 Fragments arranged by size: 2198 1396 1183 852 446 174 52 BsrFI r'CCGG_y Cuts at: 1700 1810 4945 5132 1700 Size: 110 3135 187 2869 Fragments arranged by size: 3135 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6301 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6301 BssSI C'ACGA_G Cuts at: 4361 5373 4361 Size: 1012 5289 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6301 BstBI TT'CG_AA Cuts at: 5105 5105 Size: 6301 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6301 BstXI CCAn_nnnn'nTGG Cuts at: 2683 2867 3349 2683 Size: 184 482 5635 Fragments arranged by size: 5635 482 184 BstYI r'GATC_y Cuts at: 1972 2230 2556 2756 3631 3998 4383 4400 5852 Size: 258 326 200 875 367 385 17 1452 Cuts at: 5852 5938 5950 6296 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 875 385 367 346 326 258 200 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4810 Fragments arranged by size: 4810 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5562 Fragments arranged by size: 5562 683 56 BtsI GCAGTG_nn' Cuts at: 2592 3817 4611 4631 2592 Size: 1225 794 20 4262 Fragments arranged by size: 4262 1225 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6266 DraI TTT'AAA Cuts at: 3605 3605 Size: 6301 DraIII CAC_nnn'GTG Cuts at: 3202 3338 3432 3202 Size: 136 94 6071 Fragments arranged by size: 6071 136 94 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3120 3416 5308 809 Size: 507 1804 296 1892 1802 Fragments arranged by size: 1892 1804 1802 507 296 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2340 3231 3748 Size: 54 1425 50 275 18 510 891 517 Cuts at: 3748 4637 6266 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 891 889 517 510 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3748 1537 Size: 275 1936 4090 Fragments arranged by size: 4090 1936 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2720 3018 3558 4231 1353 Size: 1367 298 540 673 3423 Fragments arranged by size: 3423 1367 673 540 298 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4888 5262 5408 387 Size: 1064 138 3299 374 146 1280 Fragments arranged by size: 3299 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2678 3042 3081 3493 4364 4961 5748 Size: 393 1046 364 39 412 871 597 787 Cuts at: 5748 6016 1239 Size: 268 1524 Fragments arranged by size: 1524 1046 871 787 597 412 393 364 268 39 EcoNI CCTnn'n_nnAGG Cuts at: 2827 2827 Size: 6301 EcoO109I rG'GnC_Cy Cuts at: 2491 2680 2491 Size: 189 6112 EcoRI G'AATT_C Cuts at: 3011 3610 3988 3011 Size: 599 378 5324 Fragments arranged by size: 5324 599 378 FspI TGC'GCA Cuts at: 4807 4807 Size: 6301 HaeII r_GCGC'y Cuts at: 1678 2880 5448 1678 Size: 1202 2568 2531 Fragments arranged by size: 2568 2531 1202 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4942 4974 5016 5048 1373 Size: 32 135 32 3370 32 42 32 2626 Fragments arranged by size: 3370 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2176 2197 3475 3645 3891 669 Size: 988 281 238 21 1278 170 246 3079 Fragments arranged by size: 3079 1278 988 281 246 238 170 21 HindIII A'AGCT_T Cuts at: 748 748 Size: 6301 HpaI GTT'AAC Cuts at: 2197 3891 2197 Size: 1694 4607 KpnI G_GTAC'C Cuts at: 3626 3626 Size: 6301 MfeI C'AATT_G Cuts at: 2881 3900 2881 Size: 1019 5282 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5415 5599 1305 Size: 4110 184 2007 Fragments arranged by size: 4110 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2342 3233 10 Size: 54 1425 343 510 891 3078 Fragments arranged by size: 3078 1425 891 510 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2138 2279 4259 4618 4777 518 Size: 648 972 141 1980 359 159 2042 Fragments arranged by size: 2042 1980 972 648 359 159 141 MspA1I CmG'CkG Cuts at: 1606 2108 2794 3104 3717 4394 5542 5787 1606 Size: 502 686 310 613 677 1148 245 2120 Fragments arranged by size: 2120 1148 686 677 613 502 310 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4810 Fragments arranged by size: 4810 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6301 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6301 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NspI r_CATG'y Cuts at: 1132 1229 3659 5204 1132 Size: 97 2430 1545 2229 Fragments arranged by size: 2430 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3002 1348 Size: 1654 4647 PciI A'CATG_T Cuts at: 5200 5200 Size: 6301 PflMI CCAn_nnn'nTGG Cuts at: 1108 2068 1108 Size: 960 5341 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2795 2827 4370 4402 5883 5915 2795 Size: 32 1543 32 1481 32 3181 Fragments arranged by size: 3181 1543 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2680 2680 Size: 6301 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6301 PsiI TTA'TAA Cuts at: 3871 3871 Size: 6301 PspOMI G'GGCC_C Cuts at: 2490 2490 Size: 6301 PstI C_TGCA'G Cuts at: 830 3653 830 Size: 2823 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6301 PvuII CAG'CTG Cuts at: 2108 3104 2108 Size: 996 5305 SalI G'TCGA_C Cuts at: 1936 3643 1936 Size: 1707 4594 SbfI CC_TGCA'GG Cuts at: 3653 3653 Size: 6301 ScaI AGT'ACT Cuts at: 2516 4549 6261 2516 Size: 2033 1712 2556 Fragments arranged by size: 2556 2033 1712 SfcI C'TryA_G Cuts at: 826 1045 2911 3649 4784 5465 5656 826 Size: 219 1866 738 1135 681 191 1471 Fragments arranged by size: 1866 1471 1135 738 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 3628 1872 Size: 1756 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2186 4405 5306 5568 820 Size: 197 926 243 2219 901 262 1553 Fragments arranged by size: 2219 1553 926 901 262 243 197 SnaBI TAC'GTA Cuts at: 493 4092 493 Size: 3599 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 3659 1132 Size: 2527 3774 SspI AAT'ATT Cuts at: 5 52 4225 5 Size: 47 4173 2081 Fragments arranged by size: 4173 2081 47 StuI AGG'CCT Cuts at: 1517 2810 3255 1517 Size: 1293 445 4563 Fragments arranged by size: 4563 1293 445 StyI C'CwwG_G Cuts at: 513 1065 2004 2676 3234 3740 513 Size: 552 939 672 558 506 3074 Fragments arranged by size: 3074 939 672 558 552 506 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4328 4345 4498 4683 5128 325 Size: 1255 2748 17 153 185 445 1498 Fragments arranged by size: 2748 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2514 3573 4547 Size: 274 80 33 51 1443 537 1059 974 Cuts at: 4547 6259 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1059 974 537 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6261 6293 6261 Size: 32 6269 Tth111I GACn'n_nGTC Cuts at: 1316 2960 1316 Size: 1644 4657 XbaI T'CTAG_A Cuts at: 3637 3637 Size: 6301 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2880 3429 2880 Size: 549 5752 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6301 XmaI C'CCGG_G Cuts at: 1870 3626 1870 Size: 1756 4545 XmnI GAAnn'nnTTC Cuts at: 4430 4430 Size: 6301 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4109 276 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI