(Linear) (Six-base) MAPSORT of: KIEE9512.seq Check: 1014 from: 1 to: 1596 KIEE9512.orf With 182 enzymes: SgfI * December 27, 2011 16:41 .. AccI GT'mk_AC Cuts at: 0 971 1596 Size: 971 625 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1037 1488 1596 Size: 1037 451 108 Fragments arranged by size: 1037 451 108 AleI CACnn'nnGTG Cuts at: 0 133 1596 Size: 133 1463 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 516 1596 Size: 516 1080 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 790 822 1596 Size: 790 32 774 Fragments arranged by size: 790 774 32 AlwNI CAG_nnn'CTG Cuts at: 0 755 1099 1329 1596 Size: 755 344 230 267 Fragments arranged by size: 755 344 267 230 ApaI G_GGCC'C Cuts at: 0 489 1596 Size: 489 1107 ApoI r'AATT_y Cuts at: 0 1006 1596 Size: 1006 590 AvaI C'yCGr_G Cuts at: 0 292 1596 Size: 292 1304 BanI G'GyrC_C Cuts at: 0 40 1596 Size: 40 1556 BanII G_rGCy'C Cuts at: 0 291 489 572 744 1596 Size: 291 198 83 172 852 Fragments arranged by size: 852 291 198 172 83 BbsI GAAGACnn'nnnn_ Cuts at: 0 1516 1596 Size: 1516 80 BclI T'GATC_A Cuts at: 0 125 368 1596 Size: 125 243 1228 Fragments arranged by size: 1228 243 125 BglI GCCn_nnn'nGGC Cuts at: 0 880 1596 Size: 880 716 BglII A'GATC_T Cuts at: 0 225 1596 Size: 225 1371 BlpI GC'TnA_GC Cuts at: 0 208 213 890 1596 Size: 208 5 677 706 Fragments arranged by size: 706 677 208 5 Bme1580I G_kGCm'C Cuts at: 0 489 1596 Size: 489 1107 BmrI ACTGGGnnnn_n' Cuts at: 0 40 851 1216 1411 1596 Size: 40 811 365 195 185 Fragments arranged by size: 811 365 195 185 40 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 673 1076 1596 Size: 673 403 520 Fragments arranged by size: 673 520 403 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 1596 Size: 166 1430 BsaI GGTCTCn'nnnn_ Cuts at: 0 1168 1596 Size: 1168 428 BsaAI yAC'GTr Cuts at: 0 333 1596 Size: 333 1263 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 790 820 1596 Size: 790 30 776 Fragments arranged by size: 790 776 30 BseYI C'CCAG_C Cuts at: 0 284 489 785 1264 1596 Size: 284 205 296 479 332 Fragments arranged by size: 479 332 296 284 205 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 242 1596 Size: 242 1354 BsiHKAI G_wGCw'C Cuts at: 0 832 1596 Size: 832 764 Bsp1286I G_dGCh'C Cuts at: 0 291 489 572 744 832 1596 Size: 291 198 83 172 88 764 Fragments arranged by size: 764 291 198 172 88 83 BspHI T'CATG_A Cuts at: 0 152 353 446 623 1596 Size: 152 201 93 177 973 Fragments arranged by size: 973 201 177 152 93 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 212 1596 Size: 212 1384 BsrDI GCAATG_nn' Cuts at: 0 95 147 593 1596 Size: 95 52 446 1003 Fragments arranged by size: 1003 446 95 52 BstXI CCAn_nnnn'nTGG Cuts at: 0 678 862 1344 1596 Size: 678 184 482 252 Fragments arranged by size: 678 482 252 184 BstYI r'GATC_y Cuts at: 0 225 551 751 1596 Size: 225 326 200 845 Fragments arranged by size: 845 326 225 200 BtsI GCAGTG_nn' Cuts at: 0 587 1596 Size: 587 1009 DraIII CAC_nnn'GTG Cuts at: 0 1197 1333 1427 1596 Size: 1197 136 94 169 Fragments arranged by size: 1197 169 136 94 DrdI GACnn_nn'nnGTC Cuts at: 0 1115 1411 1596 Size: 1115 296 185 Fragments arranged by size: 1115 296 185 EaeI y'GGCC_r Cuts at: 0 335 1226 1596 Size: 335 891 370 Fragments arranged by size: 891 370 335 EarI CTCTTCn'nnn_ Cuts at: 0 715 1013 1553 1596 Size: 715 298 540 43 Fragments arranged by size: 715 540 298 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 673 1037 1076 1488 1596 Size: 673 364 39 412 108 Fragments arranged by size: 673 412 364 108 39 EcoNI CCTnn'n_nnAGG Cuts at: 0 822 1596 Size: 822 774 EcoO109I rG'GnC_Cy Cuts at: 0 486 675 1596 Size: 486 189 921 Fragments arranged by size: 921 486 189 EcoRI G'AATT_C Cuts at: 0 1006 1596 Size: 1006 590 HaeII r_GCGC'y Cuts at: 0 875 1596 Size: 875 721 HincII GTy'rAC Cuts at: 0 171 192 1470 1596 Size: 171 21 1278 126 Fragments arranged by size: 1278 171 126 21 HpaI GTT'AAC Cuts at: 0 192 1596 Size: 192 1404 MfeI C'AATT_G Cuts at: 0 876 1596 Size: 876 720 MscI TGG'CCA Cuts at: 0 337 1228 1596 Size: 337 891 368 Fragments arranged by size: 891 368 337 MslI CAynn'nnrTG Cuts at: 0 133 274 1596 Size: 133 141 1322 Fragments arranged by size: 1322 141 133 MspA1I CmG'CkG Cuts at: 0 103 789 1099 1596 Size: 103 686 310 497 Fragments arranged by size: 686 497 310 103 PasI CC'CwG_GG Cuts at: 0 997 1596 Size: 997 599 PflMI CCAn_nnn'nTGG Cuts at: 0 63 1596 Size: 63 1533 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 790 822 1596 Size: 790 32 774 Fragments arranged by size: 790 774 32 PpuMI rG'GwC_Cy Cuts at: 0 675 1596 Size: 675 921 PspOMI G'GGCC_C Cuts at: 0 485 1596 Size: 485 1111 PvuII CAG'CTG Cuts at: 0 103 1099 1596 Size: 103 996 497 Fragments arranged by size: 996 497 103 ScaI AGT'ACT Cuts at: 0 511 1596 Size: 511 1085 SfcI C'TryA_G Cuts at: 0 906 1596 Size: 906 690 SmlI C'TyrA_G Cuts at: 0 181 1596 Size: 181 1415 StuI AGG'CCT Cuts at: 0 805 1250 1596 Size: 805 445 346 Fragments arranged by size: 805 445 346 StyI C'CwwG_G Cuts at: 0 671 1229 1596 Size: 671 558 367 Fragments arranged by size: 671 558 367 TatI w'GTAC_w Cuts at: 0 509 1568 1596 Size: 509 1059 28 Fragments arranged by size: 1059 509 28 Tth111I GACn'n_nGTC Cuts at: 0 955 1596 Size: 955 641 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 875 1424 1596 Size: 875 549 172 Fragments arranged by size: 875 549 172 Enzymes that do cut: AccI AcuI AleI AlfI AloI AlwNI ApaI ApoI AvaI BanI BanII BbsI BclI BglI BglII BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BstXI BstYI BtsI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII HincII HpaI MfeI MscI MslI MspA1I PasI PflMI PpiI PpuMI PspOMI PvuII ScaI SfcI SmlI StuI StyI TatI Tth111I XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BmgBI BmtI BplI Bpu10I BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI Hin4I HindIII KasI KpnI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI XbaI XhoI XmaI XmnI ZraI